Fabrication of microfluidic devices incorporating bead-based reaction and microarray-based detection system for enzymatic assay

Kim, Dae Nyun; Lee, Yeol; Koh, Won Gun

doi:10.1016/j.snb.2008.12.042

Cited by 44 publications

(22 citation statements)

References 34 publications

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“…By polymerizing solutions of PEG-DA and enzymes, also hydrogel microparticles can be formed. The mixture is therefore irradiated through a photomask, producing microparticles outside the system or directly in the microfluidic channels or chambers (Jang et al, 2012;Kim et al, 2009). …”

Section: Entrapment In Polymersmentioning

confidence: 99%

“…These are commercially available in a variety of shapes (spheres, beads, bars), sizes (in the nm and mm range) and materials (polymers, agarose, gold, glass) and can be purchased with surface groups like NH 2 ready for functionalization (Kim and Herr, 2013). As an alternative, enzymes can be incorporated into hydrogel microparticles produced in situ from precursor solutions like PEG-DA (Jang and Koh, 2010;Kim et al, 2009) which are mixed with the enzyme solution and polymerized using UV irradiation.…”

Section: Fabricationmentioning

confidence: 99%

“…One possibility is the definition of microfluidic structures including steps (Ito et al, 2011;Malecha et al, 2009) or micropillars (Jang and Koh, 2010;Kim et al, 2009) which stop the movement of the particles in the fluid stream at one point of the system. Wires incorporated into microfluidic channels can serve the same purpose (Blanes et al, 2007).…”

Section: Fabricationmentioning

confidence: 99%

“…Typical detection techniques employed in systems with microparticles are either amperometry (Godino et al, 2010;Hernandez et al, 2013;Ito et al, 2007Ito et al, , 2011Sheng et al, 2012) or optical techniques such as fluorescence measurements (Jang and Koh, 2010;Kim et al, 2009). The detection limits and linear ranges for these methods are in the same range as those reported in Section 3, making the immobilization strategies comparable in terms of measurement performance.…”

Section: Characteristics and Performancementioning

confidence: 99%

See 3 more Smart Citations

Microfluidic enzymatic biosensing systems: A review

Mross

Pierrat

Zimmermann

et al. 2015

Biosensors and Bioelectronics

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Section: Entrapment In Polymersmentioning

confidence: 99%

Section: Fabricationmentioning

confidence: 99%

Section: Fabricationmentioning

confidence: 99%

Section: Characteristics and Performancementioning

confidence: 99%

See 2 more Smart Citations

Microfluidic enzymatic biosensing systems: A review

Mross

Pierrat

Zimmermann

et al. 2015

Biosensors and Bioelectronics

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“…For example, a microfluidic device integrated with glass beads with enzymes was developed for the detection of glucose in the range of 1-10 mM. The hydrogen peroxide generated from the enzyme reaction between glucose in the sample and glucose oxidase immobilized on the surface of microbeads could be quantified by Amplex Red with fluorescent readout [129].…”

Section: Other Biomoleculesmentioning

confidence: 99%

Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays

Yue

Yang

2016

Microfluidic Methods for Molecular Biology

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Early screening and diagnosis of diseases require the development of sensitive, reliable, and inexpensive high-throughput assays. There is a growing need for innovative diagnostic technologies that provide accurate detection of a broad range of diseases and enhance laboratory productivity. Developments in micro-and nanotechnology have advanced the "lab-on-a-chip" concept towards a new generation of point-of-care diagnostic devices that enable parallel detection of multiple analytes in small-volume samples with high sensitivity in a short time. These features fulfill some of the important criteria of bioanalysis used for biochemical studies, environmental analyses, and clinical diagnostics. However, the multiplexing capability of microfluidic-based assay is limited compared to conventional flow cytometric assays, particularly for encoded microbead-based assays, which are capable of simultaneously analyzing multiple analyte. It is possible to incorporate the microbead-based assays into microfluidic devices in order to retain all the advantages of the microdevice and significantly improve multiplexing capability. In this chapter, we summarize the latest development in microbeadbased assays and discuss the integration of the microbead technology with microfluidic devices. Applications of the integrated approach in multiplexed protein and DNA analysis are growing rapidly in the areas of biomarker research, cancer screening, and disease diagnosis. The prospects for future development and commercialization of these microbead-based microfluidic devices are also discussed.

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Microfluidic Devices for Bioapplications

Yeo

Chang

Chan

et al. 2010

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Harnessing the ability to precisely and reproducibly actuate fluids and manipulate bioparticles such as DNA, cells, and molecules at the microscale, microfluidics is a powerful tool that is currently revolutionizing chemical and biological analysis by replicating laboratory bench-top technology on a miniature chip-scale device, thus allowing assays to be carried out at a fraction of the time and cost while affording portability and field-use capability. Emerging from a decade of research and development in microfluidic technology are a wide range of promising laboratory and consumer biotechnological applications from microscale genetic and proteomic analysis kits, cell culture and manipulation platforms, biosensors, and pathogen detection systems to point-of-care diagnostic devices, high-throughput combinatorial drug screening platforms, schemes for targeted drug delivery and advanced therapeutics, and novel biomaterials synthesis for tissue engineering. The developments associated with these technological advances along with their respective applications to date are reviewed from a broad perspective and possible future directions that could arise from the current state of the art are discussed.

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Fabrication of microfluidic devices incorporating bead-based reaction and microarray-based detection system for enzymatic assay

Cited by 44 publications

References 34 publications

Microfluidic enzymatic biosensing systems: A review

Microfluidic enzymatic biosensing systems: A review

Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays

Microfluidic Devices for Bioapplications

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