Fabrication of Growth Factor Array Using an Inkjet Printer

Watanabe, Kohei; Fujiyama, Tomoyo; Mitsutake, Rina; Watanabe, Megumi; Tazaki, Yukiko; Miyazaki, Takeshi; Matsuda, Reiko

doi:10.1007/978-90-481-9145-1_12

Cited by 3 publications

(3 citation statements)

References 45 publications

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“…This study also examined the bioprinting rheology along with the underlying physics [61] and is considered as a cor nerstone in efficient fabrication of viable 3D vascular constructs with complex anatomies and vascular trees. Inkjet-based bioprint ing technology has also been used to print various biological com pounds, such as nucleic acid, proteins [62], growth factors [63,64] and biological cells [28,[31][32][33]35,65], Despite the great progress in inkjet-based bioprinting, this tech nique still faces some limitations. One of the main restrictions is the low upper limit for viscosity of bioink, which is on the order of 0.1 Pa s" 1 [66].…”

Section: Fig 1 Classification Of Bioprinting Techniquesmentioning

confidence: 99%

Bioprinting Technology: A Current State-of-the-Art Review

Dababneh

Özbolat

2014

Journal of Manufacturing Science and Engineering

371

287

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Bioprinting is an emerging technology for constructing and fabricating artificial tissue and organ constructs. This technology surpasses the traditional scaffold fabrication approach in tissue engineering (TE). Currently, there is a plethora of research being done on bioprinting technology and its potential as a future source for implants and full organ transplantation. This review paper overviews the current state of the art in bioprinting technology, describing the broad range of bioprinters and bioink used in preclinical studies. Distinctions between laser-, extrusion-, and inkjet-based bioprinting technologies along with appropriate and recommended bioinks are discussed. In addition, the current state of the art in bioprinter technology is reviewed with a focus on the commercial point of view. Current challenges and limitations are highlighted, and future directions for next-generation bioprinting technology are also presented.

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Section: Fig 1 Classification Of Bioprinting Techniquesmentioning

confidence: 99%

Bioprinting Technology: A Current State-of-the-Art Review

Dababneh

Özbolat

2014

Journal of Manufacturing Science and Engineering

371

287

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“…In just a few years this has changed significantly and in many laboratories today adult skin fibroblasts are routinely reprogrammed into pluripotent stem cells, which then give rise to a myriad of cell types. One of the most ingenious developments of this basic principle is the adaptation of commercial ink jet printers to produce growth factor arrays (by substituting the ink cartridges with a solution containing specific growth factors) in which cells are grown and induced to various levels of differentiation [5].…”

Section: Historical Backgroundmentioning

confidence: 99%

Are Brain Organoids Equivalent to Philosophical Zombies?

Bitar¹

2020

Preprint

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Along just over a century of research we moved from learning how to cultivate tissues in a dish to grasping the concepts for creating an entire brain in a vat. As we approach the divisive moment in which we can first detect signs of awareness in such artificially developed organoids, we need to lay foundation for what lays ahead. It is crucial that ethical, legal and moral implications of organoid research are clear and that boundaries are set to separate scientific progress from human life preservation. The largest obstacle may be the definition of consciousness itself, which has arguably been historically neglected by philosophy, psychology and neurosciences at large. One reason may be the difficulties posed by the underlying qualities of awareness, such as its subjective and heterogeneous nature. Another reason may lie on the possibly that consciousness is an overarching emergent property of our brain. For the time being, one can see brain organoids as philosophical zombies, physical analogues of the human brain which mimic sentient human reactions but lack experiential properties of sensation (a.k.a. qualia).

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“…It is a technology that has found its way in various industries outside the graphic art-printing field. Examples of applications include: biomedical and chemical sample handling [147][148][149], fuel mixing control [150,151], direct writing and packaging [152][153][154], optical component fabrication [155,156], micro fabrication [157,158], rapid prototyping [159][160][161] and manufacturing processes [162][163][164][165].…”

Section: Background On Inkjet Printing Technologiesmentioning

confidence: 99%

Development of collagen scaffold with internal channels via indirect rapid prototyping

Yeong¹

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In this project, a commercial 3-D inkjet printer, a representative Rapid Prototyping (RP) system, was employed to produce collagen scaffold using an indirect, sacrificial mould method. This method offers a great degree of design freedom compared to conventional as well as direct RP-fabrication method. To better understand the inkjet printing process, a physical model was established to relate the operating parameters to the dimensions of the part printed. The ability to control the parameters is important in order to achieve the predetermined morphology and resolution of the mould in this indirect fabrication technique. One major challenge in scaffold-based tissue engineering has been the limitation of cell migration and tissue ingrowth within the scaffolds. In this research, networks of channels were incorporated into the matrix as part of the architecture to overcome the limitation of nutrient diffusion. The shape, dimension, distribution, configuration as well as the orientation of the channels can be easily designed and controlled. The calculations of the channels lengths were performed based on the estimated flow shear in the channels and the oxygen mass balance required in the scaffold. An analytical model is presented to illustrate the enhancement of cell proliferation in scaffold with the internal channels. In vitro cell culture experiments with Human Primary Osteogenic Sarcoma (SaOS-2) cells were performed on control collagen scaffolds without internal channels and RPfabricated collagen scaffolds with internal channels. Cell Proliferation Assay studies showed significantly more cells were attached within the scaffolds with internal channels and these cells were distributed more homogenously. Scanning electron microscopy analysis and histological analysis confirmed that cells were attached in the interior region of the RP scaffolds. Higher cell penetration depth was also observed in the scaffolds through this improved technique. A customized direct perfusion bioreactor for the cultivation of cells on compliant scaffold was developed successfully. Significantly higher cell density was recorded in direct perfusion cultured scaffolds compared to the static cultured ones. The 1 ATTENTION: The Singapore Copyright Act applies to the use of this document. Nanyang Technological University Library combination of a RP-fabricated collagen scaffold and the direct perfusion bioreactor was able to maximize the proliferation of cells in scaffold and enhanced homogeneous distribution of cells. II

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Fabrication of Growth Factor Array Using an Inkjet Printer

Cited by 3 publications

References 45 publications

Bioprinting Technology: A Current State-of-the-Art Review

Bioprinting Technology: A Current State-of-the-Art Review

Are Brain Organoids Equivalent to Philosophical Zombies?

Development of collagen scaffold with internal channels via indirect rapid prototyping

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