Extraction of Ascorbate Oxidase from Cucurbita maxima by Continuous Process in Perforated Rotating Disc Contactor Using Aqueous Two-Phase Systems

Abstract

The ascorbate oxidase is the enzyme used to determine the content of ascorbic acid in the pharmaceutical and food industries and clinics analyses. The techniques currently used for the purification of this enzyme raise its production cost. Thus, the development of alternative processes and with the potential to reduce costs is interesting. The application of aqueous two-phase system is proposed as an alternative to purification because it enables good separation of biomolecules. The objective of this study was to determine the conditions to continuously pre-purify the enzyme ascorbate oxidase by an aqueous two-phase system (PEG/citrate) using rotating column provided with perforated discs. Under the best conditions (20,000 g/mol PEG molar mass, 10% PEG concentration, and 25% citrate concentration), the system showed satisfactory results (partition coefficient, 3.35; separation efficiency, 54.98%; and purification factor, 1.46) and proved suitable for the pre-purification of ascorbate oxidase in continuous process.

Extraction of fibrinolytic proteases from Streptomyces sp. DPUA1576 using PEG-phosphate aqueous two-phase systems

“…The purification factor obtained in this study is also in agreement with other works in the literature reporting the purification of proteins from biological extracts, as is the case of bromelain (P = 1.81, using PEG/phosphate system) [34], alkaline protease (P = 1.80, using PEG/citrate system) [44] and xylose reductase (P = 1.1 using PEG/phosphate system) [45].…”

An integrated practical implementation of continuous aqueous two‐phase systems for the recovery of human IgG: From the microdevice to a multistage bench‐scale mixer‐settler device

“…This strategy using microscale continuous systems was explored here to perform a faster evaluation of different ATPS compositions for the most efficient recovery of the monoclonal antibody, exploiting the microfluidic advantages (reduced time, sample and reagents for the evaluation of a wide number of variables). Some continuous operation has been studied and documented in literature . Nevertheless, the growing market for the monoclonal antibodies and the constant need for more economically attractive large‐scale production processes make the studies of novel efficient continuous recovery processes more appealing .…”

1 Desterpenação Por Extração Líquido-Líquido De Mistura Modelo De Óleo De Bergamota Em Coluna PRDC

“…Com base nas características físico-químicas do sistema a ser trabalhado, diferentes tipos de equipamentos podem ser empregados na extração com solventes, a saber: misturadores-decantadores, extratores centrífugos e colunas de extração (Aguilar e Cortina, 2008). As colunas de extração apresentam a vantagem das fases se separarem facilmente e rapidamente pela força da gravidade, sem necessidade de uma centrifuga, sendo o processo simples e fácil de operar em modo contínuo (Porto et al, 2010) Dentre as diversas configurações de colunas extratoras, destaca-se a coluna de discos rotativos perfurados (Perforated rotating disc contactor, PRDC), pois apresenta vantagens em relação à simplicidade de construção, consumo de energia relativamente baixo, aumento da taxa e eficiência de transferência de massa que as perfurações proporcionam e alta eficiência proporcionada pelo movimento circular dos discos rotativos, os quais aumentam a turbulência do sistema (Porto et al, 2010).…”