Extraction, isolation and evaluation of anti-toxic principles from Moringa oleifera (MOF6) and Myristica fragrans (Trimyristin) upregulated Acetylcholinesterase concentrations in Sodium arsenite-induced neurotoxicity in rats.

Abstract

This study evaluated the neuroprotective effects of MOF6 (isolated from Moringa oleifera leaves) and Trimyristin (isolated from Myristica fragrans seeds) on Acetylcholinesterase concentrations in cerebral cortices of rats with Sodium arsenite-induced neurotoxicity. Sixty-five adult male rats (150 g-250 g) were randomly divided into thirteen groups comprising of five rats per group. Groups 1 and 3 received physiological saline and 1 ml/200 g bodyweight of Olive oil respectively for 9 weeks. Group 2 received 20 mg/kg bodyweight of Sodium arsenite (SA) for 6 weeks and left untreated for another 3 weeks. Groups 4-5 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 5.0 and 7.5 mg/kg bodyweight of MOF6 respectively for 6 weeks. Groups 6-7 received 20 mg/kg bodyweight of SA for 3 weeks followed by treatments with 15 and 30 mg/kg bodyweight of Trimyristin respectively for 6 weeks. Groups 8-11 received 5.0 and 7.5 mg/kg bodyweight of MOF6; 15 and 30 mg/kg bodyweight of Trimyristin respectively for 9 weeks. Groups 12-13 received 7.5 mg/kg bodyweight of MOF6 and 30 mg/kg bodyweight of Trimyristin respectively for 6 weeks followed by co-administration of each extract dose with 20 mg/kg bodyweight of SA for another 3 weeks. Histological examination of cerebral cortices and biochemical analyses of Acetylcholinesterase concentrations were carried out in all rats. Computed data were analyzed using Microsoft Excel 2016 with statistical significance at p≤0.05. Histo-pathological evaluations revealed normal histo-architecture of cerebral cortices of all rats. Results showed statistically significant (p≤0.05) increases in Acetylcholinesterase concentrations in rats of Groups 1-10 and 12 compared with Group 2 (2.78±1.76 𝜇mole/min/g). 7.5 mg/kg bodyweight of MOF6 showed the best therapeutic and neuro-regenerative potential against SA-induced neurotoxicity.Conclusions: Our findings implied that MOF6 and Trimyristin reversed downregulation of Acetylcholinesterase concentrations in SA-induced neurotoxicity in rats; and possess neuro-protective and neuro-regenerative potentials.

Anticancer potentials of morinda lucida and annona muricata on ki67 and multidrug resistance1 genes expressions in sodium arsenite-induced hepato-toxicity in rats

“…The number of rats employed in this study was determined based on the guidelines and approval of the University Ethical Review Committee (UERC) of University of Ilorin, Nigeria. The dose of Sodium Arsenite was determined from a previous study 17 , while the doses of ML and AM were determined from previous study on anticancer potentials of ML and AM in Lead acetate-induced toxicity in rats 18 . In addition, bodyweights (g) of all rats were measured on Day 1 of experimental procedure and at the end of each week.…”

“…Enzyme Linked Immunosorbent Assay (ELISA) of Concentrations of Ki67 and Multidrug Resistance1 (MDR1) Genes in Liver Tissues of Rats Liver parts were cut from each rat and placed in 10% formalin for histo-pathological examinations, processed for light microscopy using conventional histological procedures and obtained slides were stained with Hematoxyline and Eosin 17 . In addition, separate liver tissues were isolated and then subjected to thorough homogenization using porcelain mortar and pestle in ice-cold 0.25M sucrose, in the proportion of 1g to 4ml of 0.25M sucrose solution.…”

Cadmium Chloride-induced skin ulceration and inflammation in rats: Anti-inflammatory potentials of Moringa oleifera and Musa sapientium via NF-KB/IL-4/1L-10-mediated pathway.

“…MOF6 showed significant antioxidant and neuro-protective potentials against Cuprizone-induced cerebellar damage in rats (Omotoso et al, 2018). MOF6 equally showed neuro-protective potentials against dysregulated Acetylcholinesterase concentrations in Sodium arsenite-induced neurotoxicity in rats (Akinlolu et al, 2020a). In addition, MOF6 showed hepatoprotective, anti-proliferation and anti-drug resistance potentials in 7,12-Dimethylbenz(a)anthracene-induced hepatotoxicity in rats (Akinlolu et al, 2021).…”

In vivo anti-inflammatory effects of anti-toxic principles from Moringa oleifera(MO11) and Musa sapientum (MS06) via NF-KB/IL-4/IL-10- mediated pathway in Cadmium Chloride-induced hepato-toxicity.

“…MOF6 equally showed neuroprotective potentials against dysregulated Acetylcholinesterase concentrations in Sodium arsenite-induced neurotoxicity in rats (Akinlolu et al, 2020a). In addition, MOF6 possessed hepato-protective, antidrug resistance and anti-proliferation potentials in 7,12-Dimethylbenz(a)anthracene-induced hepatotoxicity in rats (Akinlolu et al, 2021).…”