Fabric phase sorptive extraction for the isolation of five common antidepressants from human urine prior to HPLC-DAD analysis

Lioupi, Artemis; Kabir, Abuzar; Furton, Kenneth G.; Samanidou, Victoria

doi:10.1016/j.jchromb.2019.04.045

Cited by 56 publications

(21 citation statements)

References 50 publications

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“…The validations for simultaneous determination of antidepressants are performed in chromatographic columns, mostly C18 2019;LIOUPI et al;2019;PAIGA et al;2017). This study demonstrates the possibility of using a C8 column.…”

Section: Validationmentioning

confidence: 84%

Optimization of a methodology for simultaneous determination of four antidepressants present in fresh water by high efficiency liquid chromatography

Evagelista

Vilca

Pimpinato

et al. 2020

REGET

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The occurrence of emerging contaminants is a growing concern in the environmental scenario due to their potential risks to ecosystems. The technologies employed in the treatment of sewage in Brazil are not efficient in removing micropollutants, especially persistent ones. Antidepressants (a class of drugs belonging to emerging contaminants) can reach the environment through the disposal of domestic and industrial effluents. These substances were detected in studies with surface waters, being able to cause changes and accumulate in aquatic organisms. The occurrence and impacts of this class of pollutant are still poorly studied in the country. Thus, there is a need to carry out environmental monitoring. To meet such a goal, scientific advances must be developed, especially those related to the development of analytical skills, equipment, and methods with the necessary sensitivity to research in the different sources of contamination. Accordingly, with the development of analytical techniques, it is possible to determine antidepressant drugs in environmental and / or biological matrices in increasingly small concentrations. Consequently, optimization of current techniques and proposing new ones are crucial before any other actions. The aim of this study was to validate a rapid methodology for determining the presence of four antidepressants - fluoxetine, citalopram, venlafaxine, and sertraline - in fresh water through high performance liquid chromatography. The method involved solid-phase extraction (SPE) with C18 cartridge and quantification by high performance liquid chromatography coupled to a diode array detector with C8 column. No chromatographic interference was observed in the retention time of the antidepressants in this study at the selected wavelength of 235 nm. The study matrix did not interfere in the analyses. Linearity was adequate for the range from 0.5 to 10 µg mL-1, with limits of detection and quantification of 0.03 to 0.09 µg mL-1 and 0.10 to 0.27 µg mL-1 respectively. Accuracy was assessed by testing sample fortification at three concentration levels and estimated by relative standard deviation (RSD). RSD values below 15% were obtained. The recovery interval ranged from 49 to 102%. The analytical method was validated and considered satisfactory for the simultaneous determination of antidepressants in freshwater samples using a C8 column.

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Section: Validationmentioning

confidence: 84%

Optimization of a methodology for simultaneous determination of four antidepressants present in fresh water by high efficiency liquid chromatography

Evagelista

Vilca

Pimpinato

et al. 2020

REGET

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“…Then, it was evaporated until dryness and reconstituted with 50 μl of acetonitrile–methanol (80:20 v/v), prior to injection into the HPLC system, with a view to achieving preconcentration of the analytes. The FPSE medium was then washed with 2 ml of ACN–MeOH (50:50 v/v) in order to be reused up to 30 times without any considerable change in the extraction performance, when an arbitrary 10% recovery loss criterion was adopted, as evidenced in previous research involving the same FPSE membrane (Lioupi et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

“…The FPSE medium was then washed with 2 ml of ACN-MeOH (50:50 v/v) in order to be reused up to 30 times without any considerable change in the extraction performance, when an arbitrary 10% recovery loss criterion was adopted, as evidenced in previous research involving the same FPSE membrane (Lioupi et al, 2019).…”

Section: Fpsementioning

confidence: 99%

“…The FPSE membranes are flexible and permeable and possess a large primary contact surface area, which leads to rapid analyte extraction based on the equilibrium extraction mode (Aznar, Alfaro, Nerin, Kabir, & Furton, 2016; Kabir, Mesa, Jurmain, & Furton, 2017; Zilfidou, Kabir, Furton, & Samanidou, 2018). FPSE has been successfully applied to the pretreatment of many types of samples such as biological, environmental, food, pharmaceutical and toxicological combined with a variety of analytical techniques such as for example HPLC coupled with diode array detection (DAD) (Alampanos, Kabir, Furton, Samanidou, & Papadoyannis, 2019; Lioupi, Kabir, Furton, & Samanidou, 2019; Zilfidou, Kabir, Furton, & Samanidou, 2019), UHPLC–MS (Pérez‐Mayán et al, 2019; Santana‐Viera et al, 2017) and GC–MS (Alcudia‐León et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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An improved fabric‐phase sorptive extraction protocol for the determination of seven parabens in human urine by HPLC–DAD

