Fabrication of nano‐hydroxyapatite on electrospun silk fibroin nanofiber and their effects in osteoblastic behavior

Abstract

In this study, a novel tissue engineering scaffold material of electrospun silk fibroin/nano-hydroxyapatite (nHA) biocomposite was prepared by means of an effective calcium and phosphate (Ca-P) alternate soaking method. nHA was successfully produced on regenerated silk fibroin nanofiber as a substrate within several minutes without any pretreatments. The morphologies of both nonmineralized and mineralized nanofibers were analyzed using a field-emission scanning electron microscopy (FESEM). The crystallographic phases of the nHA were analyzed using X-ray diffraction (XRD). Fourier transform infrared (FTIR) spectrophotometer and thermogravimetry analyses (TGA) were employed to determine the type of functional groups and the amount of nHA presenting in the silk/nHA biocomposite nanofibers, respectively. The osteoblastic activities of this novel nanofibrous biocomposite scaffold were also investigated by employing osteoblastic-like MC3T3-E1 cell line. The cell functionality such as alkaline phosphatase (ALP) activity was ameliorated on mineralized nanofibers. All these results indicated that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering.

Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds

“…Thus, in order to use fibrous scaffolds when subject to tensional/compressional stresses in bone tissue engineering application, enhancement in scaffold mechanical properties is highly desired. [20][21][22] In order to increase the mechanical strength, fibrous scaffolds could be fabricated by depositing HAP on the surface or growing HAP within the interior of electrospun nanofibers. 22,23 Though various studies for the incorporation of osteoconductive HAP nanoparticles (nanohydroxyapatite, nHAP) in polymer fibers were reported, 24,25 there have been a relatively limited number of investigations for CS or SF nanofibers containing HAP nanoparticles.…”

“…[20][21][22] In order to increase the mechanical strength, fibrous scaffolds could be fabricated by depositing HAP on the surface or growing HAP within the interior of electrospun nanofibers. 22,23 Though various studies for the incorporation of osteoconductive HAP nanoparticles (nanohydroxyapatite, nHAP) in polymer fibers were reported, 24,25 there have been a relatively limited number of investigations for CS or SF nanofibers containing HAP nanoparticles. 26,27 A more extensive experimental scenario is essential to combine proliferation-differentiation factors with mechanical stability of the scaffold.…”

Enhanced mechanical properties and pre-tension effects of polyurethane (PU) nanofiber filaments prepared by electrospinning and dry twisting

“…As seen in Fig. 1, in general, electrospun PU nanofibers are deposited as a randomly oriented nanofiber web, forming a highly porous structure, which is held together by interfiber bonding and crossing [2][3][4]. The diameter and thickness of the fibers in the nanofiber mats obtained in this manner were 660±50 nm and~15 μm, respectively.…”

“…Nanofibers have recently attracted a great deal of attention because of their unique properties and promise for many applications, such as in electrical, biomedical, and protective products [1][2][3][4]. Several techniques, such as drawing [5], template synthesis [6,7], phase separation [8], selfassembly [9][10][11], and electrospinning [12,13], have been developed to generate nanofibers.…”

Silk fibroin/sodium alginate fibrous hydrogels regulated hydroxyapatite crystal growth

“…At the same time, the mechanical properties of composites had 40 MPa in bending strength and 2.5 GPa in Young's modulus. Wei et al studied HAp crystals depositing on regenerated silk fibroin (SF) nanofibers by a biomimetic Ca-P method [13]. The results exhibited HAp crystals with 30 nm diameter distributing on the surface of SF nanofibers.…”