Fabric phase sorptive extraction-high performance liquid chromatography-photo diode array detection method for simultaneous monitoring of three inflammatory bowel disease treatment drugs in whole blood, plasma and urine

Kabir, Abuzar; Furton, Kenneth G.; Tinari, Nicola; Grossi, Laurino; Innosa, Denise; Macerola, Daniela; Tartaglia, Angela; Donato, Valentina Di; D’Ovidio, Cristian; Locatelli, Marcello

doi:10.1016/j.jchromb.2018.03.028

Cited by 63 publications

(28 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this work, following our previous work on whole blood and non-conventional matrices [16][17][18][19][20][21] was validated a new, fast, high-throughput, and reproducible GC-FID assay for the evaluation of the ethanol concentration in blood and vitreous humor. The purpose was to highlight that the new method is a valid and validated alternative to other procedures, confirming the correlation between these different specimens, as reported in literature [22][23][24][25], that allow estimating blood alcohol concentrations when blood is unavailable or contaminated or in cases where blood analysis cannot be carried out.…”

Section: Introductionmentioning

confidence: 99%

Ethanol Determination in Post-Mortem Samples: Correlation between Blood and Vitreous Humor Concentration

et al. 2020

Self Cite

View full text Add to dashboard Cite

Ethanol (ethylic alcohol) represents the most commonly used drug worldwide and is often involved in clinical and forensic toxicology. Based on several reports, excessive alcohol consumption is the main contributing factor in traffic accidents, drownings, suicides, and other crimes. For these reasons, it becomes essential to analyze the alcohol concentration during autopsy. Although blood is usually used for alcohol analysis in post-mortem cases, it could suffer alterations, putrefaction, and microbial contaminations. As an alternative to whole blood, vitreous humor has been successfully used in medico-legal studies. In this work, post-mortem specimens were analyzed for ethanol determination. The analysis of blood and vitreous humor were carried-out using gas chromatography-flame ionized detector (GC-FID) with a total run time of 6 min. The method was validated in terms of limit of detection, limit of quantification, dynamic range, sensibility, recovery, precision and trueness. A linear regression analysis indicated a coefficient of determination (R2) of 0.9981. The study confirmed no statistically differences between alcohol concentration in blood and vitreous humor, leading vitreous humor as an excellent matrix that could be used as an alternative to whole blood in toxicological analysis in cases where blood is not available.

show abstract

Section: Introductionmentioning

confidence: 99%

Ethanol Determination in Post-Mortem Samples: Correlation between Blood and Vitreous Humor Concentration

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The modified fabric is directly submerged into the sample containing the target analytes and, once equilibrium is reached, the analytes retained on the extraction medium can be eluted with appropriate organic solvent [44]. This technique ensures high retention capacity through higher sorbent loading, fast extraction interaction, relatively short extraction time and reduced consumption of organic solvents in the sample preparation [45][46][47].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Efficiency of UHPLC-Orbitrap MS for the Determination of 20 Pharmaceuticals and Acesulfame K in Hospital and Urban Wastewaters with the Aid of FPSE

