Extracts obtained from predegenerated nerves improve functional recovery after sciatic nerve transection

Marcol, Wiesław; Kotulska, Katarzyna; Larysz‐Brysz, Magdalena; Matuszek, Iwona; Olakowska, Edyta; Lewin–Kowalik, Joanna

doi:10.1002/micr.20155

Cited by 21 publications

(9 citation statements)

References 42 publications

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“…NGCs (Galla et al, 2004;Lee et al, 2003;Marcol et al, 2005), and can promote cell adhesion and ingrowth as a result of its numerous binding sites for integrins (Thiagarajan et al, 1996) and SCs.…”

Section: Discussionmentioning

confidence: 99%

Fibrin matrices with affinity‐based delivery systems and neurotrophic factors promote functional nerve regeneration

Wood

MacEwan

French

et al. 2010

Biotech & Bioengineering

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Glial-derived neurotrophic factor (GDNF) and nerve growth factor (NGF) have both been shown to enhance peripheral nerve regeneration following injury and target different neuronal populations. The delivery of either growth factor at the site of injury may, therefore, result in quantitative differences in motor nerve regeneration and functional recovery. In this study we evaluated the effect of affinity-based delivery of GDNF or NGF from fibrin-filled nerve guidance conduits (NGCs) on motor nerve regeneration and functional recovery in a 13 mm rat sciatic nerve defect. Seven experimental groups were evaluated consisting of GDNF or NGF and the affinity-based delivery system (DS) within NGCs, control groups excluding the DS and/or growth factor, and nerve isografts. Groups with growth factor in the conduit demonstrated equivalent or superior performance in behavioral tests and relative muscle mass measurements compared to isografts at 12 weeks. Additionally, groups with GDNF demonstrated greater specific twitch and tetanic force production in extensor digitorum longus (EDL) muscle than the isograft control, while groups with NGF produced demonstrated similar force production compared to the isograft control. Assessment of motor axon regeneration by retrograde labeling further revealed that the number of ventral horn neurons regenerating across NGCs containing GDNF and NGF DS was similar to the isograft group and these counts were greater than the groups without growth factor. Overall, the GDNF DS group demonstrated superior functional recovery and equivalent motor nerve regeneration compared to the isograft control, suggesting it has potential as a treatment for motor nerve injury.

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Section: Discussionmentioning

confidence: 99%

Fibrin matrices with affinity‐based delivery systems and neurotrophic factors promote functional nerve regeneration

Wood

MacEwan

French

et al. 2010

Biotech & Bioengineering

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“…Fibrin has also been used as a biomaterial scaffold to support neural regeneration within an NGC [11–13] and may offer an advantage over other materials because it naturally forms within an empty silicone conduit connecting the damaged ends of rat sciatic nerve [14]. Furthermore, fibrin contains sites for cell binding via integrin receptors [15], including cell binding sites for Schwann cells [16], which may facilitate cellular migration.…”

Section: Introductionmentioning

confidence: 99%

Affinity-based release of glial-derived neurotrophic factor from fibrin matrices enhances sciatic nerve regeneration

et al. 2009

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Glial-derived neurotrophic factor (GDNF) promotes both sensory and motor neuron survival. The delivery of GDNF to the peripheral nervous system has been shown to enhance regeneration following injury. In this study we evaluated the effect of affinity-based delivery of GDNF from a fibrin matrix in a nerve guidance conduit on nerve regeneration in a 13 mm rat sciatic nerve defect. Seven experimental groups were evaluated which received GDNF or nerve growth factor (NGF) with the delivery system within the conduit, control groups excluding one or more components of the delivery system, and nerve isografts. Nerves were harvested 6 weeks after treatment for analysis by histomorphometry and electron microscopy. The use of the delivery system (DS) with either GDNF or NGF resulted in a higher frequency of nerve regeneration vs. control groups, as evidenced by a neural structure spanning the 13 mm gap. The GDNF DS and NGF DS groups were also similar to the nerve isograft group in measures of nerve fiber density, percent neural tissue and myelinated area measurements, but not in terms of total fiber counts. In addition, both groups contained a significantly greater percentage of larger diameter fibers, with GDNF DS having the largest in comparison to all groups, suggesting more mature neural content. The delivery of GDNF via the affinity-based delivery system can enhance peripheral nerve regeneration through a silicone conduit across a critical nerve gap and offers insight into potential future alternatives to the treatment of peripheral nerve injuries.

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“…[8][9][10][11][12][13][14] Nerve growth factor (NGF) was first recognized for its stimulatory effect on nerve fiber growth. Local sources of NGF can dictate the direction of this neuritic growth.…”

mentioning

confidence: 99%

Improvement of peripheral nerve regeneration in acellular nerve grafts with local release of nerve growth factor

Jiang

Guo

et al. 2009

Microsurgery

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Previous studies have demonstrated the potential of growth factors in peripheral nerve regeneration. A method was developed for sustained delivery of nerve growth factor (NGF) for nerve repair with acellular nerve grafts to augment peripheral nerve regeneration. NGF-containing polymeric microspheres were fixed with fibrin glue around chemically extracted acellular nerve grafts for prolonged, site-specific delivery of NGF. A total of 52 Wistar rats were randomly divided into four groups for treatment: autografting, NGF-treated acellular grafting, acellular grafting alone, and acellular grafting with fibrin glue. The model of a 10-mm sciatic nerve with a 10-mm gap was used to assess nerve regeneration. At the 2nd week after nerve repair, the length of axonal regeneration was longer with NGF-treated acellular grafting than acellular grafting alone and acellular grafting with fibrin glue, but shorter than autografting (P < 0.05). Sixteen weeks after nerve repair, nerve regeneration was assessed functionally and histomorphometrically. The percentage tension of the triceps surae muscles in the autograft group was 85.33 +/- 5.59%, significantly higher than that of NGF-treated group, acellular graft group and fibrin-glue group, at 69.79 +/- 5.31%, 64.46 +/- 8.48%, and 63.35 +/- 6.40%, respectively (P < 0.05). The ratio of conserved muscle-mass was greater in the NGF-treated group (53.73 +/- 4.56%) than in the acellular graft (46.37 +/- 5.68%) and fibrin glue groups (45.78 +/- 7.14%) but lower than in the autograft group (62.54 +/- 8.25%) (P < 0.05). Image analysis on histological observation revealed axonal diameter, axon number, and myelin thickness better with NGF-treated acellular grafting than with acellular grafting alone and acellular grafting with fibrin glue (P < 0.05). There were no significant differences between NGF-treated acellular grafting and autografting. This method of sustained site-specific delivery of NGF can enhance peripheral nerve regeneration across short nerve gaps repaired with acellular nerve grafts.

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Extracts obtained from predegenerated nerves improve functional recovery after sciatic nerve transection

Cited by 21 publications

References 42 publications

Fibrin matrices with affinity‐based delivery systems and neurotrophic factors promote functional nerve regeneration

Fibrin matrices with affinity‐based delivery systems and neurotrophic factors promote functional nerve regeneration

Affinity-based release of glial-derived neurotrophic factor from fibrin matrices enhances sciatic nerve regeneration

Improvement of peripheral nerve regeneration in acellular nerve grafts with local release of nerve growth factor

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