Extraction, characterization of bioactive compounds and biological activities of the leaves of Podocarpus lambertii Klotzch ex Endl

Bandeira, Débora Marina; Corrêa, Juliana Moço; Laskoski, Larissa Valéria; Batista, Joelma Marques; Rosset, Jéssica; Costa, Willian Ferreira da; Kuo, L H; Pinto, Fabiana Gisele da Silva

doi:10.1016/j.jarmap.2022.100427

Cited by 7 publications

(10 citation statements)

References 58 publications

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“…The identification of the compounds present in the essential oil was performed with a FOCUS GS gas chromatograph (Thermo Electron) coupled to a DSQ II mass spectrometer, a detector with impact electronic ionization at 70 V and a quadrupole mass analyzer. For chromatographic separation, a DB-5 fused silica capillary column (30 m x 0.25 mm i.d., 0.25 μm film thickness) and stationary phase of 5% phenyl / 95% dimethylpolysiloxane were used, according to the methodology of Bandeira et al (2022).…”

Section: Chemical Analysis Of Myrcia Glomerata Essential Oil -Gas Chr...mentioning

confidence: 99%

Analysis of the chemical constituents of Myrcia glomerata G.Burton & E.Lucas leaves and assessment of their antimicrobial, antioxidant, and antibiofilm potential against pathogenic microorganisms

Batista,

Bandeira,

Laskoski

et al. 2024

Cad. Pedagógico

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The Myrtaceae family plays a significant role in the Angiosperm landscape in Brazil, standing out as one of the most relevant. Within this context, the genus Myrcia ranks as the fourth largest, and its plant extracts and essential oils from these species are recognized as a valuable source of plant bioactives. In this context, Myrcia glomerata, popularly known as guamirim, stands out. Despite belonging to the Myrtaceae family, there is a lack of detailed research on its biological activities and chemical composition. The phytochemical investigation of the different plant extracts: ethanolic (EE), methanolic (ME), hexane (HE), acetone (AE), ethyl acetate (EAE) and dichloromethane (DE) revealed the presence of saponins, free steroids, alkaloids, anthocyanins, anthocyanidins, flavones, flavonols, xanthones, chalcones, aurones, flavononols and tannins, leading to the quantification of phenolic compounds (flavonoids, tannins, and alkaloids). The essential oil of the leaves, identified by gas chromatography coupled to mass spectrometry (GC-MS) identified 29 compounds, corresponding to 97.54% of the total area of the essential oil of M. glomerata. The main compounds found were sesquiterpenes, Elemicin (76.85%), Elixene (3.99%), E-caryophyllene (2.25%). EE showed the best antimicrobial potential, with concentrations ranging from 0.39 to 25 mg.mL-1, with Staphylococcus aureus and Proteus mirabilis strains being more susceptible in EE, ME and AE, showing 98.72%, 96.23% and 88.60% antioxidant activity, respectively. As for the antibiofilm potential, ME showed the greatest biomass reduction (90.53%) for Salmonella enterica Typhimurium, with cell viability of 9.02%. These results demonstrate the potential of EE and ME extracts from M. glomerata, representing a possible source of natural bioactives to be used in pharmaceuticals for the control of resistant microorganisms.

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Section: Chemical Analysis Of Myrcia Glomerata Essential Oil -Gas Chr...mentioning

confidence: 99%

Analysis of the chemical constituents of Myrcia glomerata G.Burton & E.Lucas leaves and assessment of their antimicrobial, antioxidant, and antibiofilm potential against pathogenic microorganisms

Batista,

Bandeira,

Laskoski

et al. 2024

Cad. Pedagógico

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“…After drying, 150 µl of 95% ethanol was added and left in contact for 30 minutes, then the contents of the wells were transferred to a new plate and the optical density reading (OD) was performed at 570 nm using Biotek microplate reader, Epoch model. The data were calculated in general mean OD of biofilm biomass quantified by staining using crystal violet (Christensen et al, 1985;Bandeira et al, 2022). The percentage of eradication of biofilm was determined by the equation: Percentage of eradication (%): ((OD negative control -OD experimental) / OD negative control) X 100 in the Microsoft Excel 2010 program.…”

