Extracellular Vesicles from Human Urine-Derived Stem Cells Ameliorate Particulate Polyethylene-Induced Osteolysis

Human urine-derived stem cell exosomes delivered via injectable GelMA templated hydrogel accelerate bone regeneration

“…Further, a subpopulation of urothelial cells was identified that expressed stem cell markers and had multifunctional differentiation potential [ 35 , 53 ]. Our group successfully demonstrated in the previous study that USC EXOs have the physiological function of promoting osteogenesis and inhibiting osteolysis [ 10 , 11 ]. Nevertheless, the poor retention of USC EXOs in vivo may restrict other biological effects and clinical promotion.…”

“…Extraction and purification of exosomes from human urine-derived stem cells were referenced from previous studies [ 10 , 11 ]. Briefly, upon 70–80% confluence of the human urine-derived stem cells (passage 3) in the 25 ​T culture flask, replace the medium depotted exosome and incubate for 48 ​h.…”

“…We have previously demonstrated that human urine-derived stem cell exosomes (human USC EXOs) have multifunctional differentiation potential under in vitro culture conditions. These exosomes have also been shown to suppress osteolysis and promote osteogenesis [ 10 , 11 ].…”

Stem Cells and Bone Tissue Engineering

“…Li H et al, reported that human UDSC-derived extracellular vesicles (UDSC-EVs) exhibited a cup-like morphology with a double-layered membrane structure, which was positive for CD63 and TSG101 and negative for calnexin. In vitro, UDSC-EVs promoted the osteogenic differentiation of BMMSCs and reduced proinflammatory factor production, as well as osteoclastic activity, in RAW264.7 macrophage cells [ 157 ]. In vivo, local injection of UDSC-EVs around the central sites of the calvaria of ultra-high-molecular-weight polyethylene (UHMWPE) particle-induced osteolysis decreased inflammatory cytokine generation and osteolysis compared with the control groups and significantly increased bone formation [ 157 ].…”

Urine-derived stem cells: Promising advancements and applications in regenerative medicine and beyond

“…Moreover, in vitro, USCs display a robust proliferative capacity, comparable to that of bone marrow-derived mesenchymal stem cells (MSCs), while both have demonstrated similar effectiveness in cartilage repair in vivo [ 66 ]. USCs and their exosomes have demonstrated therapeutic potential in mitigating various bone-related ailments, such as osteoporosis, bone fractures, intervertebral disc degeneration, osteonecrosis of the femoral head (ONFH), and expediting rotator cuff healing [ [69] , [70] , [71] , [72] , [73] , [74] , [75] , [76] , [77] ].…”