Extraction, purification, characterization and antioxidant activities of polysaccharides from Ramaria botrytis (Pers.) Ricken

Li, Hua

doi:10.1186/s13065-017-0252-x

Cited by 19 publications

(17 citation statements)

References 22 publications

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“…This glucan showed immunostimulating activity on RAW 264.7, a murine macrophage cell line, by nitric oxide production [198]. Moreover, polysaccharides from R. botrytis showed potent antioxidant activities [199]. Fresh fruiting bodies of the mushroom collected in Japan have been reported to contain (2S,2 R,3R,4E,8E)-N-2 -hydroxyoctadecanoyl-2-amino-9-methyl-4,8-heptadecadiene-1,3-diol, 5α,6α-epoxy-3β-hydroxy-(22E)-ergosta-8(14),22-dien-7-one, ergosterol peroxide, cerevisterol and 9α-hydroxycerevisterol [200].…”

Section: Ramaria Botrytis (Pers) Bourdotmentioning

confidence: 98%

“…Polysaccharide [199], Extract [202][203][204] -A novel ubiquitin-like protein [196] Glucan (immunostimulating activity) [198] Rugiboletus extremiorientalis (Lj.N. Vassiljeva) G. Wu & Zhu L. Yang ---Leccinine A (84) (protective activity against endoplasmic reticulum stress-dependent cell death [205] and plant growth regulatory activity [206]).…”

Section: Thelephora Ganbajun M Zangmentioning

confidence: 99%

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Bioactive Phytochemical Constituents of Wild Edible Mushrooms from Southeast Asia

et al. 2020

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Mushrooms have a long history of uses for their medicinal and nutritional properties. They have been consumed by people for thousands of years. Edible mushrooms are collected in the wild or cultivated worldwide. Recently, mushroom extracts and their secondary metabolites have acquired considerable attention due to their biological effects, which include antioxidant, antimicrobial, anti-cancer, anti-inflammatory, anti-obesity, and immunomodulatory activities. Thus, in addition to phytochemists, nutritionists and consumers are now deeply interested in the phytochemical constituents of mushrooms, which provide beneficial effects to humans in terms of health promotion and reduction of disease-related risks. In recent years, scientific reports on the nutritional, phytochemical and pharmacological properties of mushroom have been overwhelming. However, the bioactive compounds and biological properties of wild edible mushrooms growing in Southeast Asian countries have been rarely described. In this review, the bioactive compounds isolated from 25 selected wild edible mushrooms growing in Southeast Asia have been reviewed, together with their biological activities. Phytoconstituents with antioxidant and antimicrobial activities have been highlighted. Several evidences indicate that mushrooms are good sources for natural antioxidants and antimicrobial agents

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Section: Ramaria Botrytis (Pers) Bourdotmentioning

confidence: 98%

Section: Thelephora Ganbajun M Zangmentioning

confidence: 99%

Bioactive Phytochemical Constituents of Wild Edible Mushrooms from Southeast Asia

et al. 2020

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“…The reducing power is an important index to evaluate the antioxidant activity of a compound, which could directly reflect the production condition of electron donor. [34] The reducing power of Inonotus cuticularis polysaccharides determined at 700 nm was presented in Figure 4d. The reducing power of polysaccharides sample appeared linear growth as the sample concentration increased from 0.05 to 0.30 mg/mL.…”

Section: Reducing Power Assaymentioning

confidence: 99%

“…The * OH scavenging activity of Inonotus cuticularis polysaccharides was determined according to a report [34] with slight modification. 1.0 mL sample solutions (0.05, 0.10, 0.15, 0.20, 0.25 and 0.30 mg/mL) were mixed with ferrous sulfate solution (0.2 mL, 3 m mol/L), salicylic acid-ethanol solution (0.2 mL, 6 m mol/ L) and hydrogen peroxide solution (0.2 mL, 4 m mol/L), respectively.…”

Section: Oh Scavenging Activitymentioning

confidence: 99%

Orthogonal Test Design for Optimization of the Extraction of Polysaccharides from Inonotus cuticularis and Their Antioxidant Activities

