Facile, rapid and efficient biofabrication of gold nanoparticles decorated with functional proteins

Abstract

We report a one-pot biological approach to fabricate gold nanoparticle (AuNP)-ZZ domain conjugates using peptide-functionalized proteins that can simultaneously direct both biomineralization and surface modification of AuNPs. In addition, immuno-AuNPs are readily prepared through the specific binding of antibodies to the ZZ domain on the AuNPs.

Protein-Functionalized Gold Nanoparticles for Antibody Detection Using the Darkfield Microscopic Observation of Nanoparticle Aggregation

“…AuNPs were synthesized in the presence of rBSA or BSA according to the method described for AuNP synthesis using the A3 peptide 15 . A solution consisting of AuCl4 -(0.25 mM), rBSA or BSA (2 μM), and HEPES buffer (10 mM, pH 8) was incubated overnight at 27°C.…”

“…To overcome the limitations of the above-mentioned methods, the synthesis of AuNP by using peptides and proteins has been reported, wherein the reducing ability of amino acid residues such as cysteine and tyrosine is utilized 12,13 . For example, AuNPs with a size of 10-15 nm were synthesized by an A3 peptide (AYSSGAPPMPPF) using tyrosine residues as the reducing agents 14,15 . Slocik et al also reported AuNP synthesis using YYY peptide 16 .…”

A Comprehensive Extraction Study Using a Mono-alkylated Diglycolamic Acid Extractant: Comparison Between a Secondary Amide Group and a Tertiary Amide Group

“…The pH of the aqueous solutions was adjusted by the addition of either HNO 3 or NaOH to a 10 mM or 100 mM 2-morpholinoethanesulfonic acid (MES) buffer. In the extraction tests with metal chlorides (Ru(III), Rh(III), Pd(II), Hf(IV), Os(IV), Ir(III), Pt(IV), and Au(III)), the pH was adjusted by the addition of HCl to a 50 mM sodium acetate buffer at a constant ionic strength of 0.1 M NaCl because the MES buffer can easily reduce Au(III) to Au(0) [23][24][25]. In the extraction of K(I), Hg(II), and Ag(I), the contamination of aqueous solutions by potassium ions and chloride ions from the pH electrode was prevented.…”

Comprehensive Extraction Study Using N,N-Dioctyldiglycolamic Acid

“…(Caution: do not use the MES buffer for Au(III) extraction because MES can easily reduce Au(III) to Au(0). [19][20][21] ) In extraction tests of rare-earth metal ions, the pH was adjusted by dilution with HNO3 without a buffer. For Ag(I) and Hg(II) extraction, the contamination of aqueous solutions by chloride ions from the pH electrode was prevented.…”