Extremely low frequency electromagnetic fields activate the ERK cascade, increase hsp70 protein levels and promote regeneration in Planaria

Goodman, Reba; Lin-Ye, Avary; Geddis, Matthew; Wickramaratne, Priya; Hodge, Susan E.; Pantazatos, Spiro P.; Blank, Martin; Ambron, Richard T.

doi:10.3109/09553000903072488

Cited by 35 publications

(21 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…45,46 Mechanical and sonic forces are also generated by DBD plasmas. 21 Each of these modalities, applied in one way or another, have been reported to enhance differentiation of skeletal tissues 47 and cannot be ruled out as causative factors. Further study is needed to determine any role these other effects play in limb bud development.…”

Section: Discussionmentioning

confidence: 99%

Nonthermal Atmospheric Pressure Plasma Enhances Mouse Limb Bud Survival, Growth, and Elongation

Chernets

Zhang

Steinbeck

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

The enhanced differentiation of mesenchymal cells into chondrocytes or osteoblasts is of paramount importance in tissue engineering and regenerative therapies. A newly emerging body of evidence demonstrates that appendage regeneration is dependent on reactive oxygen species (ROS) production and signaling. Thus, we hypothesized that mesenchymal cell stimulation by nonthermal (NT)-plasma, which produces and induces ROS, would (1) promote skeletal cell differentiation and (2) limb autopod development. Stimulation with a single treatment of NT-plasma enhanced survival, growth, and elongation of mouse limb autopods in an in vitro organ culture system. Noticeable changes included enhanced development of digit length and definition of digit separation. These changes were coordinated with enhanced Wnt signaling in the distal apical epidermal ridge (AER) and presumptive joint regions. Autopod development continued to advance for approximately 144 h in culture, seemingly overcoming the negative culture environment usually observed in this in vitro system. Realtime quantitative polymerase chain reaction analysis confirmed the up-regulation of chondrogenic transcripts. Mechanistically, NT-plasma increased the number of ROS positive cells in the dorsal epithelium, mesenchyme, and the distal tip of each phalange behind the AER, determined using dihydrorhodamine. The importance of ROS production/signaling during development was further demonstrated by the stunting of digital outgrowth when anti-oxidants were applied. Results of this study show NT-plasma initiated and amplified ROS intracellular signaling to enhance development of the autopod. Parallels between development and regeneration suggest that the potential use of NT-plasma could extend to both tissue engineering and clinical applications to enhance fracture healing, trauma repair, and bone fusion.

show abstract

Section: Discussionmentioning

confidence: 99%

Nonthermal Atmospheric Pressure Plasma Enhances Mouse Limb Bud Survival, Growth, and Elongation

Chernets

Zhang

Steinbeck

et al. 2015

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…Recently; several studies have been performed to verify direct effects exerted by ELF-EMFs on living cells functions. Although the results have been somewhat controversial, a variety of cell responses have been observed involving proliferation and differentiation [1][2][3], gene expression [4][5][6], modulation of the membrane receptors functionality [7,8] and free radicals' generation [9][10][11]. In 2002, the IARC classified ELF-EMFs as a "possible human carcinogen" and the decision IARC was supported by the WHO in 2007 [12].…”

Section: Introductionmentioning

confidence: 99%

Relative biophysical effects on rat's bone as result of high energy photons and magnetic fields exposures