Rigkos

Alampanos

Kabir

et al. 2020

Biomedical Chromatography

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An improved fabric‐phase sorptive extraction (FPSE) protocol has been developed and validated herein for the simple, fast, sensitive and green determination of seven parabens—methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isopropyl paraben, isobutyl paraben and benzyl paraben—in human urine samples by HPLC–DAD. The mobile phase consisted of ammonium acetate (0.05 m) and acetonitrile, while total analysis time was 13.2 min. Sol–gel poly (tetrahydrofuran) coated FPSE membrane resulted in optimum extraction sensitivity for the seven parabens. The novel FPSE medium as well as the improved and faster sample preparation procedure resulted in lower limit of detection and quantitation values in comparison with previously reported methods. The separation was carried out using an RP‐HPLC method with a Spherisorb C18 column and a flow rate of 1.4 ml/min. The validation of the analytical method was carried out by means of linearity, precision, accuracy, selectivity, sensitivity and robustness. For all seven parabens, the limits of detection and quantitation were 0.003 and 0.01 μg/ml, respectively. Relative recovery rates were between 86.3 and 104%, while RSD values were <12.6 and 19.3% for within‐ and between‐day repeatability, respectively. The method was subsequently applied to real human urine samples.

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“…In 2019, Lioupi et al [ 40 ] developed and validated an innovative fabric phase sorptive extraction high-performance liquid chromatography–diode array detection (FPSE-HPLC-DAD) method for the extraction of five common antidepressants (venlafaxine, paroxetine, fluoxetine, amitriptyline, clomipramine) in human urine samples. The extraction protocol was optimized with regards to the extraction main parameters.…”

Section: Applicationsmentioning

confidence: 99%

Fabric Phase Sorptive Extraction: A Paradigm Shift Approach in Analytical and Bioanalytical Sample Preparation

Kabir

Samanidou

2021

Molecules

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Fabric phase sorptive extraction (FPSE) is an evolutionary sample preparation approach which was introduced in 2014, meeting all green analytical chemistry (GAC) requirements by implementing a natural or synthetic permeable and flexible fabric substrate to host a chemically coated sol–gel organic–inorganic hybrid sorbent in the form of an ultra-thin coating. This construction results in a versatile, fast, and sensitive micro-extraction device. The user-friendly FPSE membrane allows direct extraction of analytes with no sample modification, thus eliminating/minimizing the sample pre-treatment steps, which are not only time consuming, but are also considered the primary source of major analyte loss. Sol–gel sorbent-coated FPSE membranes possess high chemical, solvent, and thermal stability due to the strong covalent bonding between the fabric substrate and the sol–gel sorbent coating. Subsequent to the extraction on FPSE membrane, a wide range of organic solvents can be used in a small volume to exhaustively back-extract the analytes after FPSE process, leading to a high preconcentration factor. In most cases, no solvent evaporation and sample reconstitution are necessary. In addition to the extensive simplification of the sample preparation workflow, FPSE has also innovatively combined the extraction principle of two major, yet competing sample preparation techniques: solid phase extraction (SPE) with its characteristic exhaustive extraction, and solid phase microextraction (SPME) with its characteristic equilibrium driven extraction mechanism. Furthermore, FPSE has offered the most comprehensive cache of sorbent chemistry by successfully combining almost all of the sorbents traditionally used exclusively in either SPE or in SPME. FPSE is the first sample preparation technique to exploit the substrate surface chemistry that complements the overall selectivity and the extraction efficiency of the device. As such, FPSE indeed represents a paradigm shift approach in analytical/bioanalytical sample preparation. Furthermore, an FPSE membrane can be used as an SPME fiber or as an SPE disk for sample preparation, owing to its special geometric advantage. So far, FPSE has overwhelmingly attracted the interest of the separation scientist community, and many analytical scientists have been developing new methodologies by implementing this cutting-edge technique for the extraction and determination of many analytes at their trace and ultra-trace level concentrations in environmental samples as well as in food, pharmaceutical, and biological samples. FPSE offers a total sample preparation solution by providing neutral, cation exchanger, anion exchanger, mixed mode cation exchanger, mixed mode anion exchanger, zwitterionic, and mixed mode zwitterionic sorbents to deal with any analyte regardless of its polarity, ionic state, or the sample matrix where it resides. Herein we present the theoretical background, synthesis, mechanisms of extraction and desorption, the types of sorbents, and the main applications of FPSE so far according to different sample categories, and to briefly show the progress, advantages, and the main principles of the proposed technique.

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Fabric phase sorptive extraction for the isolation of five common antidepressants from human urine prior to HPLC-DAD analysis

Cited by 56 publications

References 50 publications

Optimization of a methodology for simultaneous determination of four antidepressants present in fresh water by high efficiency liquid chromatography

Optimization of a methodology for simultaneous determination of four antidepressants present in fresh water by high efficiency liquid chromatography

An improved fabric‐phase sorptive extraction protocol for the determination of seven parabens in human urine by HPLC–DAD

Fabric Phase Sorptive Extraction: A Paradigm Shift Approach in Analytical and Bioanalytical Sample Preparation

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