Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells

Razavi, Shahnaz; Salimi, Marzieh; Shahbazi‐Gahrouei, Daryoush; Karbasi, Saeed; Kermani, Saeed

doi:10.4103/2277-9175.124668

Cited by 24 publications

(20 citation statements)

References 34 publications

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“…A critical issue that had to be taken into consideration in designing this in vitro study was to maximize the cell exposure to the field. In fact, several authors have shown that the electrical field depends on the cross-sectional area of the culture media seen by the magnetic field [ 27 – 29 ]. Therefore, in this study, the PST® treatment was performed by placing the cell culture plate at the center of the PST® device, parallel to the ground, in order to have the magnetic field vector perpendicular to the plate surface.…”

Section: Discussionmentioning

confidence: 99%

Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study

Randelli

Menon

Ragone

et al. 2016

BMC Complement Altern Med

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BackgroundCurrent clinical procedures for rotator cuff tears need to be improved, as a high rate of failure is still observed. Therefore, new approaches have been attempted to stimulate self-regeneration, including biophysical stimulation modalities, such as low-frequency pulsed electromagnetic fields, which are alternative and non-invasive methods that seem to produce satisfying therapeutic effects. While little is known about their mechanism of action, it has been speculated that they may act on resident stem cells. Thus, the purpose of this study was to evaluate the effects of a pulsed electromagnetic field (PST®) on human tendon stem cells (hTSCs) in order to elucidate the possible mechanism of the observed therapeutic effects.MethodshTSCs from the rotator cuff were isolated from tendon biopsies and cultured in vitro. Then, cells were exposed to a 1-h PST® treatment and compared to control untreated cells in terms of cell morphology, proliferation, viability, migration, and stem cell marker expression.ResultsExposure of hTSCs to PST® did not cause any significant changes in proliferation, viability, migration, and morphology. Instead, while stem cell marker expression significantly decreased in control cells during cell culturing, PST®-treated cells did not have a significant reduction of the same markers.ConclusionsWhile PST® did not have significant effects on hTSCs proliferation, the treatment had beneficial effects on stem cell marker expression, as treated cells maintained a higher expression of these markers during culturing. These results support the notion that PST® treatment may increase the patient stem cell regenerative potential.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1261-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study

Randelli

Menon

Ragone

et al. 2016

BMC Complement Altern Med

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“…hASCs proliferation and survival were enhanced by PEMFs treatment [52]. Moreover, in combination with chondrogenic inductive medium, PEMFs were able to increase ASCs chondrogenic differentiation, in terms of expression of Sox9, collagen type I and II, aggrecan and osteocalcin, as well as mineralized matrix, and glycosaminoglycans deposition [53].…”

Section: Pulsed Electromagnetic Fieldmentioning

confidence: 99%

Mesenchymal stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal disorders

et al. 2016

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BackgroundMusculoskeletal disorders are regarded as a major cause of worldwide morbidity and disability, and they result in huge costs for national health care systems. Traditional therapies frequently turned out to be poorly effective in treating bone, cartilage, and tendon disorders or joint degeneration. As a consequence, the development of novel biological therapies that can treat more effectively these conditions should be the highest priority in regenerative medicine.Main body of the abstractMesenchymal stem cells (MSCs) represent one of the most promising tools in musculoskeletal tissue regenerative medicine, thanks to their proliferation and differentiation potential and their immunomodulatory and trophic ability. Indeed, MSC-based approaches have been proposed for the treatment of almost all orthopedic conditions, starting from different cell sources, alone or in combination with scaffolds and growth factors, and in one-step or two-step procedures. While all these approaches would require cell harvesting and transplantation, the possibility to stimulate the endogenous MSCs to enhance their tissue homeostasis activity represents a less-invasive and cost-effective therapeutic strategy. Nowadays, the role of tissue-specific resident stem cells as possible therapeutic target in degenerative pathologies is underinvestigated. Biophysical stimulations, and in particular extracorporeal shock waves treatment and pulsed electromagnetic fields, are able to induce proliferation and support differentiation of MSCs from different origins and affect their paracrine production of growth factors and cytokines.Short conclusionsThe present review reports the attempts to exploit the resident stem cell potential in musculoskeletal pathologies, highlighting the role of MSCs as therapeutic target of currently applied biophysical treatments.

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“…According to recent literature, ELF‐MF exposure is believed to play a role in the later stages (promotion and progression) of tumorigenesis, especially in brain‐derived tumors (Poulletier de Gannes et al, ; Turner et al, ). Critical cellular pathways and functions have been proposed as targets of ELF magnetic fields: DNA damage profile (Panagopoulos et al, ; Villarini et al, ; Yoon et al, ), proliferation and/or survival dynamics (Martínez et al, ; Trillo et al, ; Razavi et al, ), signaling pathways (Nie and Henderson, ; Patruno et al, ), and redox balance (Falone et al, ; Falone et al, ; Di Loreto et al, ; Mattsson and Simkó, ). We have previously shown that the exposure to an ELF magnetic field increases the proliferation of cells isolated from neuroblastoma, and this was linked to the novel expression of protein associated to a more malignant phenotype (Sulpizio et al, ).…”

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confidence: 99%

Improved Mitochondrial and Methylglyoxal‐Related Metabolisms Support Hyperproliferation Induced by 50 Hz Magnetic Field in Neuroblastoma Cells

Falone

Santini

Loreto

et al. 2016

Journal Cellular Physiology

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Extremely low frequency magnetic fields (ELF-MF) are common environmental agents that are suspected to promote later stages of tumorigenesis, especially in brain-derived malignancies. Even though ELF magnetic fields have been previously linked to increased proliferation in neuroblastoma cells, no previous work has studied whether ELF-MF exposure may change key biomolecular features, such as anti-glycative defence and energy re-programming, both of which are currently considered as crucial factors involved in the phenotype and progression of many malignancies. Our study investigated whether the hyperproliferation that is induced in SH-SY5Y human neuroblastoma cells by a 50 Hz, 1 mT ELF magnetic field is supported by an improved defense towards methylglyoxal (MG), which is an endogenous cancer-static and glycating α-oxoaldehyde, and by rewiring of energy metabolism. Our findings show that not only the ELF magnetic field interfered with the biology of neuron-derived malignant cells, by de-differentiating further the cellular phenotype and by increasing the proliferative activity, but also triggered cytoprotective mechanisms through the enhancement of the defense against MG, along with a more efficient management of metabolic energy, presumably to support the rapid cell outgrowth. Intriguingly, we also revealed that the MF-induced bioeffects took place after an initial imbalance of the cellular homeostasis, which most likely created a transient unstable milieu. The biochemical pathways and molecular targets revealed in this research could be exploited for future approaches aimed at limiting or suppressing the deleterious effects of ELF magnetic fields. J. Cell. Physiol. 231: 2014-2025, 2016. © 2016 Wiley Periodicals, Inc.

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Extremely low-frequency electromagnetic field influences the survival and proliferation effect of human adipose derived stem cells

Cited by 24 publications

References 34 publications

Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study

Effects of the pulsed electromagnetic field PST® on human tendon stem cells: a controlled laboratory study

Mesenchymal stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal disorders

Improved Mitochondrial and Methylglyoxal‐Related Metabolisms Support Hyperproliferation Induced by 50 Hz Magnetic Field in Neuroblastoma Cells

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