Extraction, isolation and in vitro evaluation of affinisine from Tabernaemontana catharinensis in human melanoma cells

Rosales, Pauline Fagundes; Gower, Adriana E.; Benitez, Martha Lucia Ruiz; Pacheco, Bruna Silveira; Segatto, Natália Vieira; Roesch‐Ely, Mariana; Collares, Tiago; Seixas, Fabiana Kömmling; Moura, Sidnei

doi:10.1016/j.bioorg.2019.103079

Cited by 10 publications

(7 citation statements)

References 30 publications

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“…Sanguinaria canadensis extracts exhibited highly cytotoxic activity compared to previously published investigations of various plant extracts cytotoxicity against different melanoma cells [26][27][28][29][30][31][32][33][34][35][36]. IC 50 values obtained after exposure of A375 cells to Sanguinaria canadensis extracts were from 4.09 to 10.96 µg/mL, values after exposure of G-361 cells to the plant extract were from 1.22 to 1.60 µg/mL, and after exposure of SK-MEL-3 to the same extracts were from 0.88 to 1.36 µg/mL.…”

Section: Comparison Of In Vitro Cytotoxic Activity Of Sanguinaria Canadensis Extracts With Cytotoxic Activity Of Various Plant Extractsmentioning

confidence: 95%

“…Our results demonstrated that Sanguinaria canadensis extracts are cytotoxic to various human melanoma cell lines, with IC 50 values ranging from 0.88 to 10.96 µg/mL. Interestingly, the cytotoxicity was greater than that of other plant extracts containing various cytotoxic compounds, including alkaloids [26][27][28][29][30][31][32][33][34][35][36].…”

Section: Investigation Of In Vitro Cytotoxic Activity Of Sanguinaria Canadensis Extractsmentioning

confidence: 98%

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Determination of Cytotoxic Activity of Sanguinaria canadensis Extracts against Human Melanoma Cells and Comparison of Their Cytotoxicity with Cytotoxicity of Some Anticancer Drugs

et al. 2021

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Melanoma is an enormous global health burden, and should be effectively addressed with better therapeutic strategies. Therefore, new therapeutic agents are needed for the management of this disease. The aim of this study was the investigation of cytotoxic activity of some isoquinoline alkaloid standards and extracts obtained from Sanguinaria canadensis—collected before, during, and after flowering—against three different human melanoma cells (A375, G361, SK-MEL-3). The cytotoxicity of these extracts was not previously tested on these melanoma cell lines. Determination of alkaloid contents was performed by HPLC-DAD using Polar RP column and mobile phase containing acetonitrile, water, and 1-butyl-3-methylimidazolium tetrafluoroborate. The cytotoxicity of alkaloid standards was investigated by determination of cell viability and calculation of IC50 values. Significant differences were observed in the alkaloids content and cytotoxic activity of the extracts, depending on the season of collection of the plant material. In the Sanguinaria canadensis extracts high contents of sanguinarine (from 4.8543 to 9.5899 mg/g of dry plant material) and chelerythrine (from 42.7224 to 6.8722 mg/g of dry plant material) were found. For both of these alkaloids, very high cytotoxic activity against the tested cell lines were observed. The IC50 values were in the range of 0.11–0.54 µg/mL for sanguinarine and 0.14 to 0.46 µg/mL for chelerythrine. IC50 values obtained for Sanguinaria canadensis extracts against all tested cell lines were also very low (from 0.88 to 10.96 µg/mL). Cytotoxic activity of alkaloid standards and Sanguinaria canadensis extracts were compared with the cytotoxicity of anticancer drugs—etoposide, cisplatin, and hydroxyurea. In all cases except the one obtained for cisplatin against A375, which was similar to that obtained for Sanguinaria canadensis after flowering against the same cell line, IC50 values obtained for anticancer drugs were higher than the IC50 values obtained for sanguinarine, chelerythrine, and Sanguinaria canadensis extracts. Our results showed that Sanguinaria canadensis extracts and isoquinoline alkaloids, especially sanguinarine and chelerythrine, could be recommended for further in vivo experiments in order to confirm the possibility of their application in the treatment of human melanomas.

