Factors affecting conjugative transfer rates of plasmids in batch and continuous cultures

Yao, Pei-Xin; Ohtake, Hisao; Toko, Kiyoshi

doi:10.1016/0922-338x(90)90215-i

Cited by 5 publications

(1 citation statement)

References 15 publications

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“…The GEMS should be nonconj ugative so that the recombinant plasmid may not be transferred to indigenous microbes after being released deliberately or accidentally into a natural environment. We have been studying the physical and chemical effects on rate of plasmid transfer between donor and recipient cells in a microcosm20, 22) to predict the fate of plasmidbearing microorganisms released in a natural environment. In laboratory experiments, plasmid transmissbility is usually tested at its optimum condition.…”

Section: Introductionmentioning

confidence: 99%

Effect of solid particles on conjugal transfer of plasmid R100-1 in Escherichia coli.

Asakura

Toko

1993

Bulletin of Japanese Society of Microbial Ecology

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Effect of solid particles on transfer of plasmid R100-1 from Escherichia coli LC102 to a rifampicin-resistant mutant of E. coli DHl was investigated using nutritionally-rich (L medium) and-poor medium (supernatant of a mixed liquor). Plasmid transfer was most significantly inhibited in the presence of bentonite. The addition of 2g.1-1 bentonite decreased the specific rate of transconjugant increase in mating to one ninetieth in the former medium, and one twenty-third in the latter. Activated sludge decreased the rates to one third and one fifth, respectively, whereas sterilized activated sludge did not affect the rates.

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Section: Introductionmentioning

confidence: 99%

Effect of solid particles on conjugal transfer of plasmid R100-1 in Escherichia coli.

Asakura

Toko

1993

Bulletin of Japanese Society of Microbial Ecology

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Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor

Boquien

Corrieu

1992

Appl Microbiol Biotechnol

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The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythromycin or chloramphenicol resistance): pIL205 (self-transmissible), pIL252, pIL253 (non-transmissible but mobilizable by pIL205, respectively small and large copy number) and pE194 (inserted in the chromosome). Only self-transmissible plasmid pIL205 was transferred, with frequencies ranging from 10(-7) to 10(-8) after 12 h of fermentation. These frequencies were 60-400 times lower than in unstirred M17 broth and 100,000 times lower than on agar medium. In the latter case, non-transmissible plasmids pIL252 and pIL253 were mobilized by pIL205 with a frequency of about 10(-5) - 10(-6).

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Physiological similarity and bioreactor scale-up

Votruba

Sobotka

1992

Folia Microbiol

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Factors affecting conjugative transfer rates of plasmids in batch and continuous cultures

Cited by 5 publications

References 15 publications

Effect of solid particles on conjugal transfer of plasmid R100-1 in Escherichia coli.

Effect of solid particles on conjugal transfer of plasmid R100-1 in Escherichia coli.

Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor

Physiological similarity and bioreactor scale-up

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