Extractive fermentation for enhanced production of alkaline phosphatase from Bacillus licheniformis MTCC 1483 using aqueous two-phase systems

Pandey, Sarita; Banik, Rathindra Mohan

doi:10.1016/j.biortech.2010.12.066

Cited by 47 publications

(19 citation statements)

References 33 publications

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“…On the other hand, increasing the temperature the proteins denature and lose its activity. Similar temperature effect were found for various biomolecules such as globular proteins from tannery wastewater (Saravanan et al 2006 tannase from fermentation broth (Naidu et al 2008), alkaline phosphatase from Bacillus licheniformis (Pandey and Banik 2010), bromelain from pineapple (Ketnawa et al 2010), BSA (Perumalsamy and Batcha 2011) and β-glucosidase from Trichoderma reesei (Gautam and Simon 2006).…”

Section: Effect Of Temperature On Protein Partitioningsupporting

confidence: 58%

Aqueous two phase partitioning of fish proteins: partitioning studies and ATPS evaluation

Nagaraja

Iyyaswami

2014

J Food Sci Technol

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A suitable Aqueous Two Phase System (ATPS) has been identified for the partitioning of crude fish proteins from fish processing industrial effluent. A detailed study has been performed to analyze the influence of various parameters on protein partitioning such as molecular weight of PEG, effect of different salts (MgSO4 , K 2 HPO 4, Na 3 C 6 H 5 O 7, Na 2 SO 4, (NH 4 ) 2 PO 4, K 3 C 6 H 5 O 7 ) and their concentrations, pH, temperature, Tie Line Length (TLL), effluent loading and volume ratio. PEG 2000 -sodium sulphate ATPS was found to be a most favourable system among the selected ATPS for higher partition coefficient of proteins. The binodal curve and equilibrium characteristics of PEG 2000 -sodium sulphate were established and fitted to empirical equations. The equilibrium compositions (tie line) were correlated using Othmer-Tobias and Bancroft equations.

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Section: Effect Of Temperature On Protein Partitioningsupporting

confidence: 58%

Aqueous two phase partitioning of fish proteins: partitioning studies and ATPS evaluation

Nagaraja

Iyyaswami

2014

J Food Sci Technol

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“…The partition coefficients obtained varied between 0.88 and 1.28, which in general terms indicates an even enzyme fractionation, as occurred in the present study. Pandey and Banik assayed the extractive fermentation of an alkaline phosphatase using PEG (2,000, 4,000, and 6,000 g/mol)‐DEX (T500 and 40,000 g/mol) ATPS . The enzyme partitioned preferably towards the top phase, especially in systems prepared with DEX T500.…”

Section: Resultsmentioning

confidence: 99%

Potential application of aqueous two‐phase systems and three‐phase partitioning for the recovery of superoxide dismutase from a clarified homogenate of Kluyveromyces marxianus

Simental-Martínez

Rito‐Palomares

Benavides

2014

Biotechnology Progress

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Superoxide dismutase (SOD; EC 1.15.1.1) is an antioxidant enzyme that represents the primary cellular defense against superoxide radicals and has interesting applications in the medical and cosmetic industries. In the present work, the partition behavior of SOD in aqueous two-phase systems (ATPS) (using a standard solution and a complex extract from Kluyveromyces marxianus as sample) was characterized on different types of ATPS (polymer-polymer, polymer-salt, alcohol-salt, and ionic liquid (IL)-salt). The systems composed of PEG 3350-potassium phosphate, 45% TLL, 0.5 M NaCl (315 U/mg, 87% recovery, and 15.1-fold purification) and t-butanol-20% ammonium sulfate (205.8 U/mg, 80% recovery and 9.8-fold purification), coupled with a subsequent 100 kDa ultrafiltration stage, allowed the design of a prototype process for the recovery and partial purification of the product of interest. The findings reported herein demonstrate the potential of PEG-salt ATPS for the potential recovery of SOD.

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“…Several ALPs have been investigated from the strains of bacteria and fungi such as the hyperthermophilic archaeon Pyrococcus abyssi (Zappa et al, 2001); the thermophile Thermotoga neapolitana and Thermus thermophilus (Pantazaki et al, 1998), the hyperthermophilic archaea, Aeropyrum pernix (Helianti et al, 2007), Bacillus sphaericus (Dhaked et al, 2005), Bacillus sp. (Mahesh et al, 2010) and E.coli EFRL 13 (Qureshi et al, 2010), Bacillus licheniformis MTCC1483 (Pandey & Banik, 2011), Rhizopus icrospores var. rhizopodiformis (Junior et al, 2008).…”

Section: Isolation and Screening Of Alp-producing Halophillic Bacteriamentioning

confidence: 99%

Characterization of Alkaline Phosphatase Producing Bacteria Isolated from Thai Fermented Fish Products

Sitdhipol¹,

Niwasabutra²,

Wannissorn³

et al. 2012

IJB

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Seventeen isolates of alkaline phosphatase (ALP) producing bacteria were screened and systematically studied. They were divided into 10 groups on the basis of their phenotypic characteristics and 16S rRNA gene sequence analyses. The Gram-positive coccal isolates in Group I (3 isolates), Group II (3 isolates) and Group III (1 isolate) showed 99.2%, 99.5-99.7% and 99.8% sequences similarity to Staphylococcus saprophyticus, S. napalensis and S. sciuri, respectively. Each Gram-positive rod-shaped isolates in Group IV and V belonged to the genus Bacillus and was closely related to B. vietnamensis and B. safensis with 99.2% and 99.4% sequences similarity, respectively. The Gram-positive, moderately halophilic rod-shaped bacteria in Group VI (3 isolates), Group VII (1 isolate), Group VIII (1 isolate) and Group IX (2 isolates) were closely related to Virgibacillus halodenitrificans (98.5-99.1%), Oceanobacillus iheyensis (99.3%), Halobacillus mangrove (97.9%) and H. dabanensis (98.5-98.6%), respectively. One Gram-negative isolate of moderately halophilic bacterium (Group X) was closely related to Idiomarina zobelli (98.0%). They possessed phosphatase activities ranged from 10.08-70.96 U ml-1 . The isolate NSW 13-2 (Group VI) showed the highest ALP.

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Extractive fermentation for enhanced production of alkaline phosphatase from Bacillus licheniformis MTCC 1483 using aqueous two-phase systems

Cited by 47 publications

References 33 publications

Aqueous two phase partitioning of fish proteins: partitioning studies and ATPS evaluation

Aqueous two phase partitioning of fish proteins: partitioning studies and ATPS evaluation

Potential application of aqueous two‐phase systems and three‐phase partitioning for the recovery of superoxide dismutase from a clarified homogenate of Kluyveromyces marxianus

Characterization of Alkaline Phosphatase Producing Bacteria Isolated from Thai Fermented Fish Products

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