Extracellular vesicles from human umbilical cord mesenchymal stem cells reduce lipopolysaccharide-induced spinal cord injury neuronal apoptosis by mediating miR-29b-3p/PTEN

Targeted Delivery of RGD-CD146+CD271+ Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Promotes Blood–Spinal Cord Barrier Repair after Spinal Cord Injury

“…Among these molecules, exosome-loaded microRNAs play a crucial part in regulating biological processes. 46 In this study, deep microRNA sequencing was employed to investigate the underlying biological mechanisms responsible for the effects of CD146 + CD271 + UCMSC-Exos on BSCB permeability, leading to the identification of 32 differentially expressed microRNAs (Figure 5A). Subsequently, the expression levels of 16 significantly upregulated micro-RNAs were validated using qRT-PCR.…”

“…Exosomes encompass a diverse array of components, including microRNAs, mRNA, and proteins, which can be transferred to target cells through intercellular contact. Among these molecules, exosome-loaded microRNAs play a crucial part in regulating biological processes . In this study, deep microRNA sequencing was employed to investigate the underlying biological mechanisms responsible for the effects of CD146 + CD271 + UCMSC-Exos on BSCB permeability, leading to the identification of 32 differentially expressed microRNAs (Figure A).…”

“…The extraction method of the exosomes was as previously described: collect cell supernatant and sequentially centrifuge at 500, 2000, 10 000, and 100 000 g to acquire sediment. Resuspend the sediment in PBS . We used transmission electron microscopy (TEM) to examine the morphology of exosomes.…”

“…Resuspend the sediment in PBS. 46 We used transmission electron microscopy (TEM) to examine the morphology of exosomes. Additionally, the size and concentration of the exosomes were assessed by utilizing a flow nanoanalyzer instrument.…”

Mesenchymal stem cell therapy for ischemic stroke: Novel insight into the crosstalk with immune cells

“…Studies in experimental models mimicking ischemic stroke imply that MSCs can abrogate ischemia-induced neuronal survival and neurological function recovery. As such, under such conditions, MSCs derived from bone marrow, adipose tissue, and umbilical cord can significantly reduce neuronal death ( 29 – 31 ). In addition, neurological recovery is also associated with the successful restitution of vascular and glial functions.…”