Facile method to detect oligonucleotides with functionalized polydiacetylene vesicles

Utilization of chromic polydiacetylene assemblies as a platform to probe specific binding between drug and RNA

“…51,73 It is worthwhile to note that a phospholipid (dimyristoylphosphatidycholine, DMPC) is normally used for improving sensitivity of PDA assemblies to biomolecules in previous studies. 53,60–61 We demonstrate in this study that alcohol is an interesting additive for enhancing the sensitivity of PDA to biomolecules. The alcohol is also much cheaper compared to the DMPC.…”

Colorimetric detection of clinical DNA samples using an intercalator-conjugated polydiacetylene sensor

“…Few studies have detected nucleic acids by exploiting the colorimetric properties of PDA. One group described a colorimetric method for Contents lists available at ScienceDirect journal homepage: www.elsevier.com/locate/bios detecting oligonucleotides that involved hybridization with probe oligonucleotide-functionalized PDA vesicles (Wang et al, 2006;Wang and Ma, 2005). However, synthetic oligonucleotides-and not clinical targets-were detected in this study.…”

“…With regards to portable and more efficient analytic sensors for detecting dsDNA in clinical samples, conjugated polymer-based sensors (Ho et al, 2002(Ho et al, , 2005Jung et al, 2008Jung et al, , 2010Lee et al, 2007;Wang et al, 2006;Wang and Ma, 2005) have the important advantage of amplifying signals in response to external stimuli as compared to conventional sensors that are based on small molecules (Ali and Li, 2009;Du and Tang, 2011;Li et al, 2009;Li and Rothberg, 2004). The versatile and stable conjugated polydiacetylene (PDA) has useful structural and sensing properties when its head group is linked to ligands that can recognize external stressors such as pH, organic solvents, mechanical stress, and specific molecules.…”

Simple and highly sensitive detection of hepatotoxin microcystin-LR via colorimetric variation based on polydiacetylene vesicles

“…PDAs are non-fluorescent in the "blue-phase" but have fluorescence in the "red-phase" [26]. To date, the colorchangeable and fluorogenic properties of the stress-sensitive PDAs have already been employed for sensor development to detect virus [17,18], E. coli [27], nucleic acids [28,29], toxin peptide [20], ion [30,31,22], cyclodextrin [32,33], etc. In these cases, it is the shape changes of molecules during recognition events that trigger the sensor response.…”