Extraction of Functional Mitochondria Based on Membrane Stiffness

Rahman, Habibur; Xiao, Qinru; Zhao, Shirui; Wei, An-Chi; Ho, Yi‐Ping

doi:10.1007/978-1-0716-1266-8_26

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…59 MiR-101 has been found to be abnormally decreased in ovarian cancer, and its ectopic expression inhibits the production of the phosphatase and tensin homolog (PTEN) and PI3K/AKT, which in turn prevents the proliferation, invasion and migration of OC cells. 60 Numerous cancers, containing nonsmall cell lung, liver, breast, stomach, and esophageal cancers, as well as tumors of the nervous system, have shown to be influenced by miR-21, which has been linked to tumor formation and incidence. 101 In a study, it was shown that downregulating the expression of the miR-21 might mimic the curative effects of celastrol on OC cells, leading to a decrease in the expression levels of PI3K/Akt/NF-κB in OC cells.…”

Section: Ovarian Cancer (Oc)mentioning

confidence: 99%

“…The research shows that an intricate FoxP3‐miR‐150‐IGF1R/IRS1‐PI3K/AKT/mTOR feedback loop regulates the pathogenesis of OC, offering a new mechanism for miR‐150's role as a tumor inhibitor miRNA in OC 59 . MiR‐101 has been found to be abnormally decreased in ovarian cancer, and its ectopic expression inhibits the production of the phosphatase and tensin homolog (PTEN) and PI3K/AKT, which in turn prevents the proliferation, invasion and migration of OC cells 60 …”

Section: Pi3k/akt Regulation By Micrornas In Solid Tumorsmentioning

confidence: 99%

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Detailed role of microRNA‐mediated regulation of PI3K/AKT axis in human tumors

Al‐Hawary,

Ruzibakieva,

Gupta

et al. 2023

Cell Biochemistry & Function

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The regulation of signal transmission and biological processes, such as cell proliferation, apoptosis, metabolism, migration, and angiogenesis are greatly influenced by the PI3K/AKT signaling pathway. Highly conserved endogenous non‐protein‐coding RNAs known as microRNAs (miRNAs) have the ability to regulate gene expression by inhibiting mRNA translation or mRNA degradation. MiRNAs serve key role in PI3K/AKT pathway as upstream or downstream target, and aberrant activation of this pathway contributes to the development of cancers. A growing body of research shows that miRNAs can control the PI3K/AKT pathway to control the biological processes within cells. The expression of genes linked to cancers can be controlled by the miRNA/PI3K/AKT axis, which in turn controls the development of cancer. There is also a strong correlation between the expression of miRNAs linked to the PI3K/AKT pathway and numerous clinical traits. Moreover, PI3K/AKT pathway‐associated miRNAs are potential biomarkers for cancer diagnosis, therapy, and prognostic evaluation. The role and clinical applications of the PI3K/AKT pathway and miRNA/PI3K/AKT axis in the emergence of cancers are reviewed in this article.

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Section: Ovarian Cancer (Oc)mentioning

confidence: 99%

Section: Pi3k/akt Regulation By Micrornas In Solid Tumorsmentioning

confidence: 99%

Detailed role of microRNA‐mediated regulation of PI3K/AKT axis in human tumors

Al‐Hawary,

Ruzibakieva,

Gupta

et al. 2023

Cell Biochemistry & Function

View full text Add to dashboard Cite

show abstract

“…The purity of these crudely extracted mitochondria can meet some applications, including the analysis of the activity of known mitochondrial proteins, the detection of mitochondrial morphology and mitochondrial apoptosis; however, they often contain a certain amount of peroxisomes, endoplasmic reticulum and microsomes. Mitochondrial purity is low; thus, mitochondrial purification and reduction of membrane fouling are required when analyzing proteins present in multiple cells or determining the localization of a protein (17). Furthermore, although the mitochondrial extraction method is suitable for most tissues and cells, the extraction efficiency and quantity of mitochondria in different tissues and cells are significantly different.…”

Section: Extraction and Purification Of Mitochondriamentioning

confidence: 99%

Common methods in mitochondrial research (Review)

Yin

Shen

2022

Int J Mol Med

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Mitochondrial abnormalities are primarily seen in morphology, structure and function. They can cause damage to organs, including the heart, brain and muscle, by various mechanisms, such as oxidative stress, abnormal energy metabolism, or genetic mutations. Identifying and detecting pathophysiological alterations in mitochondria is the principal means of studying mitochondrial abnormalities. The present study reviewed methods in mitochondrial research and focused on three aspects: Mitochondrial extraction and purification, morphology and structure and function. In addition to classical methods, such as electron microscopy and mitochondrial membrane potential monitoring, newly developed methods, such as mitochondrial ultrastructural determination, mtdNA mutation assays, metabolomics and analyses of regulatory mechanisms, have also been utilized in recent years. These approaches enable the accurate detection of mitochondrial abnormalities and provide guidance for the diagnosis and treatment of related diseases. Contents1. Introduction 2. Extraction and purification of mitochondria 3. determination of mitochondrial morphology and structure 4. determination of mitochondrial function 5.

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Cellular organelles as drug carriers for disease treatment

Hu,

Liu,

et al. 2023

Journal of Controlled Release

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Extraction of Functional Mitochondria Based on Membrane Stiffness

Cited by 3 publications

References 14 publications

Detailed role of microRNA‐mediated regulation of PI3K/AKT axis in human tumors

Detailed role of microRNA‐mediated regulation of PI3K/AKT axis in human tumors

Common methods in mitochondrial research (Review)

Cellular organelles as drug carriers for disease treatment

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