Facile Fluorescence Dopamine Detection Strategy Based on Acid Phosphatase (ACP) Enzymatic Oxidation Dopamine to Polydopamine

Abstract

Facile and effective detection of dopamine (DA) plays a significant role in current clinical applications. Substantially, special optical nanomaterials are important for fabricating easy-to-control, cheap, selective, and portable fluorescence DA sensors with superior performance. Herein, carbon dots (CDs) prepared from melting method were applied as signal to establish a simple but effective fluorescence strategy for DA determination based on the enzymatic activity of acid phosphatase (ACP), which induces DA to form polydopamine (pDA). The formed pDA caused by the enzymatic oxidization of ACP toward DA can interact with CDs through the inner filter effect. Such behavior effectively quenched the CDs' fluorescence. The degree of fluorescence quenching of CDs was positively correlated with the DA content. Under the optimized reaction conditions, the proposed fluorescence method exhibited a comparable analytical performance with other DA sensors with good selectivity. Furthermore, this method has been successfully applied to detect DA in DA hydrochloride injection and human serum samples. It shows that this method features potential practical application value and is expected to be used in clinical research.

Self-Catalyzed Surface Reaction-Induced Fluorescence Resonance Energy Transfer on Cysteine-Stabilized MnO2 Quantum Dots for Selective Detection of Dopamine

“…These results indicated that the Cys-MnO 2 QD-based nanoplatform is reliable for the detection of DA. 8), Ca 2+ (9), Glu (10), Tyr (11), His (12), Ala (13), GSH (14), AA (15), Pro (16), and glucose (17). The concentration of DA is 50 μM, and the concentration of others is 500 μM.…”

“…Various techniques have been developed for the sensitive detection of DA, including surface plasmon resonance, colorimetric, , fluorescence, , chemiluminescence, , and electrochemical methods. , Among them, the fluorescence method shows the advantages of low cost, simple operation, and high sensitivity, which has been developed for quantitative detection of DA. ,, Furthermore, compared with electrochemical monitoring of DA, the fluorescence method is more convenient and feasible for it needs no additional electrode to the living body. It is an interesting strategy to modulate the fluorescence signal of the probe via controlling the polymerization of DA to form polydopamine (PDA). , However, many sensing strategies possess the disadvantages such as high cost for the preparation of the fluorescence probes, unclear detection mechanism, and low detection selectivity or sensitivity. To this end, it is significant to develop simple and convenient fluorescent probes with good sensitivity and selectivity for the detection of DA in real samples via a definite detection mechanism, which is helpful for clinical diagnosis and can provide a reference for in situ monitoring of DA …”

“…It is an interesting strategy to modulate the fluorescence signal of the probe via controlling the polymerization of DA to form polydopamine (PDA). 13,14 However, many sensing strategies possess the disadvantages such as high cost for the preparation of the fluorescence probes, unclear detection mechanism, and low detection selectivity or sensitivity. To this end, it is significant to develop simple and convenient fluorescent probes with good sensitivity and selectivity for the detection of DA in real samples via a definite detection mechanism, which is helpful for clinical diagnosis and can provide a reference for in situ monitoring of DA.…”

“…Figure 7. Selectivity of the Cys-MnO 2 QDs toward DA; the numbers represent the detection of DA (1), Fe 3+ (2), Fe 3+ + 2.5 mM oxalic acid (3), Fe 2+ (4), Zn 2+ (5), K + (6), Mg 2+ (7), Na + (8), NH 4 + (9), Ca 2+ (10), Glu(11), Tyr(12), His (13), Ala(14), GSH(15), AA(16), Pro(17), and glucose(18). (B) Anti-interference study of DA detection.…”

“…(B) Anti-interference study of DA detection. These numbers are represented as follows: Cys-MnO 2 QDs + DA (1), Cys-MnO 2 QDs + DA toward Fe 3+ + oxalic acid (2), Fe 2+ (3), Zn 2+ (4), K + (5), Mg 2+ (6), Na + (7), NH 4 + (8), Ca 2+ (9), Glu(10), Tyr(11), His(12), Ala (13), GSH(14), AA(15), Pro(16), and glucose(17). The concentration of DA is 50 μM, and the concentration of others is 500 μM.…”