Facile and controllable one-step fabrication of molecularly imprinted polymer membrane by magnetic field directed self-assembly for electrochemical sensing of glutathione

Zhu, Wanying; Jiang, Guoyi; Xu, Lei; Li, Bingzhi; Cai, Qizhi; Jiang, Huijun; Zhou, Xuemin

doi:10.1016/j.aca.2015.05.036

Cited by 77 publications

(19 citation statements)

References 60 publications

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“…These applications have just risen from the recent development of imprinting strategies due to the complexity of imprinting macromolecules. This has to take in account many different interactions, simultaneously occurring around the template molecule, and the template insolubility into organic solvents generally used for the polymeric synthesis [26][27] . However, several MIPs have been developed for peptide enrichment and employed in the sample preparation of proteomic workflows [28][29][30][31][32][33][34][35] .…”

Section: Introducionmentioning

confidence: 99%

Exploring the peptide retention mechanism in molecularly imprinted polymers

et al. 2017

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Molecularly imprinted polymers (MIPs) have been used as useful sorbents in solid-phase extraction for a wide range of molecules and sample matrices. Their unique selectivity can be fine-tuned in the imprinting process and is crucial for the extraction of macromolecules from complex matrices such as serum. A relevant example of this is the application of MIPs to peptides in diagnostic assays. In this article the selectivity of MIPs, previously implemented in a quantitative mass-spectrometric assay for the biomarker pro-gastrin-releasing peptide, is investigated. Partial least squares regression was used to generate models for the evaluation and prediction of the retention mechanism of MIPs. A hypothesis on interactions of MIPs with the target peptide was verified by ad hoc experiments considering the relevant peptide physicochemical properties highlighted from the multivariate analysis. Novel insights into and knowledge of the driving forces responsible for the MIP selectivity have been obtained and can be directly used for further optimization of MIP imprinting strategies. Graphical Abstract Applied analytical strategy: the Solid Phase Extraction (SPE) of digested Bovin Serum Albumin (BSA), using Molecularly Imprinted Polymers (MIP), is followed by the liquid chromatography-mass spectrometry (LC-MS) analysis for the identification of the retained peptides. The further application of multivariate analysis allows setting up a Partial Least Square (PLS) model, which describes the peptide retention into the MIP and gives additional knowledge to be used in the optimization of the MIP and the whole SPE method.

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Section: Introducionmentioning

confidence: 99%

Exploring the peptide retention mechanism in molecularly imprinted polymers

et al. 2017

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“…An MMIP is a combination of magnetic particles with an MIP. Magnetic particles widely used in the synthesis of MMIPs are Ni [ 60 ], NiO [ 61 ], γ-Fe 2 O 3 [ 61 ], and Fe 3 O 4 [ 62 , 63 , 64 , 65 ]. The most commonly used magnetic particle is Fe 3 O 4 due to its low toxicity and easy fabrication [ 66 ].…”

Section: Methodsmentioning

confidence: 99%

Magnetic Molecularly Imprinted Polymers: An Update on Their Use in the Separation of Active Compounds from Natural Products

et al. 2022

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During the last few years, separation techniques using molecularly imprinted polymers (MIPs) have been developed, making breakthroughs using magnetic properties. Compared to conventional MIPs, magnetic molecularly imprinted polymers (MMIPs) have advantages in sample pretreatment due to their high specificity and selectivity towards analytes as a result of their larger specific surface areas and highly accessible specific binding sites. The techniques of isolation of active compounds from natural products usually require very long process times and low compound yields. When MMIPs are used in sample separation as Solid Phase Extraction (SPE) sorbents, the MMIPs are introduced into the dissolved sample and spread evenly, and they form bonds between the analyte and the MMIPs, which are then separated from the sample matrix using an external magnetic field. This process of separating analytes from the sample matrix makes the separation technique with MMIPs very simple and easy. This review discusses how to synthesize MMIPs, which factors must be considered in their synthesis, and their application in the separation of active compounds from natural products. MMIPs with magnetic core-shells made by co-precipitation can be a good choice for further development due to the high synthesis yield. Further optimization of the factors affecting the size and distribution of magnetic core-shell particles can obtain higher synthesis yields of MMIPs with higher adsorption capacity and selectivity. Thus, they can isolate target compounds from natural plants in high yields and purity.

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“…At the same time, because this weak effect itself is closer to the natural molecular recognition system, it has better applicability in biology, medicine, and other fields. [ 120 ] Nevertheless, this method also has certain shortcomings: the function of the functional monomer and the template molecule is a noncovalent bond, which makes the mechanism of the imprinting process not clear enough. At the same time, the change of the conditions during the preparation has a greater impact, which may lead to relatively poor selectivity.…”

Section: Synthesis Methods Of Protein Smipsmentioning

confidence: 99%

Nanomaterials‐Based Surface Protein Imprinted Polymers: Synthesis and Medical Applications

Pan

Hong

Xie

et al. 2020

Macro Chemistry & Physics

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Proteins are an essential part of organisms and play a very important role in life activities. Molecular imprinting technology (MIT) can prepare functional materials with specific identification abilities. In recent years, various molecularly imprinted materials have been widely used in the fields of solid‐phase extraction, bionic sensing, and on‐line analysis. Due to the limit of the properties of protein macromolecules, the development of MIT for protein molecules is relatively slow. With the continuous deepening on the research of nanomaterials and surface molecular imprinting technology, a large numbers of interesting and successful strategies appear on imprinted protein. Some surface protein imprinting materials with excellent properties and unique functions are applied in proteomics, biological imaging, medical diagnosis, and other fields, showing great potential application value. This paper summarizes the latest preparation and analysis strategies of surface protein imprinting based on nanomaterials, introduces medical application of protein imprinting materials in sample separation and purification, proteomics, biomedical and other aspects in recent years, and looks forward to the development opportunities and challenges in protein imprinting field.

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Facile and controllable one-step fabrication of molecularly imprinted polymer membrane by magnetic field directed self-assembly for electrochemical sensing of glutathione

Cited by 77 publications

References 60 publications

Exploring the peptide retention mechanism in molecularly imprinted polymers

Exploring the peptide retention mechanism in molecularly imprinted polymers

Magnetic Molecularly Imprinted Polymers: An Update on Their Use in the Separation of Active Compounds from Natural Products

Nanomaterials‐Based Surface Protein Imprinted Polymers: Synthesis and Medical Applications

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