Extraordinary Transmission‐based Plasmonic Nanoarrays for Axially Super‐Resolved Cell Imaging

Abstract

Extraordinary transmission based axial imaging (EOT-AIM) for cell microscopy is reported. EOT-AIM uses linear arrays of nanoapertures, each of which samples target fl uorescence up to a preset axial distance from surface, in combination with wide-fi eld microscopy for acquisition of lateral images. Current design of nanoapertures provides EOT-AIM with axial super-resolution that is as small as 20 nm for a depth range of 500 nm. Experiments were performed for the measurement of the axial distribution of ganglioside in mouse macrophage (RAW264.7) cells using FITC-conjugated cholera toxin subunit B. The results were successfully confi rmed with conventional confocal and total internal refl ection fl uorescence microscopy. Adv. Optical Mater. 2014, 2, 48-55 49 wileyonlinelibrary.com

Extraordinary transmission-based super-resolved axial imaging using subwavelength metallic nanoaperture arrays

“…Finally, the axial distance information of ganglioside was distinguished over a depth range of 500 nm with a resolution between 20 and 125 nm. The results were confirmed at a reduced image resolution by conventional fluorescence imaging such as total internal reflection fluorescence and confocal microscopy [17]. …”

Nanostructured Color Filters: A Review of Recent Developments

“…The emergence of Extraordinary Optical Transmission (EOT) [9] due to coupling of surface plasmons in a surface lattice has been a breakthrough. It has been widely used and its applications include drug delivery [125], imaging [126,127], color filtering [128,129], high resolution display [130] and high sensitive sensing [131,132]. Since the research in the field of plasmonics is expedited with the discovery of new materials and design fabrication techniques [72,92,133], this review focuses on and evaluates those developments.…”

Surface-plasmon enhanced microscopy using blocked silver nanodot arrays

“…Also, structured illumination microscopy attained superresolution with modulation and reconstruction at carrier spatial frequency [4]. On the other hand, plasmonic nanostructures have been investigated for improved resolution with localized near-fields in the far field [5][6][7][8][9].…”