Extracellular adenosine triphosphate is associated with airflow limitation severity and symptoms burden in patients with chronic obstructive pulmonary disease

Hlapčić, Iva; Hulina, Andrea; Bačura, Anita Somborac; Rajković, Marija Grdić; Dugac, Andrea Vukić; Popović‐Grle, Sanja; Rumora, Lada

doi:10.1038/s41598-019-51855-w

Cited by 16 publications

(23 citation statements)

References 33 publications

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“…For calculation of multicomponent indices related to COPD assessment, the following data were collected for COPD patients: body mass index (BMI), score obtained from modified Medical Research Council (mMRC) Dyspnea Scale, number of previous exacerbations and Charlson’s comorbidity index. Afterwards, BODCAT (BMI, airflow obstruction, dyspnea, CAT score), BODEx (BMI, airflow obstruction, dyspnea, previous exacerbations), CODEx (Charlson’s comorbidity index, airflow obstruction, dyspnea, previous exacerbations) and DOSE (dyspnea, airflow obstruction, smoking status, previous exacerbations) were calculated [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…Airflow limitation was diagnosed by spirometry on a Master-Screen Pneumospirometer (Jaeger, Wurzburg, Germany), and airflow obstruction was confirmed if FEV 1 /FVC was lower than 0.70 after three acceptable measurements. Furthermore, diffusing capacity for carbon monoxide (DLCO) was measured three times on a Master-Screen PFT Pro (Jaeger, Wurzburg, Germany), as described before [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

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Association of Plasma Heat Shock Protein 70 with Disease Severity, Smoking and Lung Function of Patients with Chronic Obstructive Pulmonary Disease

Hlapčić

Hulina

Rajković

et al. 2020

JCM

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Extracellular heat shock protein 70 (eHsp70) might modulate immune responses in chronic obstructive pulmonary disease (COPD). The aim of the study was to explore eHsp70 concentration in stable COPD, its association with disease severity and smoking status as well as its diagnostic performance in COPD assessment. Plasma samples were collected from 137 COPD patients and 95 healthy individuals, and concentration of eHsp70 was assessed by commercially available enzyme-linked immunosorbent assay (ELISA) kit (Enzo Life Science, Farmingdale, NY, USA). COPD patients were subdivided regarding airflow obstruction severity and symptoms severity according to the Global Initiative for COPD (GOLD) guidelines. eHsp70 concentration increased in COPD patients when compared to controls and increased with the severity of airflow limitation as well as symptoms burden and exacerbation history. eHsp70 concentration did not differ among COPD patients based on smoking status, yet it increased in healthy smokers compared to healthy nonsmokers. In addition, eHsp70 negatively correlated with lung function parameters forced expiratory volume in one second (FEV1) and FEV1/ forced vital capacity (FVC), and positively with COPD multicomponent indices BODCAT (BMI, airflow obstruction, dyspnea, CAT score), BODEx (BMI, airflow obstruction, dyspnea, previous exacerbations), CODEx (Charlson’s comorbidity index, airflow obstruction, dyspnea, previous exacerbations) and DOSE (dyspnea, airflow obstruction, smoking status, previous exacerbations) With great predictive value (OR = 7.63) obtained from univariate logistic regression, eHsp70 correctly classified 76% of cases. eHsp70 is associated with COPD prediction and disease severity and might have the potential for becoming an additional biomarker in COPD assessment.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Association of Plasma Heat Shock Protein 70 with Disease Severity, Smoking and Lung Function of Patients with Chronic Obstructive Pulmonary Disease

Hlapčić

Hulina

Rajković

et al. 2020

JCM

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“…Among them, in 16% of COPD patients inflammation was persistent, and associated with mortality and exacerbations [21]. From our previous studies, we observed that our COPD cohort might also show characteristics of systemic inflammation because of increased concentrations of CRP and Fbg [22] that are being common inflammatory parameters as well as extracellular adenosine triphosphate (eATP) [23] and extracellular heat shock protein 70 (eHsp70) [24] which act like damage-associated molecular patterns (DAMPs). In addition, a previous investigation showed that there were significant associations between the aforementioned parameters with lung function and disease severity, as well as symptoms severity and history of exacerbations, and different multicomponent clinical parameters used for the assessment of dyspnoea, exacerbations and lung impairment.…”

