Extract of neem (<i>Azadirachta indica</i>) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry

Ali, Edris; Islam, Md. Sadequl; Hossen, Md. Ismail; Khatun, Mst. Minara

doi:10.1002/vms3.511

Cited by 22 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A study done by Altayb et al [ 23 ] demonstrated that the methanolic extract of A. indica contains various bioactive compounds, such as beta-D-mannopyranoside and O-geranyl, which are capable of inhibiting the growth of various bacterial strains. Raghavendra et al [ 24 ] studied the antifungal and antimicrobial activity of the neem, and the findings demonstrated superior results when compared with 3% sodium hypochlorite (NaOCl). The acetone extracts of neem were more effective at inhibiting bacterial growth compared to aqueous extracts, which indicates the influence of solvents against bacteria [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Formulation of Neem and Echinacea Gel for Oral Health Along With the Evaluation of Antimicrobial, Cytotoxic, Anti-inflammatory, and Free Radical Scavenging Activity: An In Vitro Study

Sindhusha,

Rajasekar

2024

Cureus

View full text Add to dashboard Cite

Background Herbs have been used in medical practice for centuries and continue to play a significant role in modern complementary and alternative medicine. Phytochemicals in these herbs possess strong antioxidant and anti-inflammatory properties, which are beneficial in targeting oral health issues, such as dental plaque, gingivitis, and oral microbial infections. As research progresses, the challenge remains to translate these natural compounds into safe, effective, and accessible treatments for a wide range of diseases. Aim The aim of this research was to formulate the neem and echinacea gel along with the evaluation of antimicrobial, anti-inflammatory, free-radical scavenging activity, and cytotoxic potential. Materials and methods The neem and echinacea gel was prepared using a concentrated powdered mixture of neem and echinacea (5 grams each) to which 100 ml of distilled water was added, and the mixture was boiled for 30 minutes at 60°C. The 10 ml concentrate was mixed with 20 ml of a carbopol and carboxymethyl cellulose (CMC) mixture and mixed thoroughly, which resulted in neem and echinacea gel. Then, the antimicrobial, anti-inflammatory, cytotoxic potential, and free-radical scavenging activity of the gel were evaluated. The data obtained were statistically analyzed with the help of a paired t-test, where a p-value of less than 0.05 was considered statistically significant. Results The antimicrobial assay showed that neem and echinacea gel at the concentration of 100 micrograms showed a greater zone of inhibition against S taphylococcus aureus (3.15 ± 0.26), S treptococcus mutans (2.48 ± 0.45), E nterococcus faecalis (2.89 ± 0.15), and Candida albicans (4.28 ± 0.87). The cytotoxic test revealed that even at an 80 µg concentration of the extract, more than 70% of the nauplii were vital, which indicated that the gel was not cytotoxic. The highest anti-inflammatory activity (78.39 ± 1.82) of the gel was seen at 50 micrograms when compared with diclofenac sodium (73.16 ± 1.80). The free radical scavenging activity showed that the 2,2-diphenyl-1-picrylhydrazyl (DPPH) absorbance of the neem and echinacea extract was highest at 50 micrograms. Conclusion The combination of neem and echinacea extract-based gel possessed high antimicrobial and anti-inflammatory activity when compared with standard drugs, such as amoxicillin and diclofenac sodium. The antioxidant activity of the gel was equal to butylated hydroxytoluene (BHT), and also the gel has a low cytotoxic potential even at its higher concentrations. Hence, the gel can be used as a natural remedy with minimal side effects, making it a valuable alternative to chemical agents.

show abstract

Section: Discussionmentioning

confidence: 99%

Formulation of Neem and Echinacea Gel for Oral Health Along With the Evaluation of Antimicrobial, Cytotoxic, Anti-inflammatory, and Free Radical Scavenging Activity: An In Vitro Study

Sindhusha,

Rajasekar

2024

Cureus

View full text Add to dashboard Cite

show abstract

“…They also possess the characteristics of anti-inammation, antibacterial, antifungal, antimalarial, antidiabetic, and antiviral. 20,21 Literature study revealed the presence of multiple phytochemicals in the leaves extract of the neem plant. The most common of these components are alkaloids, avonoids, tannins, steroids, and saponins.…”

Section: Phytochemicals In Azadirachta Indicamentioning

confidence: 99%

Neem plant extract-assisted synthesis of CeO₂nanoparticles for photocatalytic degradation of piroxicam and naproxen

Quddus,

Shah,

Nisar

et al. 2023

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…Alkaloids, terpenoids, saponins, flavonoids, phenols, tannins, steroids, and aldehydes Ali et al, 2021 [103] Bangladesh…”

Section: Leaves Extractmentioning

confidence: 99%

Phytochemical composition and antibacterial activities of Azadirachta indica (Neem): significance of traditional medicine in combating infectious diseases and antimicrobial resistance

Mudenda,

Banda,

Mohamed

et al. 2023

J Pharmacogn Phytochem

View full text Add to dashboard Cite

Traditional medicines such as Azadirachta indica (A. indica) possess medicinal properties that should be explored in drug discovery to combat infectious diseases and antimicrobial resistance. This study provided insights into the antibacterial activities of A. indica. This narrative review utilised Google Scholar and PubMed to search for articles that were published from January 2000 to September 2023 on the antibacterial activities of A. indica. The study found that A. indica has activity against Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, and other bacteria. The antibacterial activities of A. indica were reported to be due to its phytochemical content such as phenolics, tannins, saponins, flavonoids, terpenoids, fatty acids, and alkaloids. This review found that A. indica has antimicrobial activity against some Gram-negative and Gram-positive bacteria due to the presence of phytochemicals. There is a need to increase investment in drug discovery and the integration of traditional medicine into the healthcare system.

show abstract

Extract of neem (Azadirachta indica) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry

Cited by 22 publications

References 27 publications

Formulation of Neem and Echinacea Gel for Oral Health Along With the Evaluation of Antimicrobial, Cytotoxic, Anti-inflammatory, and Free Radical Scavenging Activity: An In Vitro Study

Formulation of Neem and Echinacea Gel for Oral Health Along With the Evaluation of Antimicrobial, Cytotoxic, Anti-inflammatory, and Free Radical Scavenging Activity: An In Vitro Study

Neem plant extract-assisted synthesis of CeO₂nanoparticles for photocatalytic degradation of piroxicam and naproxen

Phytochemical composition and antibacterial activities of Azadirachta indica (Neem): significance of traditional medicine in combating infectious diseases and antimicrobial resistance

Contact Info

Product

Resources

About

Extract of neem (Azadirachta indica) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry

Cited by 22 publications

References 27 publications

Formulation of Neem and Echinacea Gel for Oral Health Along With the Evaluation of Antimicrobial, Cytotoxic, Anti-inflammatory, and Free Radical Scavenging Activity: An In Vitro Study

Formulation of Neem and Echinacea Gel for Oral Health Along With the Evaluation of Antimicrobial, Cytotoxic, Anti-inflammatory, and Free Radical Scavenging Activity: An In Vitro Study

Neem plant extract-assisted synthesis of CeO2nanoparticles for photocatalytic degradation of piroxicam and naproxen

Phytochemical composition and antibacterial activities of Azadirachta indica (Neem): significance of traditional medicine in combating infectious diseases and antimicrobial resistance

Contact Info

Product

Resources

About

Neem plant extract-assisted synthesis of CeO₂nanoparticles for photocatalytic degradation of piroxicam and naproxen