Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis

Wang, Yonggang; Li, Yuanli; Ma, Xueqing; Ren, Haiwei; Fan, Wei; Leng, Feifan; Yang, Ming; Wang, Xiaoli

doi:10.1016/j.indcrop.2018.06.011

Cited by 91 publications

(32 citation statements)

References 52 publications

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“…This is also in contrast to what was reported the previous study mentioned above [31]. These results indicated that the physicochemical properties of these three GUPS fractions (extracted from G. uralensis from Xinjiang, China) are different from those polysaccharides purified by other groups from the same species but grown in different locations in previously reported studies (G. uralensis from Ningxia and from Gansu, China) [30,31]. The biotopes of G. uralensis from different places may cause the difference of polysaccharide accumulation, as well as the function of polysaccharides.…”

Section: Discussioncontrasting

confidence: 98%

“…These three fractions were analyzed qualitatively using HPGPC, GC-MS, SEM, FT-IR, and 1 H-NMR. The monosaccharide composition of GUPS-II is similar to that reported in previous studies, while GUPS-III has some differences, such as its mannose content [30,31]. GUPS-I and GUPS-II do not contain any protein, whereas GUPS-C and GUPS-III contain 4% and 21.5% protein, respectively.…”

Section: Discussionsupporting

confidence: 85%

“…The sulfate contents of GUPS-I (6.5%) and GUPS-II (8.7%) are similar to those reported in previous studies, while the sulfate content of GUPS-III (20.47%) is much higher than that in previous reports (5.43% and 3.42%) [29,30]. GUPS-II and GUPS-III are flaky without a porous structure, which is in contrast to data from a previous study [31]. GUPS-II and GUPS-III both show a smooth surface, suggesting that they may form an amorphous structure.…”

Section: Discussionsupporting

confidence: 83%

“…The results showed that GUPS-I was composed of Rha, Xyl, and Glc at molar ratios of 2.55:1.9:30.03, GUPS-II consisted of Rha, Ara, Man, Glc, and Gal at molar ratios of 1:13.87:1.59:16.76:15.72, and GUPS-III was composed of Ara, Man, Glc, and Gal at molar ratios of 1:5.33:1.91:5.97 ( Figure 2). The monosaccharide composition of GUPS-II was similar to that determined in previous studies, but GUPS-III had some differences, such as the mannose content [30,31].…”

Section: Monosaccharide Composition Of Gupssupporting

confidence: 82%

“…GUPS-I might be a neutral polysaccharide because it coeluted with distilled water, whereas both GUPS-II and GUPS-III, which eluted with NaCl solution, might be acidic polysaccharides [29]. The results are similar to previous studies which reported that three major fractions can be obtained from crude polysaccharides of G. uralensis [30,31], but different from another study which reported that five fractions can be observed [46].…”

Section: Discussionsupporting

confidence: 67%

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Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

et al. 2020

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Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities. Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively. The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different. The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages. Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I. An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs. These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.

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Section: Discussioncontrasting

confidence: 98%

Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 83%

Section: Monosaccharide Composition Of Gupssupporting

confidence: 82%

Section: Discussionsupporting

confidence: 67%

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Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

et al. 2020

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Feruloyl oligosaccharides, isolated from bacterial fermented wheat bran, exhibit antioxidant effects in IPEC‐J2 cells and zebrafish model

Chen

Zhang

Wang

et al. 2022

Food Science & Nutrition

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Feruloyl oligosaccharides (FOs) were produced by solid‐state fermentation of wheat bran using Bacillus subtilis, Bacillus licheniformis, and Saccharomyces cerevisiae, and its antioxidant activity was investigated using IPEC‐J2 cells and zebrafish embryo model. Preliminary structure analysis revealed that FOs has an average molecular weight of 11.81 kDa and consists of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose. The obtained FOs possess superior reducing power and DPPH and hydroxyl free radical scavenging activities. In IPEC‐J2 cells, antioxidant enzymes activities and GSH level were significantly increased, while MDA level was reduced by FOs. Further studies showed that FOs achieved the aforementioned effects by activating Nrf2 signaling pathway. In zebrafish embryo, FOs effectively suppressed ROS production, lipid peroxidation, and cell death by increasing SOD and GSH‐Px activities. Our findings suggested that FOs from solid‐state fermented wheat bran with mixed bacteria can be used as an antioxidant food additive or drugs.

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A novel polysaccharide from Macadamia peel: Extraction, purification, structural characterization and antioxidant activity

Guo,

Xu,

et al. 2024

J Sci Food Agric

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BACKGROUNDMacadamia peels are the main by‐product of postharvest treatment of the whole fresh fruit weight, they contain various bioactive substances, such as polysaccharides, phenols, flavonoids, phenolic acids, saponins, and other nutritional and functional components, that are known to have anti‐tumor and anti‐oxidation functions.RESULTSTwo purified polysaccharide fractions were obtained (MPP‐1 and MPP‐2) by extracting with ultrasonic‐microwave‐aided water extraction and purifying by with DEAE‐52 and Sephadex G‐50 columns, and then characterized using Fourier‐transform infrared spectroscopy, scanning electron microscopy, atomic force microscopy and NMR, and the antioxidant activity was also investigated. The results indicated that MPP‐1 and MPP‐2 were mainly glucose, the molecular weight was 8.16 kDa and 7.73 kDa, respectively. Methylation with gas chromatography‐mass spectrometry and NMR analyses confirmed that two fractions comprised of →4) ‐α‐d‐ Glcp ‐(1→, →6) ‐β‐d‐ Glcp ‐(1→ and →3,4) ‐β‐d‐ Glcp ‐(1→) as the main chain. In addition, MPP‐1 and MPP‐2 polysaccharides showed significant antioxidant activity with respect to 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), hydroxyl group and reducing power, and MPP‐2 demonstrated excellent antioxidant activities compared to MPP‐1 with IC50 values of 35.12, 18.82 and 40.12 μg mL−1, respectively.CONCLUSIONThe novel polysaccharide, MPP‐1 and MPP‐2, mainly containing glucose, showed significant antioxidant activity with respect to DPPH, hydroxyl group and reducing power. This study has enhanced waste utilization and reduced environmental pollution, providing some inspiration for the reuse of the waste generated during agricultural production. It may bring good prospects for their use as antioxidants in functional foods. © 2024 Society of Chemical Industry.

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Extraction, purification, and bioactivities analyses of polysaccharides from Glycyrrhiza uralensis

Cited by 91 publications

References 52 publications

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

Feruloyl oligosaccharides, isolated from bacterial fermented wheat bran, exhibit antioxidant effects in IPEC‐J2 cells and zebrafish model

A novel polysaccharide from Macadamia peel: Extraction, purification, structural characterization and antioxidant activity

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