Extreme PCR: A Breakthrough Innovation for Outbreaks?

Kumar, Vikram Sheel; Webster, Molly

doi:10.1373/clinchem.2014.236950

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…However, by optimizing primer, enzyme, magnesium concentration and buffers and changing the classic PCR reaction vessels to microfluidics or to materials that allow for very rapid temperature changes, extreme PCR platforms have shown that reaction speeds of the enzymes were not the limiting factor [60]. In extreme PCR, the time required for a single traditional PCR cycle is reduced to less than one minute (Figure 3) and the complete set of PCR cycles can be reduced to less than 10 mins [60, 61]. …”

Section: 0 To Infinity and Beyond: New Methods On The Horizonmentioning

confidence: 99%

Molecular monitoring of chronic myeloid leukemia: present and future

Yeung

Egan

Radich

2016

Expert Review of Molecular Diagnostics

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Introduction Fusion of BCR-ABL1 genes causes chronic myeloid leukemia (CML). As a reliable marker of disease burden, it also serves as the target of tyrosine kinase inhibitors (TKIs). New more sensitive molecular diagnostic tools for BCR-ABL1 can contribute to therapeutic decision-making, especially in considering drug discontinuation for patients enjoying prolonged deep molecular response. Areas covered Several novel platforms are transforming CML molecular diagnostics to enable faster point-of-care devices, better understanding of clonal diversity and resistance mutations. Here, we review these molecular platforms, knowing implementation in other hematological malignancies will ensue. Expert commentary Treatment with TKI in CML is the first example of a highly effective targeted therapy. Monitoring of BCR-ABL1 mRNA is standard in assessing disease burden being highly predictive of outcomes recommended by both ELN and NCCN; however, studies has demonstrated poor adherence to these recommendations. In both clinical practice and assay performance, further optimizing of BCR-ABL1 monitoring can be envisioned including point-of-care methods for increased availability of rapid, standardized testing and increasingly sensitive molecular assays that allow for quantification of MRD and detecting resistance mutations.

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Section: 0 To Infinity and Beyond: New Methods On The Horizonmentioning

confidence: 99%

Molecular monitoring of chronic myeloid leukemia: present and future

Yeung

Egan

Radich

2016

Expert Review of Molecular Diagnostics

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“…Portable PCR techniques are currently under development and featured to be readily deployed in the field for rapid diagnosis (10–30 min). These techniques are anticipated to have minimum biosafety requirements and do not require laboratory infrastructure [ 67 ]. Portable PCR techniques can play a more effective role in disease surveillance and control including Ebola outbreaks and other infectious diseases [ 68 ].…”

Section: Diagnosis Of Ebola Virus Diseasementioning

confidence: 99%

Ebola Virus Infection: Overview and Update on Prevention and Treatment

et al. 2015

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In 2014 and 2015, the largest Ebola virus disease (EVD) outbreak in history affected large populations across West Africa. The goal of this report is to provide an update on the epidemic and review current progress in the development, evaluation and deployment of prevention and treatment strategies for EVD. Relevant information was identified through a comprehensive literature search using Medline, PubMed and CINAHL Complete and using the search terms Ebola, Ebola virus disease, Ebola hemorrhagic fever, West Africa outbreak, Ebola transmission, Ebola symptoms and signs, Ebola diagnosis, Ebola treatment, vaccines for Ebola and clinical trials on Ebola. Through 22 July 2015, a total of 27,741 EVD cases and 11,284 deaths were reported from all affected countries. Several therapeutic agents and novel vaccines for EVD have been developed and are now undergoing evaluation. Concurrent with active case investigation, contact tracing, surveillance and supportive care to patients and communities, there has been rapid progress in the development of new therapies and vaccines against EVD. Continued focus on strengthening clinical and public health infrastructure will have direct benefits in controlling the spread of EVD and will provide a strong foundation for deployment of new drugs and vaccines to affected countries when they become available. The unprecedented West Africa Ebola outbreak, response measures, and ensuing drug and vaccine development suggest that new tools for Ebola control may be available in the near future.Electronic supplementary materialThe online version of this article (doi:10.1007/s40121-015-0079-5) contains supplementary material, which is available to authorized users.

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PCR: Identification of Genetic Polymorphisms

Harbison

Nguyen

2017

Methods in Molecular Biology

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Polymerase chain reaction (PCR) enables the amplification of a specific sequence of deoxyribonucleic acid (DNA) through the process of three main steps: template DNA denaturation, annealing of the primers to complementary sequences, and primer extension to synthesize DNA strands. By using this method, the target sequence will be copied and amplified at an exponential rate. PCR provides a qualitative method for identifying DNA from fresh or dried cells/body fluids, formalin-fixed archival tissue specimens, and ancient specimens.Herein we describe basic information for performing successful PCR experiments using the amplification of a human Alu insertion on the PV92 gene locus on chromosome 16 as an example method.

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Extreme PCR: A Breakthrough Innovation for Outbreaks?

Cited by 6 publications

References 5 publications

Molecular monitoring of chronic myeloid leukemia: present and future

Molecular monitoring of chronic myeloid leukemia: present and future

Ebola Virus Infection: Overview and Update on Prevention and Treatment

PCR: Identification of Genetic Polymorphisms

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