Guo

et al. 2020

Chemistry & Biodiversity

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Medical fungi polysaccharides belong to a very important species of biological macromolecules, which are the basic substances that effectively maintain and ensure the normal operation of biological life activities. However, research on extraction and biological activity of Inonotus cuticularis polysaccharides has never been reported. In this study, the optimum yield of Inonotus cuticularis polysaccharides was determined by the orthogonal experimental design. The highest yield of 3.10±0.06 % was obtained with extraction temperature of 80 °C, extraction time of 150 min, and water to raw material ratio of 30 mL/g and repeated twice. After deproteinization for 5 times, the protein removal rate reached 70.10±1.75 %, and the content of polysaccharides and protein were 46.64 and 0.42 %. Infrared spectrometer indicated that Inonotus cuticularis polysaccharides are typical β‐pyranose with characteristic peaks of polysaccharides. Subsequently, the activities of scavenging free radicals for the deproteinated polysaccharides were studied. When the concentration of Inonotus cuticularis polysaccharides was 0.3 mg/mL, the scavenging activities of the sample on DPPH., .OH, ABTS.+ and O2.− reached 83.67±0.27, 65.21±4.82, 43.45±1.36 and 80.28±2.30 %, respectively, and the reducing power reached 0.46±0.01. The IC50 values scavenging DPPH., .OH, ABTS.+ and O2.− were 0.139±0.13, 0.162±0.14, 0.317±0.30 and 0.121±0.10 mg/mL, respectively. Results showed that Inonotus cuticularis polysaccharides present potential stronger antioxidant activities, especially .OH scavenging activity and reducing power. Experimental results could provide research basis of Inonotus cuticularis polysaccharides for further exploitation and utilization.

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“…At present, various extraction techniques are used to extract SG. New extraction techniques such as ultrasound‐assisted extraction (UAE) and microwave‐assisted extraction (MAE) have also shown significant development (Fan et al, ; Jaitak, Bandna, & Kaul, ; Jiang, Wang, Li, & Zhang, ; Li, ; Liu et al, ; Sun, Gu, & Yang, ). But conventional solvent extraction (SE) is still the most commonly applied method (Golubev & Beglov, ; Sophie & Martin, ; Xing & Xing, ).…”

Section: Introductionmentioning

confidence: 99%

Extraction of steviol glycosides from Stevia rebaudiana (Bertoni) leaves by high‐speed shear homogenization extraction

Wang

Guo

et al. 2019

J Food Process Preserv

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An efficient high‐speed shear homogenization extraction (HSHE) technique was employed for extracting stevioside (STV) and rebaudioside A (RA) from Stevia rebaudiana (Bertoni) leaves. The HSHE extraction procedure was optimized and compared with other conventional extraction techniques. HSHE showed a better extraction efficiency than solvent extraction (SE), ultrasound‐assisted extraction (UAE), and microwave‐assisted extraction (MAE) methods. Based on single‐factor experiments, the extraction conditions of HSHE method were optimized by response surface method (RSM) modeling. The optimal conditions were 59% ethanol aqueous solution as extraction agent, 8 min extraction time, and 68°C extraction temperature. The yields of STV, RA, and rebaudioside C (RC) were 5.37 ± 0.10%, 8.68 ± 0.13%, and 0.99 ± 0.03%, respectively. In addition, the contents of other components in crude extract, such as pigments, proteins, and pectin, extracted by HSHE, were compared with SE, UAE, and MAE methods. The result showed that the HSHE method has the advantages of low extraction temperature, high efficiency, and low time and energy consumption. The study demonstrated that HSHE has a great potential for the extraction of steviol glycosides from S. rebaudiana (Bertoni) leaves. Practical applications Steviol glycosides (SGs) are a new natural sweetener with high safety, good stability, and nonfermentation, which is herbal plant of the composite extracted from S. rebaudiana (Bertoni). It has the characteristics of high sweetness and low thermal energy. Its safety has been approved by the European Food Safety Administration (EFSA) and Joint Committee of Experts on Food Additives (JECFA). Generally speaking, SG is extracted from S. rebaudiana (Bertoni) by SE, UAE, and MAE methods. However, these methods have the disadvantages of low efficiency, low yield, and high solvent consumption. At present, there is no literature reported on the extraction of SG from S. rebaudiana (Bertoni) by HSHE method. Therefore, this study is to optimize the extraction process of HSHE and compare the contents of impurities in the crude extracts of different extraction methods, in order to provide a new method for extraction of SG from S. rebaudiana (Bertoni).

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Extraction, purification, characterization and antioxidant activities of polysaccharides from Ramaria botrytis (Pers.) Ricken

Cited by 19 publications

References 22 publications

Bioactive Phytochemical Constituents of Wild Edible Mushrooms from Southeast Asia

Bioactive Phytochemical Constituents of Wild Edible Mushrooms from Southeast Asia

Orthogonal Test Design for Optimization of the Extraction of Polysaccharides from Inonotus cuticularis and Their Antioxidant Activities

Extraction of steviol glycosides from Stevia rebaudiana (Bertoni) leaves by high‐speed shear homogenization extraction

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