Fabrication of circular microfluidic network in enzymatically-crosslinked gelatin hydrogel

He, Jiankang; Chen, Ruomeng; Lu, Yongjie; Li, Zhan; Liu, Yaxiong; Li, Dichen; Zhang, Jin

doi:10.1016/j.msec.2015.09.104

Cited by 67 publications

(51 citation statements)

References 38 publications

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“…Gelatin is low‐cost, and its biocompatibility has been proved repeatedly as it is the denatured product of collagen. [ 40,43 ] PLA and PCL are well‐known biomaterials widely used in tissue engineering applications mostly due to their mechanical properties, although their hydrophobic nature limits their application. [ 44,45 ] Just the opposite, gel presents many integrin biddings that are essential to promote cell adhesion and proliferation, though it has poor mechanical properties.…”

Section: Resultsmentioning

confidence: 99%

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Liu

Zhang

et al. 2020

Macro Materials & Eng

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Skin injuries are an urgent health issue, which raises a great concern in the clinic. Although numerous strategies have been proposed to fabricate skin substitutes for treatment of wounds over the past several decades, fabricating an ideal skin substitute to replace the damaged one can still be a problem. In this study, a novel biomimetic 3D composite skin scaffold is fabricated by combining electrohydrodynamic (EHD) jetting, electrospinning, and coating techniques. Here, the first polycaprolactone (PCL) porous structure is produced by the EHD jetting. Next, the second polylactic acid (PLA) membrane consisted of nanoscale fibers is prepared on the PCL porous structure via the electrospinning. The PCL porous structure and PLA fibers membrane can mimic the dermis and epidermis layer, respectively. Furthermore, gelatin is used as coating solution to enhance the biocompatibility of the scaffold. The structure and morphology of the fabricated scaffolds are analyzed, and the mechanical properties are investigated as well. Moreover, the in vitro and in vivo experiments demonstrate the biocompatibility of the materials and the fabrication process. In conclusion, these results demonstrate that the composite scaffold is effective and holds great potential for skin regeneration in the clinic.

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Section: Resultsmentioning

confidence: 99%

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Liu

Zhang

et al. 2020

Macro Materials & Eng

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“…The gelation has good biocompatibility and was used to fabricated issue scaffold repeatedly due to it is the denatured product of collagen . In addition, it showed comparable biological properties with collagen to support endothelial cells spreading and growth whether in vitro or in vivo studies . Enzymatically crosslinked gelatin has also been widely used in tissue engineering as a facile approach .…”

Section: Resultsmentioning

confidence: 99%

A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Liu

Zhang

et al. 2019

Macro Materials & Eng

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Despite the fact that the field of tissue engineering has had considerable advances over the past two decades, a series of unsolved problems still remain. Vascularization is one of the most important factors that greatly influence the function and size of the engineered scaffolds, which limits the clinical applications. In this work, a facile extracted molding method is presented for fabricating bulk tissue scaffolds with spatial networks. Briefly, the branched templates are designed, coated with paraffin on the surface, immersed into the mixture of microbial transglutaminase and gelatin, and extracted from fully enzymatic cross‐linking gelatin. The perfusion test is done and the mechanical properties of the scaffolds are investigated. Furthermore, in vitro and in vivo experiments demonstrate the nontoxicity and biocompatibility of the materials and fabrication process. Thus, this approach has great potential to overcome the challenge of rapid oxygen and nutrient delivery to engineered vascularized tissues implanted in vivo, opening the way to clinical applications.

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“…These templates are subsequently removed via external stimuli, resulting in an immediate formation of organized microchannels. However, most of these methods are limited by complicated postprocessing and the inability of complex fluidic network construction …”

Section: Introductionmentioning

confidence: 99%

“…In addition, multilayer microchannel structures would be easily obtained by stacking hydrogel sheets layer by layer . A set of strategies have been applied for the bonding of hydrogel sheets, such as mechanical bonding by pressure, and chemical bonding with the introduction of bonding reagents . However, hydrogel materials' hydrointerface causes bonding difficulty, and the bonding of hydrogel sheets generally calls for specific chemical reagents or physical conditions which can be adapted to specific materials only.…”

Section: Introductionmentioning

confidence: 99%

Vessel‐on‐a‐chip with Hydrogel‐based Microfluidics

Nie

Gao

Wang

et al. 2018

Small

139

102

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Hydrogel structures equipped with internal microchannels offer more in vivo‐relevant models for construction of tissues and organs in vitro. However, currently used microfabrication methods of constructing microfluidic devices are not suitable for the handling of hydrogel. This study presents a novel method of fabricating hydrogel‐based microfluidic chips by combining the casting and bonding processes. A twice cross‐linking strategy is designed to obtain a bonding interface that has the same strength with the hydrogel bulk, which can be applied to arbitrary combinations of hydrogels. It is convenient to achieve the construction of hydrogel structures with channels in branched, spiral, serpentine, and multilayer forms. The experimental results show that the combination of gelatin and gelatin methacrylate (GelMA) owns the best biocompatibility and can promote cell functionalization. Based on these, a vessel‐on‐a‐chip system with vascular function in both physiological and pathological situations is established, providing a promising model for further investigations such as vascularization, vascular inflammation, tissue engineering, and drug development. Taken together, a facile and cytocompatible approach is developed for engineering a user‐defined hydrogel‐based chip that can be potentially useful in developing vascularized tissue or organ models.

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Fabrication of circular microfluidic network in enzymatically-crosslinked gelatin hydrogel

Cited by 67 publications

References 38 publications

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

Development and Evaluation of Biomimetic 3D Coated Composite Scaffold for Application as Skin Substitutes

A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Vessel‐on‐a‐chip with Hydrogel‐based Microfluidics

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