Extracellular Matrix Components as Diagnostic Tools in Inflammatory Bowel Disease

Golusda, Laura; Kühl, Anja A.; Siegmund, Britta; Paclik, Daniela

doi:10.3390/biology10101024

Cited by 9 publications

(10 citation statements)

References 101 publications

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“…ECM remodeling is increasingly recognized as a key step in the progression of disease and is a potential therapeutic target for IBD ( 9 , 60 , 61 ). Mounting evidence points to the increased activity of fecal ( 19 , 22 , 62 ) and, specifically, bacterial proteases ( 63 , 64 ) associated with disease severity in UC.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory Bowel Disease-Associated Gut Commensals Degrade Components of the Extracellular Matrix

Porras,

Zhou,

Shi

et al. 2022

mBio

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Section: Discussionmentioning

confidence: 99%

Inflammatory Bowel Disease-Associated Gut Commensals Degrade Components of the Extracellular Matrix

Porras,

Zhou,

Shi

et al. 2022

mBio

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“…First, degradation of components of the basement membrane like collagen IV and laminin could further disrupt epithelial integrity (6, 67). Second, degradation of submucosal ECM can precipitate the recruitment and activation of immune cells (52, 68). For example, cleavage of hyaluronic acid (69, 70) and collagen (8) triggers the recruitment of nearby leukocytes.…”

Section: Discussionmentioning

confidence: 99%

“…ECM remodeling is increasingly recognized as a key step in the progression of disease and a potential therapeutic target for IBD (9, 52, 53). Mounting evidence points to increased activity of fecal (19, 22, 54) and, specifically, bacterial proteases (55, 56) associated with disease severity in UC.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory bowel disease-associated gut commensals degrade components of the extracellular matrix

Porras

Zhou

Shi

et al. 2022

Preprint

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Extracellular matrix (ECM) remodeling has emerged as a key feature of inflammatory bowel disease (IBD), and ECM fragments have been proposed as markers of clinical disease severity. Recent studies report increased protease activity in the gut microbiota of IBD patients. Nonetheless, the relationship between gut microbiota and ECM remodeling has remained unexplored. We hypothesized that members of the human gut microbiome can degrade host ECM, and that bacteria-driven remodeling, in turn, can enhance colonic inflammation. Through a variety of in vitro assays, we first confirmed that multiple bacterial species found in the human gut are capable of degrading specific ECM components. Clinical stool samples obtained from ulcerative colitis patients also exhibited higher levels of proteolytic activity in vitro compared to those of their healthy counterparts. Furthermore, culture supernatants from bacteria species capable of degrading human ECM accelerated inflammation in a dextran sodium sulfate (DSS)-induced colitis. Finally, we identified several of the bacterial proteases and carbohydrate degrading enzymes (CAZymes) potentially responsible for ECM degradation in vitro. Some of these protease families and CAZymes were also found in increased abundance in a metagenomic cohort of IBD. These results demonstrate that some commensal bacteria in the gut are indeed capable of degrading components of human ECM in vitro and suggest this proteolytic activity may be involved in the progression of IBD. A better understanding of the relationship between nonpathogenic gut microbes, host ECM, and inflammation could be crucial to unravel some of the mechanisms underlying host-bacteria interactions in IBD and beyond.

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“…ECM is a highly dynamic acellular network composed of collagen, fibronectin, and several other proteins (79). It is of great importance in the inflammatory process.…”

Section: Ecm Degradationmentioning

confidence: 99%

Malassezia in Inflammatory Bowel Disease: Accomplice of Evoking Tumorigenesis

Yang

Ouyang

et al. 2022

Front. Immunol.

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Accumulating evidence indicates that patients with inflammatory bowel disease (IBD) have a significantly higher risk of developing different cancers, while the exact mechanism involved is not yet fully understood. Malassezia is a lipid-dependent opportunistic yeast, which colonizes on mammalian skin and internal organs. Also, dysbiosis in fungal communities accompanied by high level of Malassezia are fairly common in inflammatory diseases such as IBD and various cancers. In cancer patients, higher levels of Malassezia are associated with worse prognosis. Once it is ablated in tumor-bearing mice, their prognostic conditions will be improved. Moreover, Malassezia manifests multiple proinflammatory biological properties, such as destruction of epithelial barrier, enrichment of inflammatory factors, and degradation of extracellular matrix (ECM), all of which have been reported to contribute to tumor initiation and malignant progression. Based on these facts, we hypothesize that high levels of Malassezia together with mycobiome dysbiosis in patients with IBD, would aggravate the microecological imbalance, worsen the inflammatory response, and further promote tumorigenesis and deterioration. Herein, we will discuss the detrimental properties of Malassezia and explore the key role of this fungus in the correlation between IBD and cancer, in order to take early surveillance and intervention to minimize the cancer risk in individuals with IBD.

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Extracellular Matrix Components as Diagnostic Tools in Inflammatory Bowel Disease

Cited by 9 publications

References 101 publications

Inflammatory Bowel Disease-Associated Gut Commensals Degrade Components of the Extracellular Matrix

Inflammatory Bowel Disease-Associated Gut Commensals Degrade Components of the Extracellular Matrix

Inflammatory bowel disease-associated gut commensals degrade components of the extracellular matrix

Malassezia in Inflammatory Bowel Disease: Accomplice of Evoking Tumorigenesis

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