Extraction, Preliminary Characterization and Evaluation of in Vitro Antitumor and Antioxidant Activities of Polysaccharides from Mentha piperita

Abstract

This study describes the extraction, preliminary characterization and evaluation of the in vitro antitumor and antioxidant activities of polysaccharides extracted from Mentha piperita (MPP). The optimal parameters for the extraction of MPP were obtained by Box-Behnken experimental design and response surface methodology (RSM) at the ratio of water to raw material of 20, extraction time of 1.5 h and extraction temperature at 80 °C. Chemical composition analysis showed that MPP was mainly composed of glucuronic acid, galacturonic acid, glucose, galactose and arabinose, and the molecular weight of its two major fractions were estimated to be about 2.843 and 1.139 kDa, respectively. In vitro bioactivity experiments showed that MPP not only inhibited the growth of A549 cells but possessed potent inhibitory action against DNA topoisomerase I (topo I), and an appreciative antioxidant action as well. These results indicate that MPP may be useful for developing safe natural health products.

Metabolic profile and atherogenic indices of rats treated with Tamarindus indica and Mentha piperita juice.

“…These properties are due to the presence of bioactive compounds [23][24]. Sharafi et al [25] evaluated the protective activity of bioactive compounds in the essential oil of M.piperitaand showed similar results to our study.…”

Purification, characterization and immunomodulatory activity of a polysaccharide from Celosia cristata

“…In recent years, numerous polysaccharides isolated from natural resources have been proven to possess a wide range of biological properties and few adverse effects. These non-cytotoxic compounds, polysaccharides attracted attention as immunomodulators exhibiting variety beneficial pharmacological effects, such as anti-tumor, anti-inflammatory and anticomplementary activities (Kim, Cho, Karnjanapratum, Shin, & You, 2011;Zhao et al, 2014;Liu et al, 2014;Peng, Zhang, Zeng, & Kennedy, 2005;Chang, Lur, Lu, & Cheng, 2013;Benenica, Rodriguez, Matulewicz, & Coulombié, 1999). Hence, the study of chemical properties and immunomodulatory effects of polysaccharides is necessary.…”

Extraction, fractionation, and chemical characterisation of fucoidans from the brown seaweed Sargassum pallidum

“…The Agilent GPC software was used to calculate the MW value. The calibration curve of Log MW of the standard dextrans against their elution time (ET) was obtained (Log MW = -0.6946ET + 15.007, R 2 = 0.9985) (Liu et al 2014).…”