Solid-state complementary metal oxide semiconductor (CMOS)-compatible glucose fuel cells, with carbon nano horn (CNH) films with different amounts of CNH (wt.%) and glucose solution were investigated. CNH content of 3 wt.% with 30 mM glucose solution was found the highest open circuit voltage (OCV) of 420 mV, a power density of 2.83 µW/cm2 and current density of 6.73 µA/cm2. This is the highest value compared with the conventional glucose fuel cell. The OCV and power density increased together with the fuel cell concentration. The developed fuel cell uses materials that are bio-compatible with the human body (CNH-glucose). As a result, OCV of 420 mV with a CNH content of 3 wt.% while improvements in the performance of the CMOS-compatible fuel cell were obtained, and the parameters affecting the performance of the fuel cell were identified. This glucose fuel cell was fabricated using CMOS semiconductor processes on a silicon wafer. This work presents a (5 mm × 5 mm) solid-state CMOS compatible glucose fuel cell that 420 mV of OCV. It is the highest value for a glucose fuel cell when the anode area is (4.2 mm × 4.6 mm). These findings are significant in realizing mobile or implantable devices that can be used for biomedical applications.