Extraction, Isolation and Characterisation of Free and Bound Polyphenols From Neem (Azadirachta Indica Juss) Seed

Abstract

Free and bound polyphenols were extracted from raw and deffated neem seed and then characterized inorder to determine the effect of defatting on the quantity of polyphenols in neem seeds. The total phenolic content, total flavonoid content, total condensed tannin content and DPPH free radical scavenging activities (FRSA) of the raw and defatted neem seed were evaluated. The characterization of the polyphenols was ca rried out using gas chromatography. The chromatogram revealed the presence of a substantial amount of polyphenols in neem seed; the raw neem seed had higher contents of tannic acid, azidiradione, nimbin, nimbdin, salamin andazadirachtin. The DPPH free radical scavenging activities and quercetin content of the defatted neem seed were retained while other bioactive components were reduced.