Extraction Procedures for Free Radical Scavenging Activity from Noni Fruit (Morinda citrifolia)

Gwak, Mi-kyung; Choi, Heesun; Manochai, Benya; Hong, Jeong-Hwa

doi:10.7783/kjmcs.2011.19.1.038

Cited by 8 publications

(3 citation statements)

References 37 publications

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“…Terdapat beberapa penelitian terkait pengaruh suhu terhadap zat berkahasiat alami pada jus mengkudu. Gwak et al (2011), melakukan penelitian perbandingan aktivitas antioksidan jus mengkudu yang diekstrak dengan metode aqueous extraction pada suhu 25 o C dan autoclave process extraction pada suhu 121 o C selama 2 jam ekstraksi. Hasil yang diperoleh menunjukkan terjadi penurunan kapasitas antioksidan pada jus mengkudu ekstrak buah mengkudu dari nilai DPHH 26,39 (ekstraksi suhu 25 o C) menjadi 15,34 (ekstraksi suhu 121 o C).…”

Section: Analisis Rf Skopoletin Dalam Produk Jus Mengkudu Hasil Evapounclassified

Peningkatan Konsentrasi Skopoletin dalam Jus Mengkudu (Morinda citrifolia L.) dengan Teknik Eliminasi dan Pemekatan Senyawa

Junaidi¹,

Hutajulu²,

Lestari³

et al. 2019

J.Wart.Industri.Has.Pertan.

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ABSTRAK: Jus mengkudu mengandung banyak komponen aktif yang bermanfaat untuk kesehatan, salah satunya adalah skopoletin. Namun demikian skopoletin tersebut berada dalam kandungan yang sangat kecil. Untuk meningkatkan kandungan skopoletin tersebut dapat dilakukan melalui proses pemekatan. Untuk itu perlu dilakukan penelitian peningkatan konsentrasi skopoletin dalam jus mengkudu (Morinda citrifolia L.) dengan teknik eliminasi dan pemekatan senyawa, untuk dapat memperoleh manfaat yang lebih baik dari jus mengkudu. Pemekatan dilakukan dengan dua cara yaitu freeze drying dan waterbath. Produk yang digunakan pada penelitian ini merupakan produk komersil yaitu: produk jus pekat (DS), produk premium/ekspor (PP) dan produk pasar lokal (EP). Hasil penelitian menunjukkan pemekatan dengan freeze drying diperoleh kadar skopoletin tertinggi dalam jus mengkudu DS sebesar 40,58 ppm. Sedangkan pemekatan menggunakan waterbath, kadar skopoletin tertinggi diperoleh pada jus mengkudu DS sebesar 0,308 ppm.

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Section: Analisis Rf Skopoletin Dalam Produk Jus Mengkudu Hasil Evapounclassified

Peningkatan Konsentrasi Skopoletin dalam Jus Mengkudu (Morinda citrifolia L.) dengan Teknik Eliminasi dan Pemekatan Senyawa

Junaidi¹,

Hutajulu²,

Lestari³

et al. 2019

J.Wart.Industri.Has.Pertan.

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“…Scopoletin has been isolated from many plants (Jain et al, 2002), and several biological activities had been reported, such as anti-in ammatory (Ding et al, 2008;Moon et al, 2007), antioxidant (Gwak et al, 2011;Shaw et al, 2003), anti-proliferative (Thani et al, 2010), anti-angiogenic (Pan et al, 2009), antithyroid, anti-hypertensive (Aldi et al, 2015), anti-hyperuricemic (Zeng et al, 2020), and anti-diabetic (Jang et al, 2020), among others.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory compounds produced in hairy roots culture of Sphaeralcea angustifolia

