Extracellular DNA: A Relevant Plant Damage-Associated Molecular Pattern (DAMP) for Crop Protection Against Pests—A Review

Ferrusquía-Jiménez, Noelia I.; Chandrakasan, Gobinath; Torres-Pacheco, Irineo; Rico-García, Enrique; Feregrino-Pérez, Ana A.; Guevara-González, Ramón Gerardo

doi:10.1007/s00344-020-10129-w

Cited by 27 publications

(31 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The plant immune response is triggered by the recognition of exogenous molecules called molecular patterns associated with pathogens or microbes (PAMP: Pathogen-Associated Molecular Pattern/MAMP: Microbe-Associated Molecular Pattern) through transmembrane receptor proteins called pattern recognition receptors (PRR), which are found on the surface of plant cells and are responsible for guiding immunity to microbial infection in all plant species ( Chisholm et al, 2006 ; Jones and Dangl, 2006 ; Bittel and Robatzek, 2007 ; Boller and Felix, 2009 ; Postel and Kemmerling, 2009 ; Bhat and Ryu, 2016 ; Choi and Klessig, 2016 ; Ferrusquía-Jiménez et al, 2020 ). PRR stimulation activates immunity triggered by PAMP (PTI: Pattern Triggered Immunity) ( Dodds and Rathjen, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

“…Since that discovery, relevant evidence of the immunogenic function of extracellular DNA (eDNA) as immunity activator has been reported in plants ( Barbero et al, 2016 ; Duran-Flores and Heil, 2018 ; Vega-Muñoz et al, 2018 ; Kawasaki, 2019 ). Based on this idea, on one hand, plants could recognize exogenous DNA from pathogen microorganisms and activate immunity (i.e., DNA acting as PAMP) ( Ferrusquía-Jiménez et al, 2020 ). On the other hand, fragments of nuclear or mitochondrial DNA molecules that appear in the extracellular or cytosolic compartment indicating cellular dysfunction or damage, including loss of integrity of nuclei, mitochondria, or whole cells, (i.e., DNA acting as DAMP) can also be recognized ( Vega-Muñoz et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Fragmented DNA From Plant Pathogens on the Protection Against Wilt and Root Rot of Capsicum annuum L. Plants

et al. 2021

View full text Add to dashboard Cite

Chili pepper (Capsicum annuum L.) production is affected by wilt and root rot, the most devastating disease caused by the pathogen complex of oomycete Phytophthora capsici Leon and the fungi Fusarium oxysporum Schlecht and Rhizoctonia solani Kühn, infecting roots, stems, leaves, and fruits. Fungicides are currently inefficient against this disease and have a high environmental impact. The use of elicitors is a sustainable alternative for inducing resistance to wilting and root rot. DNA fragments of an organism’s own origin (conspecific or self-DNA) have shown the ability to inhibit growth and activate defense mechanisms in some plant species. In this investigation, the effect of the fragmented DNA mixture of Phytophthora capsici L., Fusarium oxysporum S., and Rhizoctonia solani K. on the protection against wilt and root rot of Capsicum annuum L. plants was evaluated. Changes in plant performance, phenolics, and flavonoids contents, as well as gene expression involved in the production of defense metabolites after the fragmented and unfragmented DNA mixture in three concentrations (20, 60, and 100 μg mL–1) in chili peppers, were studied. The results obtained showed a decrease in plant height in 60 and 100 μg mL–1 concentrations in absence of pathogens. Moreover, the treatment with fragmented DNA 100 μg mL–1 showed significant increase in the content of phenolic compounds and total flavonoids as well as gene expression associated to plant defense in comparison with control plants. Interestingly, foliar application of DNA fragments of the pathogen complex to a concentration of 100 μg mL–1 caused a 40% decrease in the mortality of infected plants with the pathogens at 30 days post-inoculation compared with control plants inoculated with the pathogen complex but not sprayed with DNA fragments. These results suggested a perspective for application of fragmented DNA of these pathogens at the agricultural level in crop protection strategies to cope with wilt and root rot in Capsicum.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Fragmented DNA From Plant Pathogens on the Protection Against Wilt and Root Rot of Capsicum annuum L. Plants

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This observation is mainly based on the variety of drawbacks addressed by Brilli et al (2019) which still need to be further discussed and overcome in the future. However, new concepts exploring the potential of DAMPs as plant protective compounds found especially eDNA (Ferrusquía-Jiménez et al, 2020) to be a new candidate for application in the field. In addition to such treatments directly spraying compounds produced by wounded plant tissues on unwounded crops, we would like to focus onto damage-induced volatile compounds (DIVs).…”

