Extracellular matrix proteins involved in pseudoislets formation

Maillard, Élisa; Sencier, Marie-Christine; Langlois, A.; Bietiger, W.; Krafft, MP; Pinget, M.; Sigrist, S.

doi:10.4161/isl.1.3.9754

Cited by 15 publications

(9 citation statements)

References 35 publications

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“…β-cells in PIs show improved function as measured by increased insulin production and improved glucose-stimulated insulin secretion (GSIS) [4], [5], [6], [7], [8], [9], [10]. These effects are mediated in part by the formation of a 3-D configuration which enhances β-cell – cell contact [5], [9], increases calcium signaling [11], and preserves extracellular matrix (ECM) proteins [12]. Despite their usefulness, PI generation requires extensive cell handling and may take several days to form (7–14 d).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

et al. 2013

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β cell pseudoislets (PIs) are used for the in vitro study of β-cells in a three-dimensional (3-D) configuration. Current methods of PI induction require unique culture conditions and extensive mechanical manipulations. Here we report a novel co-culture system consisting of high passage β-cells and islet-derived endothelial cells (iECs) that results in a rapid and spontaneous formation of free-floating PIs. PI structures were formed as early as 72 h following co-culture setup and were preserved for more than 14 d. These PIs, composed solely of β-cells, were similar in size to that of native islets and showed an increased percentage of proinsulin-positive cells, increased insulin gene expression in response to glucose stimulation, and restored glucose-stimulated insulin secretion when compared to β-cells cultured as monolayers. Key extracellular matrix proteins that were absent in β-cells cultured alone were deposited by iECs on PIs and were found in and around the PIs. iEC-induced PIs are a readily available tool for examining β cell function in a native 3-D configuration and can be used for examining β-cell/iEC interactions in vitro.

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Section: Introductionmentioning

confidence: 99%

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

et al. 2013

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“…13,14 These β-cell line pseudoislets show much improved insulin secretory characteristics compared to equivalent cells maintained as monolayers, demonstrating the importance of homotypic β-cell to β-cell interactions in the generation and maintenance of appropriate insulin secretory responses, and validating the pseudoislet model for in vitro studies of the regulation of insulin secretion. Since the first publication of the characteristics of MIN6 pseudoislets in 1999, 13 several other laboratories have adopted cell line-derived pseudoislet models for research, [15][16][17][18][19] but this model is still not widely used despite the advantages of widespread availability, simplicity and cost-effectiveness. The Diabetes Research Group at King's College London recently (April 2010) hosted a symposium with the aims of disseminating knowledge about the pseudoislet model and highlighting emerging research themes from current studies using pseudoislets.…”

Section: What Islet Alternatives Are Available For Research?mentioning

confidence: 99%

Pseudoislets as primary islet replacements for research

Persaud¹,

Arden²,

Bergsten³

et al. 2010

Islets

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“…Of the self‐assembled PA nanomatrices, our results exhibited the formation of β‐cell clusters on PA‐YIGSR and PA‐RGDS, which also correlated to greater glucose‐stimulated insulin secretion. Improved responses after 7 d would be explained by the creation of native ECM within the β‐cell clusters, which would provide improved cell–cell interactions and create a more favorable microenvironment for the function of MIN6 cells . The greater glucose‐stimulated insulin secretion of the PA‐YIGSR and PA‐RGDS indicates that the PAs are capable of creating pancreatic ECM mimics, consequently providing benefit to MIN6 cell survival, viability, and function.…”

Section: Resultsmentioning

confidence: 99%

Improved MIN6 β‐Cell Function on Self‐Assembled Peptide Amphiphile Nanomatrix Inscribed with Extracellular Matrix‐Derived Cell Adhesive Ligands

Lim

Antipenko

Vines

et al. 2013

Macromolecular Bioscience

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Understanding the role of the pancreatic extracellular matrix (ECM) in supporting islet survival and function drives the pursuit to create biomaterials that imitate and restore the pancreatic ECM microenvironment. To create an ECM mimic holding bioinductive cues for β-cells, self-assembled peptide amphiphiles (PAs) inscribed with four selected ECM-derived cell adhesive ligands are synthesized. After 7 days, compared to control groups cultured on biologically inert substrates, MIN6 β-cells cultured on PAs functionalized with YIGSR and RGDS cell adhesive ligands exhibit elevated insulin secretion in responses to glucose and also form β-cell clusters. These findings suggest that the self-assembled PA nanomatrix may be utilized to improve pancreatic islet transplantation for treating type 1 diabetes.

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Extracellular matrix proteins involved in pseudoislets formation

Cited by 15 publications

References 35 publications

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

In Vitro Formation of β Cell Pseudoislets Using Islet-Derived Endothelial Cells

Pseudoislets as primary islet replacements for research

Improved MIN6 β‐Cell Function on Self‐Assembled Peptide Amphiphile Nanomatrix Inscribed with Extracellular Matrix‐Derived Cell Adhesive Ligands

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