Extracellular calmodulin regulates growth and cAMP-mediated chemotaxis in Dictyostelium discoideum

O’Day, Danton H.; Huber, Robert J.; Suarez, Andres

doi:10.1016/j.bbrc.2012.07.147

Cited by 11 publications

(16 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The existence of extCaM in Dictyostelium was validated in subsequent studies on CyrA. Western blotting revealed that CaM is present in the extracellular medium during growth and treatment of growth phase cells with exogenous CaM inhibits cell proliferation [27,47]. While the presence of extCaM during growth was suspected, our analyses unexpectedly revealed that high and constant levels of extCaM are present during growth as well as during starvation, aggregation ( i.e.…”

Section: Current Researchsupporting

confidence: 51%

“…Earlier work showed that antagonizing CaM inhibited cAMP chemotaxis with several CaMBPs directly linked to the event [48]. These results were supported and extended when exogenous CaM was shown to enhance cAMP chemotaxis [47]. Ongoing and continued investigations into the CaMBP CyrA and its EGFL repeats reinforced the role of extCaM and extracellular CaMBPs in this event.…”

Section: Current Researchmentioning

confidence: 72%

“…, cAMP mediated-chemotaxis), multicellular tissue formation ( i.e. , slug formation) and later developmental stages [27,47]. The absence of major cytoplasmic proteins (e.g., tubulin) in the extracellular medium verified that this extCaM is due to secretion and not cell death [27].…”

Section: Current Researchmentioning

confidence: 99%

“…These events and the multiple functions of extCaM are summarized in Figure 4. When cells starve, as a prelude to multicellular development, extCaM comes into play for the developmental regulation of cAMP chemotaxis that drives cell aggregation [47]. Earlier work showed that antagonizing CaM inhibited cAMP chemotaxis with several CaMBPs directly linked to the event [48].…”

Section: Current Researchmentioning

confidence: 99%

“…ExtCaM shows a gradient of diffuse distribution in the front and middle of the slug, but forms punctate deposits in increasing numbers further back in the slug and into the ECM trail [47]. In contrast, CyrA is most concentrated in the sheath at the tip of the slug diminishing in amounts towards the rear [27].…”

Section: Current Researchmentioning

confidence: 99%

See 4 more Smart Citations

Matricellular Signal Transduction Involving Calmodulin in the Social Amoebozoan Dictyostelium

O’Day

Huber

2013

Genes

View full text Add to dashboard Cite

The social amoebozoan Dictyostelium discoideum undergoes a developmental sequence wherein an extracellular matrix (ECM) sheath surrounds a group of differentiating cells. This sheath is comprised of proteins and carbohydrates, like the ECM of mammalian tissues. One of the characterized ECM proteins is the cysteine-rich, EGF-like (EGFL) repeat-containing, calmodulin (CaM)-binding protein (CaMBP) CyrA. The first EGFL repeat of CyrA increases the rate of random cell motility and cyclic AMP-mediated chemotaxis. Processing of full-length CyrA (~63 kDa) releases two major EGFL repeat-containing fragments (~45 kDa and ~40 kDa) in an event that is developmentally regulated. Evidence for an EGFL repeat receptor also exists and downstream intracellular signaling pathways involving CaM, Ras, protein kinase A and vinculin B phosphorylation have been characterized. In total, these results identify CyrA as a true matricellular protein comparable in function to tenascin C and other matricellular proteins from mammalian cells. Insight into the regulation and processing of CyrA has also been revealed. CyrA is the first identified extracellular CaMBP in this eukaryotic microbe. In keeping with this, extracellular CaM (extCaM) has been shown to be present in the ECM sheath where it binds to CyrA and inhibits its cleavage to release the 45 kDa and 40 kDa EGFL repeat-containing fragments. The presence of extCaM and its role in regulating a matricellular protein during morphogenesis extends our understanding of CaM-mediated signal transduction in eukaryotes.

show abstract

Section: Current Researchsupporting

confidence: 51%

Section: Current Researchmentioning

confidence: 72%

Section: Current Researchmentioning

confidence: 99%

Section: Current Researchmentioning

confidence: 99%

Section: Current Researchmentioning

confidence: 99%

See 3 more Smart Citations

Matricellular Signal Transduction Involving Calmodulin in the Social Amoebozoan Dictyostelium

O’Day

Huber

2013

Genes

View full text Add to dashboard Cite

show abstract

Proteomic profiling of the extracellular matrix (slime sheath) of Dictyostelium discoideum

Huber

O’Day

2015

Proteomics

Self Cite

View full text Add to dashboard Cite

Dictyostelium discoideum has historically served as a model system for cell and developmental biology, but recently it has gained increasing attention as a model for the study of human diseases. The extracellular matrix (ECM) of this eukaryotic microbe serves multiple essential functions during development. It not only provides structural integrity to the moving multicellular pseudoplasmodium, or slug, it also provides components that regulate cell motility and differentiation. An LC/MS/MS analysis of slug ECM revealed the presence of a large number of proteins in two wild-type strains, NC4 and WS380B. GO annotation identified a large number of proteins involved in some form of binding (e.g. protein, polysaccharide, cellulose, carbohydrate, ATP, cAMP, ion, lipid, vitamin), as well as proteins that modulate metabolic processes, cell movement, and multicellular development. In addition, this proteomic analysis identified numerous expected (e.g. EcmA, EcmD, discoidin I, discoidin II), as well as unexpected (e.g. ribosomal and nuclear proteins) components. These topics are discussed in terms of the structure and function of the ECM during the development of this model amoebozoan and their relevance to ongoing biomedical research.

show abstract

Sphingosylphosphorylcholine alleviates hypoxia-caused apoptosis in cardiac myofibroblasts via CaM/p38/STAT3 pathway

Liu

Yang

et al. 2020

Apoptosis

View full text Add to dashboard Cite

Extracellular calmodulin regulates growth and cAMP-mediated chemotaxis in Dictyostelium discoideum

Cited by 11 publications

References 24 publications

Matricellular Signal Transduction Involving Calmodulin in the Social Amoebozoan Dictyostelium

Matricellular Signal Transduction Involving Calmodulin in the Social Amoebozoan Dictyostelium

Proteomic profiling of the extracellular matrix (slime sheath) of Dictyostelium discoideum

Sphingosylphosphorylcholine alleviates hypoxia-caused apoptosis in cardiac myofibroblasts via CaM/p38/STAT3 pathway

Contact Info

Product

Resources

About