Kalaboka¹,

Chrimatopoulos²,

Jiménez‐Holgado³

et al. 2020

Separations

View full text Add to dashboard Cite

Aside from the classical residues of persistent organic pollutants (POPs), the occurrence of emerging contaminants (ECs) in the environment has become a subject of increasing concern due to their harmful impact on the aquatic environment. Wastewater treatment plant (WWTP) effluents are major sources of environmental pollution. Therefore, data concerning their existence is required. In this study, twenty compounds representative of different drug groups considered ECs and belonging to antibiotics, antipsychotics, anti-inflammatory drugs plus acesulfame K were selected to be accurately detected and quantified with UHPLC–LTQ-Orbitrap MS in hospital and urban WWTP effluents. Chromatographic parameters (column efficiency, mobile phase, etc.), as well as mass spectrometry conditions concerning ionization mode and Orbitrap analysis (ESI options, mass resolving power, AGC target, tube lens, injection time), were evaluated. Moreover, a novel fabric phase sorptive extraction (FPSE) method based on fiber glass coated with PEG300 was employed as sample preparation process. Experimental parameters affecting extraction and desorption steps such as sample pH, extraction time, ionic strength, elution time and solvent have been optimized. The optimized methodology was validated providing excellent linearity (R2 > 0.99), and low detection and quantification limits up to 3.1 and 9.3 ng/L, for carbamazepine, respectively. Relative recoveries ranged from 81.1% to 114.0%, while a medium matrix effect for most of the target compounds occurred. Applying the above analytical method in effluents of WWTPs from NW Greece, nine compounds were quantified with concentrations that varied from 55.4 to 728.4 ng/L.

show abstract

“…Among different treatment regimens used in inflammatory bowel disease (IBD), cortisone, ciprofloxacin, and/or sulfasalazine are orally administrated among other drugs; these drugs are generally present at trace level concentrations in physiological samples including plasma, urine, and saliva. As such, a preconcentration step is necessary to obtain advantageous signal-to-noise ratios in chromatographic separation and detection [6,7]. The logP values of the target analytes ranging from 1.66 for cortisone, to 2.30 for ciprofloxacin, and 2.92 for sulfasalazine (Table 1), coupled to their widespread range of pK a values pose a great challenge for their simultaneous extraction and analysis.…”

Section: Introductionmentioning

confidence: 99%

Comparison between Exhaustive and Equilibrium Extraction Using Different SPE Sorbents and Sol-Gel Carbowax 20M Coated FPSE Media

et al. 2019

Self Cite

View full text Add to dashboard Cite

This paper reports the performance comparison between the exhaustive and equilibrium extraction using classical Avantor C18 solid phase extraction (SPE) sorbent, hydrophilic-lipophilic balance (HLB) SPE sorbent, Sep-Pak C18 SPE sorbent, novel sol-gel Carbowax 20M (sol-gel CW 20M) SPE sorbent, and sol-gel CW 20M coated fabric phase sorptive extraction (FPSE) media for the simultaneous extraction and analysis of three inflammatory bowel disease (IBD) drugs that possess logP values (polarity) ranging from 1.66 for cortisone, 2.30 for ciprofloxacin, and 2.92 for sulfasalazine. Both the commercial SPE phases and in-house synthesized sol-gel CW 20M SPE phases were loaded in SPE cartridges and the extractions were carried out under an exhaustive extraction mode. FPSE was carried out under an equilibrium extraction mode. The drug compounds were resolved using a Luna C18 column (250 mm × 4.6 mm; 5 μm particle size) in gradient elution mode within 20 min and the method was validated in compliance with international guidelines for the bioanalytical method validation. Novel in-house synthesized and loaded sol-gel CW 20M SPE sorbent cartridges were characterized in terms of their extraction capability, breakthrough volume, retention volume, hold-up volume, number of the theoretical plate, and the retention factor.

show abstract

Fabric phase sorptive extraction-high performance liquid chromatography-photo diode array detection method for simultaneous monitoring of three inflammatory bowel disease treatment drugs in whole blood, plasma and urine

Cited by 63 publications

References 37 publications

Ethanol Determination in Post-Mortem Samples: Correlation between Blood and Vitreous Humor Concentration

Ethanol Determination in Post-Mortem Samples: Correlation between Blood and Vitreous Humor Concentration

Exploring the Efficiency of UHPLC-Orbitrap MS for the Determination of 20 Pharmaceuticals and Acesulfame K in Hospital and Urban Wastewaters with the Aid of FPSE

Comparison between Exhaustive and Equilibrium Extraction Using Different SPE Sorbents and Sol-Gel Carbowax 20M Coated FPSE Media

Contact Info

Product

Resources

About