Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

Jiang, Bin; Feng, Zhibiao; Liu, Chunhong; Xu, Ying; Li, Dongmei; Ji, Guo

doi:10.1007/s13197-014-1319-5

Cited by 40 publications

(29 citation statements)

References 26 publications

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“…The few studies reported on esterase purification by ATPS are focused on esterases from plant origin. Jiang, Feng, Liu, Xu, Li and Ji [50] explored the purification of wheat-esterase using ionic liquids-based ATPSs. Under optimum conditions (20% (w/w) of 1-Butyl-3-methylimidazolium tetrafluoroborate and 25% (w/w) of NaH 2 PO 4 pH 4.8), the enzyme was obtained with a purification factor of 4.2 and a recovery yield of 89%.…”

Section: Partial Purification Of Esterasementioning

confidence: 99%

Integrated strategy for purification of esterase from Aureobasidium pullulans

Lemes

Silvério

Rodrigues

et al. 2019

Separation and Purification Technology

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Esterases catalyze the cleavage and formation of ester bonds of a broad range of substrates presenting a widespread spectrum of industrial applications. This work aimed to partially purify and characterize an esterase from Aureobasidium pullulans LABIOTEC 01 produced in a culture medium containing olive mill wastewater. Esterase purification was evaluated using different strategies, namely the enzyme recovery by PEG-salt aqueous twophase systems (ATPSs); and the enzyme precipitation with ammonium sulfate, acetone, and ethanol. The best purification factor (18 ± 2) was obtained when the ATPS composed of 20% (w/w) polyethylene glycol (PEG) 6000 and 5.8% (w/w) potassium phosphate buffer (PPB) pH 8.0 was combined with acetone precipitation. The partially purified enzyme presented an optimum pH of 5.0, although it remained active in the pH range of 4.5 to 7.5 (≥ 50% relative activity). The optimum temperature was found to be 60°C. Furthermore, the addition of salts such as FeCl 3 , CuSO 4 and MnCl 2 promoted an increase in the enzymatic activity (above 100%). The enzyme was found to be stable and showed high activity when exposed to polar solvents such as dimethyl sulfoxide, dimethylformamide, and methanol. The use of an integrated strategy of purification combining simple purification methods such as ATPS and precipitation was herein reported for the first time for esterase. This strategy proved to be an interesting approach to partially purify the esterase produced under submerged fermentation by A. pullulans. Furthermore, the enzyme showed potential to be applied in industrial biocatalytic processes using high temperature and different salts or solvents. Also, the production of esterase using olive mill wastewater as substrate demonstrated to be a suitable alternative to reduce and valorize agro-industrial residues.

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Section: Partial Purification Of Esterasementioning

confidence: 99%

Integrated strategy for purification of esterase from Aureobasidium pullulans

Lemes

Silvério

Rodrigues

et al. 2019

Separation and Purification Technology

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“…100% recovery of Bovine serum albumin is reported using phosphonium and ammonium-based ILs [22]. 1-Butyl-3-methylimidazolium tetrafluoroborate based ILs enabled the preferential partitioning of wheat esterase from wheat extracts towards ILs rich phase with a recovery of 88.93% [23].The practical advantage of tailoring the ionic liquids enabled even for the separation of enantiomers of aminoacids from racemic mixture [24]. In a study to understand the driving forces influencing extraction by ionic liquids, electrostatic interaction between surface charged residues of aminoacids and positive charge of cation of ionic liquid is determined to be the most prominent [25].…”

Section: G Ionic Liquids In Atps Based Extractionmentioning

confidence: 99%

A Review on Aqueous Two Phase Systems with a Special Mention on Application of Ionic Liquids

Antonisamy¹

2019

IJRASET

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Aqueous two phase system (ATPS) is an excellent biocompatible extraction methodology having wide applications in the purification of various biomolecules. In this method water comprises almost 80-85 % in both phases and hence the biomolecules are highly stable during extraction. ATPS system offers the advantage of process integration and there by increases the yield of biomolecules. Optimization of different parameters like type and concentration of phase forming compounds, addition of external agents like salts, temperature, physicochemical nature of biomolecules are influential in determining the partitioning of biomolecules towards a phase. In this review the methodology of ATPS and its application, challenges and future prospects are discussed in detail with a special mention on application of ionic liquids in ATPS.

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“…The aqueous biphasic system (ABS) is recognized as an environmentally friendly method, as an alternative to the traditional LLE, since both phases of the ABS are aqueous solutions, and it could be used for organic compounds [1][2][3][4][5] and biological macromolecule [6][7][8][9] extractions without using any volatile organic solvent. Up to now, ABS could be composed of polymer and salt, polymer and polymer, ionic liquid (IL) and polymer, IL and salt, IL and surfactant.…”

Section: Introductionmentioning

confidence: 99%

“…Among these factors, the effect of pH value of salt solution on the ABS formation should not be ignored. Its influence on the protein extraction by ABSs [7,9,[23][24][25][26][27][28][29][30] was fully investigated, since the pH value could affect the electrostatic interaction and hydrophobic interaction in the target proteins extraction, and then influence their extraction recoveries. However, its effect on the formation of ABS by cholinium-based IL and salt was scarcely studied [22], since it is relatively difficult to create the cholinium IL-based ABSs by salts.…”

Section: Introductionmentioning

confidence: 99%

Extraction of bisphenol A in honey samples using aqueous biphasic systems coupled with high‐performance liquid chromatography

Tian

Bai

2018

Separation Science Plus

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An aqueous biphasic system based on cholinium propanoate and phosphate solution was developed for the fast extraction and determination of bisphenol A in honey samples. The phase diagrams of the aqueous biphasic systems with cholinium propanoate (ionic liquid) and phosphate solutions were investigated, and the biphasic regions of the aqueous biphasic systems decreased with the increase of pH values of the phosphate solutions. The aqueous biphasic system was applied for the analyte extraction. After extraction, the analyte was separated on a C18 column (150 mm × 2.1 mm i. d., 5 μm) using acetonitrile and ammonium acetate as the mobile phase. The parameters, such as the conductivity and viscosity of the ionic liquid solutions, pH values of the phosphate solutions, and extraction efficiency were optimized to achieve the excellent extraction performance. Good linearity was shown for the analyte over the tested concentration range (0.01–10.0 mg/kg) with correlation coefficient higher than 0.991. Three spiked levels (0.01, 0.05, 0.10 mg/kg) were applied for determination of the recoveries of the analyte in honey samples in the range of 78–112% with relative standard deviations of 5.5–10.2%. The limit of detection and limit of quantification for the analyte were 0.003 and 0.01 mg/kg, respectively. The developed method was applied for the determination of the analyte in real samples with high sensitivity.

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Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

Cited by 40 publications

References 26 publications

Integrated strategy for purification of esterase from Aureobasidium pullulans

Integrated strategy for purification of esterase from Aureobasidium pullulans

A Review on Aqueous Two Phase Systems with a Special Mention on Application of Ionic Liquids

Extraction of bisphenol A in honey samples using aqueous biphasic systems coupled with high‐performance liquid chromatography

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