Section: Introductionmentioning

confidence: 96%

Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters

et al. 2020

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Interleukin (IL)-1α, IL-1β, IL-6, IL-8 and tumor necrosis factor (TNF)α contribute to inflammation in chronic obstructive pulmonary disease (COPD). We wanted to investigate their interrelations and association with disease severity, as well as to combine them with other inflammation-associated biomarkers and evaluate their predictive value and potential in identifying various patterns of systemic inflammation. One hundred and nine patients with stable COPD and 95 age- and sex-matched controls were enrolled in the study. Cytokines’ concentrations were determined in plasma samples by antibody-based multiplex immunosorbent assay kits. Investigated cytokines were increased in COPD patients but were not associated with disease or symptoms severity. IL-1β, IL-6 and TNFα showed the best discriminative values regarding ongoing inflammation in COPD. Inflammatory patterns were observed in COPD patients when cytokines, C-reactive protein (CRP), fibrinogen (Fbg), extracellular adenosine triphosphate (eATP), extracellular heat shock protein 70 (eHsp70) and clinical data were included in cluster analysis. IL-1β, eATP and eHsp70 combined correctly classified 91% of cases. Therefore, due to the heterogeneity of COPD, its assessment could be improved by combination of biomarkers. Models including IL-1β, eATP and eHsp70 might identify COPD patients, while IL-1β, IL-6 and TNFα combined with CRP, Fbg, eATP and eHsp70 might be informative regarding various COPD clinical subgroups.

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“…For calculation of multicomponent indices related to COPD assessment, following data were collected for COPD patients: body mass index (BMI), score obtained from modi ed Medical Research Council (mMRC) Dyspnoea Scale, number of previous exacerbations and Charlson's comorbidity index. Afterwards, BODCAT (BMI, air ow obstruction, dyspnoea, CAT score), BODEx (BMI, air ow obstruction, dyspnoea, previous exacerbations), CODEx (Charlson's comorbidity index, air ow obstruction, dyspnoea, previous exacerbations) and DOSE (dyspnoea, air ow obstruction, smoking status, previous exacerbations) were calculated (29).…”

Section: Participantsmentioning

confidence: 99%

“…Air ow limitation was diagnosed by spirometry on a Master-Screen Pneumo spirometer (Jaeger, Germany), and air ow obstruction was con rmed if FEV 1 /FVC was lower than 0.70 after three acceptable measurements. Furthermore, diffusing capacity for carbon monoxide (DLCO) was measured three times on Master-Screen PFT Pro (Jaeger, Wurzburg, Germany), as described before (29).…”

Section: Assessment Of Lung Functionmentioning

confidence: 99%

Association of Plasma Heat Shock Protein 70 with Disease Severity, Smoking and Lung Function of Patients with Chronic Obstructive Pulmonary Disease

Hlapčić

Hulina

Rajković

et al. 2020

Preprint

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Background: Extracellular heat shock protein 70 (eHsp70) acts like a damage-associated molecular pattern (DAMP) and it might modulate immune responses in patients with chronic obstructive pulmonary disease (COPD). The aim of the study was to explore plasma eHsp70 concentration in patients with stable COPD, its association with disease severity and smoking status as well as its diagnostic performance in COPD assessment.Methods: Blood samples were collected from 137 COPD patients and 95 healthy individuals. COPD patients were subdivided into GOLD 2-4 stages based on airflow obstruction severity and GOLD A-D groups regarding symptoms and exacerbations. Concentration of eHsp70 was assessed in EDTA plasma by the commercially available ELISA kit. Statistic analysis was performed by MedCalc statistical software.Results: eHsp70 concentration was increased in COPD patients when compared to controls and was increasing with the severity of airflow limitation as well as symptoms burden and exacerbation history. There were no differences in eHsp70 concentrations among COPD patients based on smoking status, yet eHsp70 was increased in healthy smokers compared to healthy non-smokers. Interestingly, healthy smokers had similar eHsp70 level as COPD patients in GOLD 2 stage and those in GOLD A group. In addition, eHsp70 showed significant negative correlation with lung function parameters FEV1 and FEV1/FVC and positive correlation with COPD multicomponent indices BODCAT, BODEx, CODEx and DOSE. Finally, eHsp70 showed great predictive value (OR=7.63) and correctly classified 76% of cases.Conclusions: Plasma eHsp70 is associated with COPD prediction and disease severity and might have a potential of becoming an additional biomarker in COPD assessment.

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Extracellular adenosine triphosphate is associated with airflow limitation severity and symptoms burden in patients with chronic obstructive pulmonary disease

Cited by 16 publications

References 33 publications

Association of Plasma Heat Shock Protein 70 with Disease Severity, Smoking and Lung Function of Patients with Chronic Obstructive Pulmonary Disease

Association of Plasma Heat Shock Protein 70 with Disease Severity, Smoking and Lung Function of Patients with Chronic Obstructive Pulmonary Disease

Combination of Systemic Inflammatory Biomarkers in Assessment of Chronic Obstructive Pulmonary Disease: Diagnostic Performance and Identification of Networks and Clusters

Association of Plasma Heat Shock Protein 70 with Disease Severity, Smoking and Lung Function of Patients with Chronic Obstructive Pulmonary Disease

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