Extraction and analysis of bioactive compound from freshwater crab tissue

Abstract

The adult species of freshwater crabs (Berytephusa Cuniclaris) were collected from the Northern region of Maharashtra, India, and isolated, refrigerated in the laboratory. The living crabs were euthanized at -30ºC by thermal shock for 15 minutes. The crabs were then clearly dissented and the tissue was extracted from the crab's abdomen, the crude extract obtained from their tissue. The crude extract was completely dried off in the microwave and pulverized then a cold maceration process was used by immersing the powder into the ethyl alcohol for maintaining the stability of the bioactive compounds at low temperature and vacuum-packed for 21 days at room temperature. Stirring was given infrequently due to raising up the speed of extraction of the bioactive compound diffuses from the cell in anticipation of equilibrium reached. The repetitive filtration was done to refine the compound and concentrated with rotator evaporators to eliminate the excess amount of ethanol which form a pure product. The current study is described the first time in India with the help of an advanced analytical tool like UV, FTIR, NMR, LCMS, Single-crystal analysis, and antimicrobial potencies that the crab is beneficial to human as well as a medicinal source due to the presence of bioactive compounds like cortisol, aldosterone, isoleucine, and estradiol with the most isolated compounds dexamethasone, testosterone, and norethisterone have existed in the cluster form.