Extracellular and intracellular actions of azadirachtin on the electrophysiological properties of cultured rat DRG neurones

Scott, Roderick H.; O’Brien, Kirsty; Roberts, Louise; Mordue, W.; Mordue, Jenny

doi:10.1016/s0742-8413(99)00014-6

Cited by 10 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results suggested that AZ generates its neurotoxcity by partially inhibiting calcium channels, which can decrease the excitability of the D. melanogaster CNS. By contrast, a previous study claimed that AZ has an inhibitory effect on the potassium channels in cultured dorsal root ganglion neurons . Instead of reducing the excitability of a neuron, Scott et al suggested that AZ could increase the excitability of mammal neurons.…”

Section: Discussionmentioning

confidence: 89%

“…By contrast, a previous study claimed that AZ has an inhibitory effect on the potassium channels in cultured dorsal root ganglion neurons. 5 Instead of reducing the excitability of a neuron, Scott et al suggested that AZ could increase the excitability of mammal neurons. This suggested that AZ may inhibit the CNS, but also excite the peripheral nervous system, and therefore have multiple targets in the nervous system.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Azadirachtin blocks the calcium channel and modulates the cholinergic miniature synaptic current in the central nervous system of Drosophila

Qiao

Zou

Lai

et al. 2013

Pest Management Science

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Azadirachtin blocks the calcium channel and modulates the cholinergic miniature synaptic current in the central nervous system of Drosophila

Qiao

Zou

Lai

et al. 2013

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…On the other hand, neem leaf is available throughout the year and has been chosen for experimentation for having its insecticidal property (Isman 1997). Azadirachtin, as a biopesticide, has minimal effect on non-target organisms such as natural enemies and pollinators (Lowery and Isman 1995;Naumann and Isman 1996) and is non-toxic to vertebrates (Scott et al 1999;Salehzadeh et al 2002). Azadirachtin acts in a dose-dependent way, i.e.…”

Section: Introductionmentioning

confidence: 99%

Effect of aqueous extract of Azadirachta indica A. Juss (neem) leaf on oocyte maturation, oviposition, reproductive potentials and embryonic development of a freshwater fish ectoparasite Argulus bengalensis Ramakrishna, 1951 (Crustacea: Branchiura)

2014

View full text Add to dashboard Cite

In present study, a microcosm experiment is carried out to investigate the efficacy of 120 and 250-ppm crude aqueous extract of Azadirachta leaf on oocyte maturation, oviposition, embryonic development and hatching of the eggs of a fish ectoparasite Argulus bengalensis. Relative abundance of different maturing oocyte stages in the ovary of the parasite from different age groups was enumerated, and marked variations were obtained. Significant depletion in the abundance of pre-vitollogenic, vitellogenic and post-vitellogenic oocytes was recorded, which indicates impairment in maturation. Chromatin condensation of the oocytes of treated parasite indicates apoptosis of oogenic cells. Strong oviposition deterrence was evident by the elevated oviposition deterrence index of 0.18 and 0.52 at respective toxin levels. The treated parasites invested less number of eggs per oviposition, and hatching percentage of the eggs reduced markedly. In vitro treatment of eggs within 70 min of incubation exhibited coagulation of yolk material and subsequent reduction in hatching percentage. However, treatment applied after this critical period, hatching was not significantly altered. In vitro treatment of eggs at 80 min of incubation resulted in normal development. It signifies that azadirachtin affects the early developmental events but not the later. Presumably, azadirachtin either affects early gene expression of the embryo or antagonizes any of the substances of the zygote required for sustaining early developmental process. The result of the present experiment suggests that azadirachtin could be a promising agent to control argulosis through inhibition of the reproductive maturity of the parasite as well as through interference of its embryonic development.

show abstract

“…In the case of azadirachtin, the antenna‐heart was more sensitive than the frontal ganglion of the stomatogastric nervous system of P. americana , which seems to be resistant to azadirachtin in the tested concentrations. Scott et al. (1999) demonstrated that azadirachtin has an inhibitory effect on the voltage‐dependent K + ‐channels in cultured rat neurones, resulting in a prolongation of the repolarization phase of the evoked action potentials.…”

Section: Discussionmentioning

confidence: 99%

“…However, the real physiological effects of azadirachtin are still not clear (Singh 2003;reviews: Koolman et al 1988;Schmutterer 1990;Mordue and Blackwell 1993). Scott et al (1999) have shown that azadirachtin causes an inhibition of voltage-dependent K + -channels in cultured rat neurones. Salehzadeh et al (2003) observed an antimitotic effect of azadirachtin on cultured insect cells, which indicates a possible long-term effect.…”

Section: Introductionmentioning

confidence: 99%

Physiological effects of the natural products quassin, cinnamaldehyde and azadirachtin on Periplaneta americana (L.)

Hertel

Müller

2006

J Applied Entomology

View full text Add to dashboard Cite

The action of the plant products quassin, cinnamaldehyde and azadirachtin was examined using pharmacological and electrophysiological methods. All three substances inhibited the beat rate of the antenna-heart, with quassin causing a diastolic and cinnamaldehyde a systolic block. Quassin delayed and azadirachtin at first accelerated the frequency of action potentials in the heart muscle, but 5 · 10 )3 m of the latter compound degraded the spike pattern and decreased the heart rate. A typical prolongation of the duration of the action potentials was observed. In recordings from a nerve of the frontal ganglion, quassin and cinnamaldehyde reduced the intervals between the spontaneous bursts, which turned into a continuous spike discharge with a decline in activity, while azadirachtin had no effect. Measurements in a single neurone of this ganglion revealed that quassin had a suppressive effect on the frequency and amplitude of the action potentials. These results suggest that the physiological effects demonstrated do not cause the primary insecticidal actions of these plant products.

show abstract

Extracellular and intracellular actions of azadirachtin on the electrophysiological properties of cultured rat DRG neurones

Cited by 10 publications

References 17 publications

Azadirachtin blocks the calcium channel and modulates the cholinergic miniature synaptic current in the central nervous system of Drosophila

Azadirachtin blocks the calcium channel and modulates the cholinergic miniature synaptic current in the central nervous system of Drosophila

Effect of aqueous extract of Azadirachta indica A. Juss (neem) leaf on oocyte maturation, oviposition, reproductive potentials and embryonic development of a freshwater fish ectoparasite Argulus bengalensis Ramakrishna, 1951 (Crustacea: Branchiura)

Physiological effects of the natural products quassin, cinnamaldehyde and azadirachtin on Periplaneta americana (L.)

Contact Info

Product

Resources

About