Extraction, Purification, and Biological Activities of Polysaccharides from Branches and Leaves of Taxus cuspidata S. et Z.

Abstract

Taxus cuspidata S. et Z. is an excellent natural source of bioactive polysaccharides and has various biological activities. The objective of this study was to evaluate the effect of antidiabetic and antitumor activities of polysaccharides from Taxus cuspidata branches and leaves (TCBL) and to determine the optimum extraction technology of TCBL using a low-temperature and high-efficiency enzyme and ultrasound-assisted coupled extraction (EUCE) method. Optimal technology parameters were determined as follows: an extraction temperature of 51 °C, an extraction time of 33 min, a ratio of material to liquid of 1:19 (g:mL), and an enzyme concentration of 0.10 mg·mL−1. Under the optimized conditions, the polysaccharide yield from TCBL obtained by EUCE was 4.78% ± 0.18%. The four purified polysaccharides (Pe1, Pe2, Pe3, Pe4) from TCBL are mainly composed of arabinose, galactose, glucose, a small amount of xylose, and mannose. This composition was assessed by HPIC analysis. The antidiabetic activity and antitumor activity of polysaccharides from TCBL were assayed in vitro. Among the four purified polysaccharides from TCBL, purified Pe4 had the highest inhibitory capacity against α-glucosidase, and its IC50 value was 123.0 µg·mL−1. Pe1 had the highest antitumor capacity against MCF7 cells and HepG2 cells, with IC50 values of 169.0 and 132.0 µg·mL−1. Pe4 had the highest antitumor effect on human cervical cancer cells (Hela), and its IC50 value was 89.9 µg·mL−1. Pe4 polysaccharide demonstrated a good α-glucosidase inhibitory activity and antitumor capacity against Hela cells. Therefore, Pe4 polysaccharide from TCBL is a beneficial source of potential inhibitors of type II diabetes and human cervical cancer activity.

Unveiling the culturable and non‐culturable actinobacterial diversity in two macroalgae species from the northern Portuguese coast

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Separation, Purification, Structural Characterization, and Anticancer Activity of a Novel Exopolysaccharide from Mucor sp.

“…Analysis of monosaccharide composition was performed on a high-performance ion exchange chromatography (HPIC) system, equipped with a Dionex™ Carbopac™ PA20 column (ICS5000, 3 × 150 mm, ThermoFisher, MA, USA) [ 44 ]. MSEPS was hydrolyzed by 10 mL of 3 M trifluoroacetic acid (TFA) at 120 °C for 3 h. The MSEPS hydrolysates were dried with nitrogen to remove TFA.…”

Research Progress on Taxus Extraction and Formulation Preparation Technologies

“…was optimized. The optimized conditions are: extraction temperature of 51 • C, extraction time of 33 min, solid-liquid ratio of 1:19 (g:mL), enzyme concentration of 0.10 mg/mL, with a polysaccharide yield of 4.78% ± 0.18% [64]. The extracted Taxus polysaccharides have significant biological activities, including inhibiting α-glucosidase and anti-tumor effects on various cancer cells, indicating the effectiveness of this method and the potential for extracting bioactive compounds from Taxus.…”