Extraction, Structure and Immunoregulatory Activity of Low Molecular Weight Polysaccharide from Dendrobium officinale

Abstract

The ethanol precipitation method has been widely-used for Dendrobium officinale polysaccharides preparation. However, the alcohol-soluble fractions have always been ignored, which causes significant wastes of resources and energies. In this study, the extraction, physicochemical properties, and immune regulation activity of an edible D. officinale polysaccharide (DOPs) isolated from the supernatant after 75% ethanol precipitation were systematically investigated. The structural characteristics determination results showed that DOPs was mainly composed of glucose and mannose at a molar ratio of 1.00:5.78 with an average molecular weight of 4.56 × 103 Da, which was made up of α-(1,3)-Glcp as the main skeleton, and the α-(1,4)-Glcp and β-(1,4)-Manp as the branches. Subsequently, the cyclophosphamide (CTX)-induced immunosuppressive mice model was established, and the results demonstrated that DOPs could dose-dependently protect the immune organs against CTX damage, improve the immune cells activities, and promote the immune-related cytokines (IL-2, IFN-γ and TNF-α) secretions. Furthermore, DOPs treatment also effectively enhanced the antioxidant enzymes levels (SOD, GSH-Px) in sera and livers, therefore weakening the oxidative damage of CTX-treated mice. Considering these above data, DOPs presented great potential to be explored as a natural antioxidant and supplement for functional foods.

Modification of the Textural Properties of Chitosan to Obtain Biochars for CO2-Capture Processes

“…In addition, the second contribution located about 286.0 eV is attributed to the C-N or C-O groups, thus confirming that O and N-species are present in the carbonaceous structure in the form of amorphous graphite. At higher binding energies, approximately 287.0 eV, other small contributions assigned to C=O can be observed, while the band located at higher binding energy in this region (289.0 eV) is attributed to the presence of the O-C=O groups [ 40 , 41 ].…”

Promising Role of Polylactic Acid as an Ingenious Biomaterial in Scaffolds, Drug Delivery, Tissue Engineering, and Medical Implants: Research Developments, and Prospective Applications

“…[ 129 , 130 , 131 ]. To overcome these problems, PLA is coupled with various other biocompatible polymers for the fabrication of nano formulations used in medical therapeutic deliveries as approved by the FDA [ 132 , 133 , 134 ]. For instance, a formulation based on PLGA-b-PEG-b-PLGA of size 77–84 nano meter (nm) has the advantage of sustaining oral formulation and therefore extensively used in oncology [ 135 ].…”

Emerging Biopharmaceuticals from Pimpinella Genus

“…Pseudoisoeugenol, as a representative chemical marker of Pimpinella , possessed a peculiar skeleton of 1-hydroxy-2-propyl-4-methoxybenzene, which has been found exclusively in the genus Pimpinella so far [ 28 ]. Since the first pseudoisoeugenol was discovered from the P. anisum by G. T. Carter et al [ 29 ] in 1977, scientists have successively obtained 12 pseudoisoeugenol ( 1 – 12 ) from the Chinese herb P. thellungiana , and llungianin A ( 1 ) and llungianin B ( 2 ), which presented significant antihypertensive activity [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ].…”