Reyes-Pérez

Herrera-Ruíz

Perea-Arango

et al. 2021

Plant Cell Tiss Organ Cult

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Sphaeralcea angustifolia (Cav) G. Don is used in traditional Mexican medicine to treat in ammations and gastric disease. Its anti-in ammatory and immunomodulatory activities in mice and rats acute and chronic models have been attributed mainly to scopoletin. Scopoletin reduced joint in ammation, the number of new vessels, production of endogenous angiogenic inducers, and reversed the histopathological alterations in rat adjuvant-induced arthritis. Tomentin and sphaeralcic acid from S. angustifolia cells in suspension proved anti-in ammatory and immunomodulatory activities in mice kaolin/λ-carrageenan-induced arthritis. Transformed roots of S. angustifolia have been proposed as active compounds producers. A high transformation frequency mediated by Agrobacterium rhizogenes ATCC15834/pTDT was obtained from nodal segments (59.5 ± 10.5%, 145 hairy root lines) and leaves (40.0 ± 25, 52 hairy root lines) of 2-month-old plantlets. Among seven lines selected according to their phenotypic characteristics and growth index, the SaTR N7.2 line presented the highest sphaeralcic acid production (17.6 ± 1.72 mg/g DW); this production was 440-fold superior to that reported in S. angustifolia wild plants, and in comparison to cells in the suspension of S. angustifolia in MS medium with nitrate restriction this was 263-fold higher when cultured in asks and 5-fold higher in a stirred-tank type bioreactor. The SaTR N7.2, SaTR N5.1, SaTR N7.1, and SaTR N15.1 lines excreted sphaeralcic acid into the culture medium at similar levels. Genetic transformation of hairy roots was con rmed by amplifying a 490 bp fragment of the rolC gene. S. angustifolia hairy root cultures producers of scopoletin and sphaeralcic acid can be stressed by nitrate reduction and/or copper increased to stimulate scopoletin and sphaeralcic acid production. Key MessageHairy roots lines of Sphaeralcea angustifolia, producers of scopoletin and sphaeralcic acid antiin ammatory compounds, were established by rst time through plant transformation mediated by Agrobacterium rhizogenes ATCC15834/pTDT.

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“…노니 에탄올 추출물은 합성 항산화제 중에 가장 널리 사용하고 있는 0.1% BHT와 비슷한 활성을 나타내었고 에탄올 추출물의 활성이 물 추출 물의 활성보다 높게 나타난 것으로 보고 되었다 [18]. 또한 Gwak 등의 선행 연구에서는 노니 과실 50% 에 탄올 추출물 5 mg/mL의 농도에서 DPPH 소거능이 71% 활성을 갖는다고 하였다 [28]. 따라서, 앞으로 노 니 추출물은 기능에 맞게 효과적인 방법으로 사용된 다면 항노화 기능성 화장품에의 응용이 가능하리라 사료된다.…”

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Study on the Bioactive Characteristics of Morinda citrifolia as a Cosmetic Raw Material

Kim¹,

Jang²

2016

Journal of the Society of Cosmetic Scientists of Korea

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This study attempted to investigate the possibility of the use of Morinda citrifolia (MC) as a cosmetic ingredient from its physiological activities such as antioxidant activity, cytotoxicity and anti-aging effect. MC is a tropical plant that has been used as traditional polynesian foods and medicines for over two thousand years. It has been reported that this shrub can improve antimicrobial, anti-cancer and anti-inflammatory effects and strengthen an immune system. The in vitro antioxidant activity of MC was performed to see the DPPH scavenging activity by measuring total polyphenol content and total flavonoid content. As a result, a lack of any cytotoxicity was confirmed in human dermal fibroblasts (HDF) cell. When MC extract at a concentration of over 50 ∼ 100 µg/mL was added, MMP-1 expression considerably diminished. In an in vivo test, in addition, cream containing MC extract was prepared and applied to a total of 22 women in their 30 ∼ 50s in ages in the morning and in the evening for four weeks. Changes in keratin, melanin index, pore, skin color and wrinkles under the naked eyes were then comparatively measured. Keratin levels slightly increased in the control group but decreased in the experimental group. In addition, wrinkles diminished in the experimental group. This study found that MC extract controls many MMP-1 related mechanisms with great potential for use as a natural ingredient of anti-aging cosmetics.

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Extraction Procedures for Free Radical Scavenging Activity from Noni Fruit (Morinda citrifolia)

Cited by 8 publications

References 37 publications

Peningkatan Konsentrasi Skopoletin dalam Jus Mengkudu (Morinda citrifolia L.) dengan Teknik Eliminasi dan Pemekatan Senyawa

Peningkatan Konsentrasi Skopoletin dalam Jus Mengkudu (Morinda citrifolia L.) dengan Teknik Eliminasi dan Pemekatan Senyawa

Anti-inflammatory compounds produced in hairy roots culture of Sphaeralcea angustifolia

Study on the Bioactive Characteristics of Morinda citrifolia as a Cosmetic Raw Material

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