Khalil

2019

Arab Journal of Nuclear Sciences and Applications

View full text Add to dashboard Cite

There is critical need to do a lot of experimental studies to calculate of the relative biological effects of non-ionizing radiation and comparing its effect with ionizing radiation to re-determine the safe exposure dose to non-ionizing radiation. This research is concerned with studying the relative fraction of damage (RFD) of the extremely low frequency electromagnetic fields (4kV/m-50 Hz) to high energy photons (6 MeV) on some biophysical properties of rats bone. In this regard, 100 male albino rats are used and divided into sub groups of 10 rats each. One group of animals was used as control group (unexposed group) and did not receive any treatment. Similar to occupational workers, four groups of animals were exposed 8h/day for periods from 1 to 4 weeks and were immediately sacrificed at the end of the exposure period. Four groups of animals exposed 8h/day for periods from 1 to 4 weeks and remained 45 days post exposure periods, then sacrificed. The last group of animals was exposed to a high energy dose of6 MeV of dose (1rad). Femur bone samples of each animal from all groups were collected and prepared for further biophysical investigations. In addition, RFD of magnetic fields exposures are calculated for each group relative to dose of 6 MeV-1 rad photons. Results of exposure to the electric field showed significant deterioration effect on the biomechanical parameters of femurs as a consequential defect of bone strength and bone quality as compared to the unexposed ones. Also, the results obtained from the groups that udergone a post exposure period of 45-days did not show amendment in bone biophysical characteristics, which means that permanent effects and chronic damage of bone tissues may have resulted. On the other hand, the RFD ratios illustrated that the effect of exposure to ELF EMF has no lower effect than the exposure to ionizing radiation and should not be underestimated.

show abstract

“…The ELF-EMFs have been found to produce a variety of biological effects, from simple enzyme reactions to the far more complex gene induction and protein [Goodman and Blank, 1998;De Mattei et al, 2005;Delle Monache et al, 2008;Piacentini et al, 2008;Goodman et al, 2009]. Recently, studies carried out on mammalian sperm suggest that ELF-EMFs can negatively [Bernabò et al, 2007] or positively [Iorio et al, 2007;Roychoudhury et al, 2009] influence sperm motility according to the specific characteristics of the EMF applied and/or to the type of sperm used.…”

Section: Introductionmentioning

confidence: 99%

Involvement of mitochondrial activity in mediating ELF‐EMF stimulatory effect on human sperm motility

Iorio

Monache

Bennato

et al. 2010

Bioelectromagnetics

View full text Add to dashboard Cite

It has recently been reported that the exposure of human spermatozoa to an extremely low frequency (ELF) electromagnetic field (EMF) with a square waveform of 5 mT amplitude and frequency of 50 Hz improves sperm motility. The functional relationship between the energy metabolism and the enhancement of human sperm motility induced by ELF-EMF was investigated. Sperm exposure to ELF-EMF resulted in a progressive and significant increase of mitochondrial membrane potential and levels of ATP, ADP and NAD(+) that was associated with a progressive and significant increase in the sperm kinematic parameters. No significant effects were detected on other parameters such as ATP/ADP ratio and energy charge. When carbamoyl cyanide m-chlorophenylhydrazone (CICCP) was applied to inhibit the oxidative phosphorylation in the mitochondria, the values of energy parameters and motility in the sperm incubated in the presence of glucose and exposed to ELF-EMF did not change, thus indicating that the glycolysis was not involved in mediating ELF-EMF stimulatory effect on motility. By contrast, when pyruvate and lactate were provided instead of glucose, the energy status and motility increased significantly in ELF-EMF-treated sperm. Under these culture conditions, the inhibition of glycolitic metabolism by 2-deoxy-D-glucose (DOG) again resulted in increased values of energy and kinematic parameters, indicating that gluconeogenesis was not involved in producing glucose for use in glycolysis. We concluded that the key role in mediating the stimulatory effects exerted by ELF-EMF on human sperm motility is played by mitochondrial oxidative phosphorylation rather than glycolysis.

show abstract

Extremely low frequency electromagnetic fields activate the ERK cascade, increase hsp70 protein levels and promote regeneration in Planaria

Cited by 35 publications

References 44 publications

Nonthermal Atmospheric Pressure Plasma Enhances Mouse Limb Bud Survival, Growth, and Elongation

Nonthermal Atmospheric Pressure Plasma Enhances Mouse Limb Bud Survival, Growth, and Elongation

Relative biophysical effects on rat's bone as result of high energy photons and magnetic fields exposures

Involvement of mitochondrial activity in mediating ELF‐EMF stimulatory effect on human sperm motility

Contact Info

Product

Resources

About