Section: Quantification Of Biofilm Biomass By Crystal Violetmentioning

confidence: 99%

Phytochemical prospection and evaluation of antimicrobial, antioxidant and antibiofilm activities of extracts and essential oil from leaves of Myrsine umbellata Mart. (Primulaceae)

et al. 2022

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The species Myrsine umbellata is a native plant of Brazil, whose barks are traditionally used in herbal medicine to treat liver disorders and combat leprosy. Therefore, the aim of the study was to identify the phytochemical prospection of ethanolic (EE) and acetonic (EA) extracts by colorimetric tests and by gas chromatography coupled to mass spectrometry (GC-MS) of the essential oil (EO) of M. umbellata leaves; evaluate the antimicrobial activity in front of standard ATCC strains by the broth microdilution technique; the antioxidant potential by DPPH reduction method and antibiofilm action by crystal violet assay and cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) based on optical density. Phytochemical prospection of EE and EA detected the presence of free steroids, alkaloids, flavonoids (flavones, flavononoids, flavonols and xanthons) and tannins in both extracts (EE and EA) and saponins only in EE. In EO, the majority compounds identified were elixene, caryophyllene (E), spatulenol, d-Cadinene and aromadendrene. EA showed antimicrobial activity with MIC and MBC/MFC values ranging from 3.12 to 100 mg.mL-1, highlighting its efficiency on the Gram-positive strain S. epidermidis. EE showed antimicrobial potential in the range of 3.12 to 200 mg.mL-1, and the Gram-negative E. coli strain was the most susceptible. However, OE showed bacteriostatic potential against S. Typhimurium, S. Abaetetuba, P. aeruginosa, and S. epidermidis strains. The ability to sequester free radicals was evident in EA extract with antioxidant activity of 89.55% and in EE with 63.05%. The antibiofilm potential was observed in EE extract which eradicated the mature biofilm biomass of all tested bacteria with high activity (50% to 84.28%) and EO also showed antibiofilm effect on mature biofilm of UEL enteroaggregative E. coli, S. aureus and S. Enteritidis strains with biomass reduction percentage of 63.74%, 68.04% and 86.19%, respectively. These results indicate the potential of M. umbellata extracts and as a source of plant bioactivity for the development of new alternative strategies for the control of planktonic or biofilm-resistant microorganisms.

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“…Currently, they have been gaining great importance in research for the development of drugs of natural origin. Several groups of researchers have studied the biological activity of medicinal plants originating in various parts of the world, guided by the popular use of native species (SILVA FILHO et al, 2009;SANTOS et al, 2021;BANDEIRA et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Research with C. longa proved its antimicrobial activity (MAIA et al, 2003;KALAYCIOGLU et al, 2017), anti-inflammatory, antioxidant, antiprotozoal, anticarcinogenic andantifungal (AKRAM et al, 2010;MARCHI et al, 2016). Faced with the resistance that pathogenic microorganisms have developed, there is a need and encouragement for the search for drugs of natural origin (BANDEIRA et al, 2022). The bacterium E. coli, for example, is a commensal microorganism, present in the intestines of mammals and birds, which has been pointed out as one of the most frequent bacterial agents in diarrhea of humans and animals, and can cause, in humans, several infections, including meningitis (FERREIRA; KNÖBL, 2009;ALMEIDA, 2013).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of Curcuma longa L. on Escherichia coli

Bandeira¹,

Marchi²,

Gomes³

et al. 2023

Revista Científica Multidisciplinar Núcleo do Conhecimento

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Curcuma longa is a plant that has stood out in research evaluating antimicrobial, anti-inflammatory, antiprotozoal, anticarcinogenic and antifungal activity. The discovery of new alternatives for the development of drugs originating from active principles of plants is necessary considering the high adaptive capacity of different microorganisms. Thus, the present study aimed to evaluate the antimicrobial activity of extracts of oleoresin and powder suspension of rhizomes of Curcuma longa against the bacterium Escherichia coli. In this study, no antimicrobial activity was observed for the extracts of turmeric powder and oleoresin against Escherichia coli. There is a wide variety of results obtained in the literature in the face tests of C. longa on the different microorganisms, some positive, demonstrating antimicrobial action, and others negative. This divergence is justified by factors such as: different methodologies used, climate, soil types, fertilization, water availability of the plant’s cultivation, as well as the form of harvest and storage, which interfere in the components present and in their concentration in the plant. Although the results of this study were negative, it does not mean that the plant does not have antimicrobial activity, being indicated the continuity in the assays, aiming to identify the factors that generate disagreement between the studies already carried out.

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Extraction, characterization of bioactive compounds and biological activities of the leaves of Podocarpus lambertii Klotzch ex Endl

Cited by 7 publications

References 58 publications

Analysis of the chemical constituents of Myrcia glomerata G.Burton & E.Lucas leaves and assessment of their antimicrobial, antioxidant, and antibiofilm potential against pathogenic microorganisms

Analysis of the chemical constituents of Myrcia glomerata G.Burton & E.Lucas leaves and assessment of their antimicrobial, antioxidant, and antibiofilm potential against pathogenic microorganisms

Phytochemical prospection and evaluation of antimicrobial, antioxidant and antibiofilm activities of extracts and essential oil from leaves of Myrsine umbellata Mart. (Primulaceae)

Antimicrobial activity of Curcuma longa L. on Escherichia coli

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