Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering

Colazzo, Francesca; Sarathchandra, Padmini; Smolenski, Ryszard T.; Chester, Adrian H.; Tseng, Yuan-Tsan; Czernuszka, Jan T.; Yacoub, Magdi H.; Taylor, Patricia M.

doi:10.1016/j.biomaterials.2010.09.003

Cited by 85 publications

(61 citation statements)

References 23 publications

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“…Apart from sensitivity to chemical stimuli, BMSCs and ASCs were also found to be sensitive to mechanical stimuli (12)(13)(14). Numerous studies have shown that mechanical stimuli play an important role in bone tissue engineering, which is also referred to as functional bone tissue engineering (15,16).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of the osteogenic differentiation capacity of human bone marrow- and human adipose-derived stem cells under cyclic tensile stretch using quantitative analysis

Ye¹,

Du²,

Guo³

et al. 2012

International Journal of Molecular Medicine

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Abstract. Studies comparing the osteogenic differentiation capacity of human bone marrow-derived stem cells (hBMSCs) and human adipose-derived stem cells (hASCs) cultured in osteogenic differentiation medium have been inconclusive. Apart from chemical stimuli, mechanical stimuli have also been shown to be important in bone tissue engineering, which is referred to as functional bone tissue engineering. hBMSCs and hASCs have been shown to be sensitive to both chemical and mechanical stimuli. In an attempt to find a better seed cell in functional bone tissue engineering, we tried to quantify the osteogenic differentiation capacity of hBMSCs and hASCs under both mechanical and chemical stimuli. In this study, hBMSCs and hASCs were isolated from the same volunteers. Cells were cultured in osteogenic differentiation medium with and without exposure to cyclic tensile stretch (CTS). Quantitative measurement of alkaline phosphatase (ALP) activity revealed that the osteogenic differentiation capacity of hBMSCs was similar to that of hASCs in the early phase of differentiation in the CTS-stimulated groups. Quantitative measurement of mineralization showed that the late-phase osteogenic differentiation capacity of the hBMSCs was superior to that of hASCs in the CTS-stimulated groups. Reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed 5 and 10 days after cell culture. The results of the RT-PCR revealed that the osteogenic differentiation capacity of hASCs was inferior to that of hBMSCs both in the CTS-stimulated and unstimulated groups. All the results showed that both hBMSCs and hASCs were sensitive to CTS during the osteogenic differentiation process. This study compared the osteogenic differentiation capacity of hBMSCs and hASCs in response to mechanical stimulations and has important implications for the use of stem cells in functional bone tissue engineering and regenerative medicine.

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Section: Introductionmentioning

confidence: 99%

Comparative study of the osteogenic differentiation capacity of human bone marrow- and human adipose-derived stem cells under cyclic tensile stretch using quantitative analysis

Ye¹,

Du²,

Guo³

et al. 2012

International Journal of Molecular Medicine

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“…It was demonstrated that stretching increased the incorporation of hydroxyproline in ADSC, which was followed by the enhanced production of collagen and elastin crosslinks. This observation represents a fundamental mechanism which is essential to maintain the load capacity of leaflets and allows therewith valve functionality [44]. In the present study, after four weeks bioreactor cultivation, TEHV based on ADSC consisted on average of 29 µg collagen (HYP) per mg dry tissue, which is 65% of the values of native valves.…”

Section: Discussionmentioning

confidence: 65%

Adipose Derived Tissue Engineered Heart Valve

Frese

Sanders

Beer³

et al. 2015

J Tissue Sci Eng

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“…Colazzo et al recently reported that adipose-derived progenitor cells could sense mechanical stimulation and produce elastin when the substrate was stretched. 40 It is possible that cell differentiation will be further enhanced by proper mechanical stimulation in our system as well.…”

Section: Discussionmentioning

confidence: 99%

Artificial Niche Combining Elastomeric Substrate and Platelets Guides Vascular Differentiation of Bone Marrow Mononuclear Cells

Allen

Gao

et al. 2011

Tissue Engineering Part A

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Bone marrow-derived progenitor cells are promising cell sources for vascular tissue engineering. However, conventional bone marrow mesenchymal stem cell expansion and induction strategies require plating on tissue culture plastic, a stiff substrate that may itself influence cell differentiation. Direct scaffold seeding avoids plating on plastic; to the best of our knowledge, there is no report of any scaffold that induces the differentiation of bone marrow mononuclear cells (BMNCs) to vascular cells in vitro. In this study, we hypothesize that an elastomeric scaffold with adsorbed plasma proteins and platelets will induce differentiation of BMNCs to vascular cells and promote vascular tissue formation by combining soft tissue mechanical properties with platelet-mediated tissue repairing signals. To test our hypothesis, we directly seeded rat primary BMNCs in four types of scaffolds: poly(lactide-co-glycolide), elastomeric poly(glycerol sebacate) (PGS), platelet-poor plasma-coated PGS, and PGS coated by plasma supplemented with platelets. After 21 days of culture, osteochondral differentiation of cells in poly(lactide-co-glycolide) was detected, but most of the adhered cells on the surface of all PGS scaffolds expressed calponin-I and a-smooth muscle actin, suggesting smooth muscle differentiation. Cells in PGS scaffolds also produced significant amount of collagen and elastin. Further, plasma coating improves seeding efficiency, and platelet increases proliferation, the number of differentiated cells, and extracellular matrix content. Thus, the artificial niche composed of platelets, plasma, and PGS is promising for artery tissue engineering using BMNCs.

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Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering

Cited by 85 publications

References 23 publications

Comparative study of the osteogenic differentiation capacity of human bone marrow- and human adipose-derived stem cells under cyclic tensile stretch using quantitative analysis

Comparative study of the osteogenic differentiation capacity of human bone marrow- and human adipose-derived stem cells under cyclic tensile stretch using quantitative analysis

Adipose Derived Tissue Engineered Heart Valve

Artificial Niche Combining Elastomeric Substrate and Platelets Guides Vascular Differentiation of Bone Marrow Mononuclear Cells

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