Fabrication and investigation potential effect of lentinan and docetaxel nanofibers for synergistic treatment of breast cancer in vitro

Abstract

Breast cancer has a high prevalence among women which has high recurrences even after common therapies, so using nanofibers that contain natural compounds along with antineoplastic drugs locoregionally can be a novel supportive strategy. In this study, hot water extracted lentinan from Lentinula edodes (TMU340) was purified by ion-exchange chromatography. The effects of electrospun PVA/lentinan/docetaxel nanofibers were determined on HER3 expression in MCF-7 cell line. Lentinan concentration was determined at 0.243 mg/ml. The optimal electrospinning conditions were 15 kV, 15 cm, and 1 ml/h. The HER3 expression of cells treated with PVA/lentinan/docetaxel nanofibers obtained 0.52 in the most effective concentration at 72 h. Based on the results, PVA/lentinan/docetaxel nanofibers have shown appropriate synergistic effects on breast cancer. Therefore, the utilization of lentinan as a natural compound with a common chemical anticancer drug and nano-drug delivery system can be considered as a promising suggestion within the treatment of cancer.

Tissue Engineering Scaffolds Loaded With a Variety of Plant Extracts: Novel Model in Breast Cancer Therapy

“… 62 Research has demonstrated that extract-loaded NFs are effective at causing cytotoxicity to BC cells. 66 These NFs’ controlled release method offers a possible means of lowering systemic toxicity and tumor recurrence after surgery. 39 In a similar vein, using piperine (PIP)-PCL75-Coll25 NFs in a 4T1 BC mouse model resulted in a tumor growth suppression that was effective and demonstrated the NFs’ sustained-release capabilities and strong anticancer effectiveness.…”

“… 62 On the contrary, not much is said about how patients receive these scaffolds inserted or how the body reacts to the procedure. 39 , 66 They emphasize how effective drug encapsulation and regulated release can reduce systemic toxicity and the chance of tumor recurrence. 65 However, a complete understanding of the practical implications of the medicines is hampered by the lack of data demonstrating clinical outcomes or patient reactions following therapy.…”

“…One prominent example of this integration is the utilization of plant extract–loaded scaffolds in BC models. 66 The inclusion of plant extracts, such as α-mangostin , in scaffolds designed for BC research offers several distinct advantages that contribute to their potential as a breakthrough in cancer therapy. 73 …”

“… 74 Triple NFs loaded with polyvinyl alcohol (PVA)/lentinan/docetaxel significantly reduce the viability of human BC cells and down-regulate the expression of the HER3 gene, a member of the BC signaling pathway. 66 …”

Medicinal Mushrooms: Their Bioactive Components, Nutritional Value and Application in Functional Food Production—A Review

“…Lentinan enhances the production of T lymphocytes and can potentiate the effect of AZT (3′-Azido-3′-deoxythymidine) in the anti-viral treatment of AIDS [ 27 ]. Its positive effects have been proved in the treatment of, e.g., glioma (human astrocytoma U251 cells) [ 46 ], breast cancer [ 47 ] and liver cancer [ 48 ]. In turn, the Turkey Tail ( Trametes versicolor (L.) Lloyd) contains characteristic proteoglucans.…”