Extracellular matrix molecules and their possible roles in the regeneration of frog nervous system

Matesz, Clara; Módis, László; Halasi, Gabor; Szigeti, Zsuzsa M.; Felszeghy, Szabolcs; Bácskai, Tímea; Székely, György

doi:10.1016/j.brainresbull.2005.06.014

Cited by 23 publications

(20 citation statements)

References 16 publications

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“…Therefore, the aim of this study was to describe the distribution pattern of HA in the frog CNS by using a highly specific histochemical probe. Preliminary results have been published by Matesz et al (2005).…”

Section: Indexing Terms: Extracellular Matrix; Hyaluronic Acid; Visuamentioning

confidence: 93%

“…The blocks were fixed by immersion in SainteMarie's fixative (99% absolute ethanol and 1% glacial acetic acid) overnight (Sainte-Marie, 1962;Tuckett et al, 1988). This alcohol-based fixative was selected to minimize the loss of HA, which is a water-soluble polysaccharide, and we also obtained good results when using the Sainte-Marie's fixative in histochemistry of HA (Felszeghy et al, 2000;Matesz et al, 2005). The blocks were embedded in paraffin, and sections were cut with a Reichert microtome at a thickness of 10 m. After deparaffination, the sections were treated with 2% hydrogen peroxide in phosphate-buffered saline (PBS), pH 7.4, for 30 minutes and with 1% bovine serum albumin (BSA) in PBS for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

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Distribution of hyaluronan in the central nervous system of the frog

et al. 2006

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The qualitative and quantitative distribution pattern of hyaluronan (HA), a component of the extracellular matrix (ECM), was studied in the frog central nervous system by using a highly specific HA probe and digital image analysis. HA reaction was observed in both the white and the gray matter, showing a very intense staining around the perikarya and dendrites in the perineuronal net (PN). In the telencephalon, strong reaction was found in different parts of the olfactory system, in the pallium, and in the amygdala. In the diencephalon, intensive staining was found in the nucleus of Bellonci, the dorsal habenula, the lateral and central thalamic nuclei, and the subependymal zone of the third ventricle. In the mesencephalon, layers of optic tectum displayed different intensities, with the strongest reaction in layers B, D, F, 3, and 5. Other structures of the mesencephalon showed regional differences. The PN was especially intensively stained around the perikarya of the toral nuclei, the oculomotor and trochlear nuclei, and the basal optic nucleus. In the rhombencephalon, the granular layer of cerebellum, the vestibulocochlear nuclei, the superior olive, the spinal tract of the trigeminal nerve, and parts of the reticular formation showed the most intense reaction in the PN. In the spinal cord, considerable HA staining was found in the white matter and around the perikarya of motoneurons. The present study is the first description of the HA-positive areas of frog brain and spinal cord demonstrating the heterogeneity of HA distribution in the frog central nervous system.

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Section: Indexing Terms: Extracellular Matrix; Hyaluronic Acid; Visuamentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Distribution of hyaluronan in the central nervous system of the frog

et al. 2006

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“…For the detection of HA (Tammi et al 1989(Tammi et al , 1994Matesz et al 2005;Szigeti et al 2006) a specific biotinylated hyaluronan binding probe (bHABP) (kindly provided by R. Tammi and M. Tammi, Department of Anatomy University of Kuopio, Kuopio, Finland) was applied. The sections were incubated overnight in a final dilution of 1:10 (5 lg/ml PBS) at 4°C, and the signal was subsequently visualized with the avidin/biotin (ABC) staining kit (Vector Laboratories, Burlingame, CA, USA) and diaminobenzidine (DAB, 0.5 mg/ml in PBS) according to the manufacturer's instructions.…”

Section: Surgical and Histological Proceduresmentioning

confidence: 99%

“…An intriguing ECM macromolecule found both in the peripheral and central nervous system is hyaluronan (HA). It is ubiquitously present in many mammalian species (Margolis et al 1975;Delpech et al 1989;Eggli et al 1992;Gong et al 1994;Yasuhara et al 1994) and in the frog (Matesz et al 2005;Szigeti et al 2006) showing regional differences in the brain and spinal cord.…”

Section: Introductionmentioning

confidence: 98%

“…Another distinguished site of the specific localization of HA is the so-called perineuronal net (PN), which is a condensation of ECM macromolecules around the perikaryon, proximal dendrites and the axon hillock (Celio et al 1998;Fox and Caterson 2002;Matesz et al 2005). It is supposed that HA plays a crucial role in the maintenance of the complex architecture of the PN establishing different molecular interactions.…”

Section: Introductionmentioning

confidence: 99%

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The effect of vestibular nerve section on the expression of the hyaluronan in the frog, Rana esculenta

et al. 2007

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Following postganglionic lesion of the eighth cranial nerve, the changes in the expression of hyaluronan (HA), one of the extracellular matrix macromolecules, were examined in the medial (MVN) and lateral (LVN) vestibular nuclei and in the entry or transitional zone (TZ) of the nerve in the frog. HA was detected in different survival times by using a specific biotinylated hyaluronan-binding probe. HA expression was defined by the area-integrated optical density (AIOD), calculated from pixel intensities of digitally captured images. During the first postoperative days the perineuronal net (PN), a HA-rich area around the neurons, was not distinguishable from the surrounding neuropil in the MVN and LVN, characterized by a bilateral drop of AIOD specifically on the operated side. From postoperative day 14 onwards AIOD increased whilst the PN reorganized. In contrast, the AIOD wobbled up and down bilaterally without any trend in the TZ. Statistical analysis indicated that AIOD changes in the structures studied ran parallel bilaterally presumably because of the operation. Our results demonstrated for the first time that (1) the lesion of the eighth cranial nerve is accompanied by the modification of AIOD reflected HA expression in the MVN, LVN and TZ, (2) different tendencies exist in the time course of AIOD in the structures studied and (3) these tendencies are similar on the intact and operated sides. Our findings may suggest an area dependent molecular mechanism of HA in the restoration of vestibular function.

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Dynamics of perineuronal nets over amphibian metamorphosis

Edwards

Risch

Hoke

2020

J of Comparative Neurology

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Extracellular matrix materials known as perineuronal nets (PNNs) have been shown to have remarkable consequences for the maturation of neural circuits and stabilization of behavior. It has been proposed that, due to the possibly long-lived biochemical nature of their components, PNNs may be an important substrate by which longterm memories are stored in the central nervous system. However, little empirical evidence exists that shows that PNNs are themselves stable once established. Thus, the question of their temporal dynamics remains unresolved. We leverage the dramatic morphological and behavioral transformations that occur during amphibian metamorphosis to show that PNNs can be highly dynamic in nature. We used established lectin histochemistry to show that PNNs undergo drastic reconstruction during the metamorphic transition. Pre-metamorphic tadpoles have abundant lectinlabeled pericellular material, which we interpret to be PNNs, surrounding neurons throughout the central nervous system. During the metamorphic transition, these structures degrade, and begin to reform in the months following metamorphosis. We show that PNN sizes and staining intensity further change over metamorphosis, suggesting compositional rearrangement. We found PNNs in brain regions with putative homology to regions in mammals with known PNN function, and in other shared

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Extracellular matrix molecules and their possible roles in the regeneration of frog nervous system

Cited by 23 publications

References 16 publications

Distribution of hyaluronan in the central nervous system of the frog

Distribution of hyaluronan in the central nervous system of the frog

The effect of vestibular nerve section on the expression of the hyaluronan in the frog, Rana esculenta

Dynamics of perineuronal nets over amphibian metamorphosis

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