Factors affecting in vitro adventitious shoot formation on internode explants of Citrus aurantium L. cv. Brazilian

Marques, Natália; Nolasco, Gustavo; Leitão, José

doi:10.1016/j.scienta.2011.03.018

Cited by 13 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Considering citrus, epicotyl-derived explants are the most frequently used for genetic engineering in different cultivars (ALMEIDA et al, 2002;SILVA et al, 2005;GARCÍA-LUIS et al, 2006;NWE et al, 2014). Other explants, such as leaf discs-(KHAN et al, 2009), hypocotyl-(MAGGON; SINGH, 1995), cotyledon-(TAVANO et al, 2009), and internodal- (MARQUES et al, 2011) derived segments have also been tested; however, they presented lower efficiency. In this study, the adventitious shoot development occurred more frequently from epicotyl-derived explants, with more than 50% of responsive explants and more than four shoots developed per responsive explant ( Table 1), regardless of the cultivar.…”

Section: Resultsmentioning

confidence: 99%

“…In vitro citrus organogenesis is usually induced from juvenile tissue, including epicotyl-derived segments extracted from in vitro germinated seedlings (GARCIA-LUIS et al, 2006;SORIANO et al, 2012) or internodalderived segments extracted from juvenile plants cultivated under greenhouse conditions (TAVANO et al, 2009;MARQUES et al, 2011). In most of citrus tissue culture protocols, the development of adventitious shoots occurred in culture media supplemented with growth regulators (SILVA et al, 2005;OLIVEIRA et al, 2010;BASSAN et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro organogenesis and genetic transformation of mandarin cultivars

et al. 2019

View full text Add to dashboard Cite

The in vitro organogenesis of Fremont (Citrus clementina x ), Citrus reticulataThomas (Citrus reticulata), and Nules (Citrus clementina) mandarins was evaluated aiming to optimize a regeneration protocol that could be applied in genetic transformation. The use of epicotyl-derived explants resulted in higher explant responsiveness and number of shoots developed per explant when compared with the use of internodal-derived explants. The highest efficiency in shoot regeneration was observed in the presence of 1 mg L-1 of BAP, regardless of the explant type and cultivar. The in vitro organogenesis protocol produced transgenic plants from three mandarin cultivars expressing attA gene under the control of phloem-specific promoters.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro organogenesis and genetic transformation of mandarin cultivars

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Maximum number of shoots per explant was observed in Volkameriana at 1 ppm of BA (Table 2). Different researchers (Begum et al, 2008;Marques et al, 2011) observed in Citrus aurantium (sour orange) that 1 ppm of BA is the optimum concentration for maximum number of shoots per explant. Citrange 'Carrizo' and Poncirus trifoliata 'Serra' showed highest number of shoots per explant at 2ppm of BA (Table 2).…”

Section: Proliferation Phasementioning

confidence: 98%

“…Rootstocks Citrange 'Carrizo' and Volkameriana also presented 100% shoot induction in 1 ppm of BA (Table 2). Many studies (Marques et al, 2011;Sharma et al, 2009;Pena et al, 1995;Rani et al, 2004) reported in various Citrus species highest proliferation percentage in that 1 ppm of BA. Also, it was observed that by increasing the BA concentration from 2 to 4 ppm, proliferation percentage was decreased in most of the rootstocks studied.…”

Section: Proliferation Phasementioning

confidence: 99%

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

Salis

Papadakis

Kintzios

et al. 2017

Not Bot Horti Agrobo

View full text Add to dashboard Cite

The behavior of six citrus rootstocks, Volkameriana, Citrumelo 'Swingle', Citrange 'Carrizo', Poncirus trifoliata 'Serra', Poncirus trifoliata 'Rubidoux' and Poncirus trifoliata 'Flying Dragon', in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock 'Swingle' showed a higher number of roots per explant (4.2) followed by 'Flying Dragon' (3.93) and 'Carrizo' (3.23) rootstocks. The rootstocks 'Swingle' (140.8 mm), Volkameriana (148 mm) and 'Flying Dragon' (131.12 mm) had significantly higher root length per explant compared to 'Carrizo' (31 mm) and 'Rubidoux' (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata 'Serra' and Citrumelo 'Swingle') and 0.60 (Volkameriana and Citrumelo 'Swingle'). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

show abstract

“…Genetic transformation of Citrus is a promising tool that enables the introduction of desirable traits without altering the genetic background [25]. Genetic transformation of citrus has been reported, by using several methods (Table 1).…”

Section: Transformation Studies In Citrusmentioning

confidence: 99%

Genetic Transformation in Citrus

Dönmez

Şimşek

İzgü

et al. 2013

The Scientific World Journal

View full text Add to dashboard Cite

Citrus is one of the world's important fruit crops. Recently, citrus molecular genetics and biotechnology work have been accelerated in the world. Genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. Citrus transformation has now been achieved in a number of laboratories by various methods. Agrobacterium tumefaciens is used mainly in citrus transformation studies. Particle bombardment, electroporation, A. rhizogenes, and a new method called RNA interference are used in citrus transformation studies in addition to A. tumefaciens. In this review, we illustrate how different gene transformation methods can be employed in different citrus species.

show abstract

Factors affecting in vitro adventitious shoot formation on internode explants of Citrus aurantium L. cv. Brazilian

Cited by 13 publications

References 34 publications

In vitro organogenesis and genetic transformation of mandarin cultivars

In vitro organogenesis and genetic transformation of mandarin cultivars

In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

Genetic Transformation in Citrus

Contact Info

Product

Resources

About