Extraction optimization of neem bioactives from neem seed kernel by ultrasonic assisted extraction and profiling by UPLC-QTOF-ESI-MS

Saha, Supradip; Singh, Deeksha; Rangari, Shweta; Negi, Lalit Mohan; Banerjee, Tirthankar; Dash, Sukanta; Kundu, Aditi; Dutta, Anirban; Mandal, A.B.; Patanjali, Neeraj; Kumar, Rajesh; Kumar, Anil; Singh, Anupama

doi:10.1016/j.scp.2022.100747

Cited by 6 publications

(2 citation statements)

References 34 publications

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“…Similar work of oil extraction from neem seeds by Tesfaye and Tefera [9] and Djibril et al [19] showed an oil yield of 43.71% and 48.98 ± 0.34%, respectively. A little lower oil yield of 32.5% was obtained in neem seed kernels using 50% of ethanol as solvent by Saha et al [20]. Also, a maximum oil yield of 53.5% was obtained by Subramanian et al [21] at an optimum condition of extraction time (6 h) using a solvent mixture of 50 : 50 (n-hexane : ethanol).…”

Section: Efect Of the Drying Methods And Bed Tickness On Oil Yieldmentioning

confidence: 95%

Impact of Different Drying Techniques on Neem Seeds Drying Kinetics and Oil Quality

Kishore

Rajkumar

Sudha

et al. 2023

Journal of Food Quality

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Neem oil is a promising alternative for synthetic chemicals in food preservation and an active functional agent in food packaging. Drying studies were conducted on neem seeds using different drying methods, and the oil yield profile and nutritional content such as azadirachtin content, functional groups, and elemental composition were analysed. Tray drying at 60°C showed a faster drying rate with a minimum quality of the oil. The process parameters were statistically optimized by analysing the effect of drying methods and thickness (15 and 30 mm) on azadirachtin content and oil yield. Maximum oil yield and azadirachtin content of 42.1 and 0.053% were obtained in solar drying with 15 mm bed thickness. Fourier-transform infrared spectroscopy analysis showed that there is no change in the functional group when the neem seeds were dried, and the peak absorption wavenumber confirmed the presence of O-H stretching, C-H stretching, C-O stretching, C-H bending, O-H bending, C=O stretching, and N-H stretching. Sun- and solar-dried neem seeds showed maximum retention in elemental composition when compared to the tray-drying method.

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Section: Efect Of the Drying Methods And Bed Tickness On Oil Yieldmentioning

confidence: 95%

Impact of Different Drying Techniques on Neem Seeds Drying Kinetics and Oil Quality

Kishore

Rajkumar

Sudha

et al. 2023

Journal of Food Quality

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show abstract

“…Content Origin Reference Seeds 0.19%(w/w) India [70] 210.93 mg/g India [71] 0.08% (w/w) India [72] 3030.8 g/kg India [73] 0.56%-0.30% (w/w) India [73] 142-9,527 μg/g India [74] 0.08%-0.095% (w/w) India [75] 3,862.9-48,521 μg/g China [76] 6.5-8.2 mg/g China [77] 8.2 g/kg China [77] 0.0867% (w/w) Togo West Africa [78] 752 ppm Germany [79] 0.16%-0.27% (w/w) Brazil [80] 0.20%-0.51% (w/w) Brazil [81] 0.431% (w/w) Brazil [82] 0.476-3.09 mg/g Sudan [83] 2.24 g/kg Senegal [84] 5,419.08 pg/μg Mali [85] Callus 0.00005% (w/w) Togo West Africa [78] 0.2470 μg/g India [86] 214.53 mg/g DW India [87] Leaves 182.42 pg/μg Mali [85] 182.42 μg/g India [88] 969.9-5,419.08 μg/g China [76] 86.45 mg/g DW Iran [89] culture enable the in vivo biosynthesis of azadirachtin, the low yields fall short of meeting the actual demand.…”

Section: Samplementioning

confidence: 99%

A comprehensive review of azadirachtin: physicochemical properties, bioactivities, production, and biosynthesis

Su,

Liang,

Xue

et al. 2023

Acupuncture and Herbal Medicine

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Azadirachtin, a complex tetratriterpenoid limonin with potent insecticidal properties, is the most widely used biological pesticide worldwide. Its versatile pharmacological applications include the inhibition of tumor growth and anti-malarial, anti-bacterial, and anti-inflammatory properties. Azadirachtin plays a pivotal role in pest control and novel drug development. The primary source of azadirachtin is the neem tree (Azadirachta indica A. Juss), with an azadirachtin content ranging from 0.3% to 0.5%. Despite the market demand for botanical pesticides reaching approximately 100,000 tons per year, the annual neem production in China is only 1.14 tons. Although azadirachtin can be obtained through plant extraction or chemical synthesis, the quantity obtained does not meet the market demand in China. The sluggish pace of azadirachtin biosynthesis results from the limited availability of genetic information and the complexity of the synthetic pathway. Recent advancements in azadirachtin biosynthesis hold promise as an efficient collection method. In this study, we explored the physicochemical properties, biological activities, mechanisms of action, and acquisition methods of azadirachtin. We also delved into recent progress in azadirachtin biosynthesis and assessed potential future usage challenges. This study aims to establish a theoretical foundation for the scientific application and efficient synthesis of azadirachtin, offering valuable reference information to the industry.

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Greenness metrics for analytical methodologies linked to mass spectrometry

Jurisch,

Gomes,

Parreiras

et al. 2024

Green Analytical Chemistry

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Extraction optimization of neem bioactives from neem seed kernel by ultrasonic assisted extraction and profiling by UPLC-QTOF-ESI-MS

Cited by 6 publications

References 34 publications

Impact of Different Drying Techniques on Neem Seeds Drying Kinetics and Oil Quality

Impact of Different Drying Techniques on Neem Seeds Drying Kinetics and Oil Quality

A comprehensive review of azadirachtin: physicochemical properties, bioactivities, production, and biosynthesis

Greenness metrics for analytical methodologies linked to mass spectrometry

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