Section: Resultsmentioning

confidence: 99%

“…With the increasing acceptance of the "damaged-self recognition" concept (Heil, 2009) for plants, the number of DAMPs, their putative reception and signaling and the corresponding literature continuously increased. Thus, here we avoid providing another collection of DAMPs and refer to recent reviews that give comprehensive overviews (Boller and Felix, 2009;Heil and Land, 2014;Gust et al, 2017;Quintana-Rodriguez et al, 2018;Hou et al, 2019;Ferrusquía-Jiménez et al, 2020). Nevertheless, some typical examples must be mentioned such as peptides, cell wall components, nucleic acid fragments, and extracellular ATP (eATP).…”

Section: Introductionmentioning

confidence: 99%

Plant–Plant Communication: Is There a Role for Volatile Damage-Associated Molecular Patterns?

Meents

Mithöfer

2020

Front. Plant Sci.

View full text Add to dashboard Cite

Damage-associated molecular patterns (DAMPs) are an ancient form of tissue-derived danger or alarm signals that initiate cellular signaling cascades, which often initiate defined defense responses. A DAMP can be any molecule that is usually not exposed to cells such as cell wall components, peptides, nucleic acid fragments, eATP and other compounds. DAMPs might be revealed upon tissue damage or during attack. Typically, DAMPs are derived from the injured organism. Almost all eukaryotes can generate and respond to DAMPs, including plants. Besides the molecules mentioned, certain volatile organic compounds (VOCs) can be considered as DAMPs. Due to their chemical nature, VOCs are supposed to act not only locally and systemically in the same plant but also between plants. Here, we focus on damage-induced volatiles (DIVs) that might be regarded as DAMPs; we will review their origin, chemical nature, physiochemical properties, biological relevance and putative function in plant-plant communications. Moreover, we discuss the possibility to use such airborne DAMPs as eco-friendly compounds to stimulate natural defenses in agriculture in order to avoid pesticides.

show abstract

“…Ferrusquía-Jiménez et al. [ 49 ] proposed the relevant role of extracellular DNA as a plant damage-associated molecular pattern (DAMP) for improving crop resistance towards pests. The mechanism involves: (i) inhibitory activity as the biologic control of the pests and/or (ii) elicitation activity to induce immune responses such as plant vaccines.…”

Section: Extracellular Dnamentioning

confidence: 99%

Green pesticide: Tapping to the promising roles of plant secreted small RNAs and responses towards extracellular DNA

Meitha

Esyanti

Iriawati

et al. 2021

Non-coding RNA Research

View full text Add to dashboard Cite

The diverse roles of non-coding RNA and DNA in cross-species communication is yet to be revealed. Once thought to only involve intra-specifically in regulating gene expression, the evidence that these genetic materials can also modulate gene expression between species that belong to different kingdoms is accumulating. Plants send small RNAs to the pathogen or parasite when they are being attacked, targeting essential mRNAs for infection or parasitism of the hosts. However, the same survival mechanism is also deployed by the pathogen or parasite to destabilize plant immune responses. In plants, it is suggested that exposure to extracellular self-DNA impedes growth, while to extracellular non-self-DNA induces the modulation of reactive oxygen species, expression of resistance related genes, epigenetic mechanism, or suppression of disease severity. Exploring the potential of secreted RNA and extracellular DNA as a green pesticide could be a promising alternative if we are to provide food for the future global population without further damaging the environment. Hence, some studies on plant secreted RNA and responses towards extracellular DNA are discussed in this review. The precise mode of action of entry and the following cascade of signaling once the plant cell is exposed to secreted RNA or extracellular DNA could be an interesting topic for future research.

show abstract

Extracellular DNA: A Relevant Plant Damage-Associated Molecular Pattern (DAMP) for Crop Protection Against Pests—A Review

Cited by 27 publications

References 101 publications

Effect of Fragmented DNA From Plant Pathogens on the Protection Against Wilt and Root Rot of Capsicum annuum L. Plants

Effect of Fragmented DNA From Plant Pathogens on the Protection Against Wilt and Root Rot of Capsicum annuum L. Plants

Plant–Plant Communication: Is There a Role for Volatile Damage-Associated Molecular Patterns?

Green pesticide: Tapping to the promising roles of plant secreted small RNAs and responses towards extracellular DNA

Contact Info

Product

Resources

About