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Section: Comparison Of In Vitro Cytotoxic Activity Of Sanguinaria Canadensis Extracts With Cytotoxic Activity Of Various Plant Extractsmentioning

confidence: 95%

Section: Investigation Of In Vitro Cytotoxic Activity Of Sanguinaria Canadensis Extractsmentioning

confidence: 98%

Determination of Cytotoxic Activity of Sanguinaria canadensis Extracts against Human Melanoma Cells and Comparison of Their Cytotoxicity with Cytotoxicity of Some Anticancer Drugs

et al. 2021

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“…Tradicionalmente compostos como temozolomida, cisplatina e dacarbazina são os agentes quimioterápicos empregados no tratamento da doença, contudo, devido aos efeitos adversos causados pelas medicações, os autores [19] optaram por avaliar os efeitos do biocomponente afinisina, isolado da Tabernaemontana catharinensis A. DC. na proliferação celular e na apoptose de diferentes linhagens de melanoma (WM1366, SK-MEL-28 e A375), sendo também avaliado em uma linhagem de pele não-tumoral (CCD-1059Sk).…”

Section: Espécieunclassified

“…Conforme procedimento experimental descrito por Rosales et al [19] , do mesmo grupo de pesquisa, as inflorescências foram removidas dos caules que, em seguida, foram secos em estufa com circulação de ar forçada a 30°C durante sete dias. O material seco foi moído em moinho de facas e, posteriormente foi extraído por ultrassom utilizando etanol (10 mL.g -1 ) como solvente, com o equipamento configurado a 40% de amplitude, a uma potência de 500 W, durante 30 minutos.…”

Section: Espécieunclassified

Bioprospecção de plantas medicinais com potencial anticancerígeno no Brasil: caracterização e métodos de extração

Assumpção

Silva

Mendes

2022

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O câncer é um problema de saúde pública mundial e pesquisas têm apontado que o número de novos casos deve atingir 29,4 milhões até 2040. Além disso, o avanço desigual da doença causará maior impacto nos países de baixa e média renda. Nesse contexto, o avanço da doença associado aos efeitos adversos dos tratamentos empregados atualmente revela a urgência de avaliar métodos alternativos para combater o câncer. Com isso, o uso de plantas medicinais é desejável, por apresentarem menores contraindicações, graças ao baixo efeito antiproliferativo em tecidos saudáveis e elevada inibição para diversas linhagens tumorais. Sob a perspectiva brasileira, a bioprospecção de espécies vegetais é promissora, devido à elevada biodiversidade nacional. Com isso, o objetivo do presente trabalho foi de avaliar as vertentes da pesquisa na área de extração de compostos bioativos a partir de fontes vegetais, identificando as espécies, perfil fitoquímico e técnicas de extração mais utilizadas. Pode-se concluir que o uso de solventes orgânicos é massivo, o que prejudica o "scale-up" dos processos e pode inviabilizar o uso efetivo de fitoterápicos de plantas medicinais em escala industrial.

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“…This poor prognosis is mainly due to resistance to the conventional chemotherapy (4). A375 represents a well-established and characterized melanoma cell line belonging to a relatively undifferentiated and highly metastatic class of melanocytic cells characterized by epithelioid shape and lack of pigmentation (5,6). HU2 cells like human dermal fibroblasts (HDFs) belong to a dermis cell type with a mesenchymal origin and are found in all connective tissues.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Taxol in Combination with Vincristine and Vinblastine Against A375 Cell Line

Zandi

2021

Gene Cell Tissue

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Background: Annually, various types of cancer cause thousands of deaths globally, and identifying an appropriate therapeutic option for these disorders is of crucial importance. Side effects of anticancer drugs can be reduced through the promising strategy of combination therapy. Objectives: The present paper has investigated the in vitro cytotoxicity of Taxol, carboplatin, vinblastine, and vincristine alone and in combination against human malignant melanoma A375 cells and non-cancerous fibroblast HU2 cells to examine the possible side effects of the drugs. Methods: The cells were subjected to the examined compounds for 48 h, and the MTT test was conducted to evaluate the cytotoxicity. Results: The results indicated that the most significant effect was related to 120 μg/mL vincristine and 7.5 μg/mL Taxol+ vincristine treatments, with the survival amounts of 24 ± 0.6 and 28 ± 0%, respectively. In addition, the best 50% inhibitory effect was found to be related to Taxol + vincristine, vinblastine, and Taxol+ vinblastine treatments at the concentrations of 0.04, 2.2, and 3.4 μg/mL, respectively. Conclusions: According to the findings of in vitro toxicity, the evaluated complexes are not cytotoxic against human fibroblast HU2 cells. Also, the most significant effect on A375 cells was associated with vincristine treatment. No synergistic reaction was recorded among the different combinations of drugs based on the calculated CI values.

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Extraction, isolation and in vitro evaluation of affinisine from Tabernaemontana catharinensis in human melanoma cells

Cited by 10 publications

References 30 publications

Determination of Cytotoxic Activity of Sanguinaria canadensis Extracts against Human Melanoma Cells and Comparison of Their Cytotoxicity with Cytotoxicity of Some Anticancer Drugs

Determination of Cytotoxic Activity of Sanguinaria canadensis Extracts against Human Melanoma Cells and Comparison of Their Cytotoxicity with Cytotoxicity of Some Anticancer Drugs

Bioprospecção de plantas medicinais com potencial anticancerígeno no Brasil: caracterização e métodos de extração

Cytotoxicity of Taxol in Combination with Vincristine and Vinblastine Against A375 Cell Line

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