Factors affecting plasma progesterone concentration and the retrospective determination of time of ovulation in cyclic mares

Nagy, P.; Huszenicza, G.; Reiczigel, Jenő; Juhász, Judit; Kulcsár, Margit; Abaváry, K; Guillaume, Daniel

doi:10.1016/s0093-691x(03)00211-5

Cited by 22 publications

(17 citation statements)

References 17 publications

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“…Progesterone profiles observed in the control group were consistent with those described by Nagy et al [24]. Hormone levels were affected by the infusion, as there was a treatment × day interaction ( P < 0.01) and a tendency to lower progesterone levels in the infused group on day 2 ( P = 0.07).…”

Section: Discussionsupporting

confidence: 89%

Effect of a povidone-iodine intrauterine infusion on progesterone levels and endometrial steroid receptor expression in mares

Kalpokas¹,

Perdigón²,

Rivero

et al. 2010

Acta Vet Scand

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BackgroundIntrauterine infusions have been widely used for the treatment of endometritis in the mare. Nevertheless, their consequences on endocrine and endometrial molecular aspects are unknown. We studied the effect of a 1% povidone-iodine solution intrauterine infusion on progesterone levels, endometrial histology and estrogen (ERα) and progesterone (PR) receptor distribution by immunohistochemistry.MethodsFourteen healthy mares were used in this study. Estruses were synchronized and seven mares were treated with intrauterine infusions at days 0 and 2 post ovulation of two consecutive estrous cycles. Uterine biopsy samples were taken on days 6 and 15 post ovulation.ResultsThe treatment did not induce an inflammatory response indicating endometritis, neither affected the ERα. However, it reduced the percentage of PR positive cells (PPC) on day 6 (deep glandular epithelium, control: 95.7 vs. infused: 61.5, P < 0.05). Treated mares tended to have lower progesterone levels on day 2 (3.9 ng/ml vs. 6.6 ng/ml, P = 0.07), and higher levels on day 15 compared with controls (4.4 ng/ml vs. 1.3 ng/ml, P = 0.07).Conclusiona 1% povidone-iodine infusion during days 0 and 2 post ovulation in healthy mares did not induce histological changes indicating endometritis, but altered progesterone concentrations and reduced the expression of endometrial PR at day 6 without affecting the ERα. These changes could reduce embryo survival.

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Section: Discussionsupporting

confidence: 89%

Effect of a povidone-iodine intrauterine infusion on progesterone levels and endometrial steroid receptor expression in mares

Kalpokas¹,

Perdigón²,

Rivero

et al. 2010

Acta Vet Scand

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“…To prove that even little differences in P4 levels (although the levels of P4 are > 10 ng/ml) in pregnant and nonpregnant animals are the cause of conception failure, we probably need specifically designed studies. The luteolytic process in a regular cycle of nonpregnant mares takes place around day 16 post ovulation (Nagy et al, 2004), which was, according to the decrease in size, also apparent in our study.…”

Section: Discussionsupporting

confidence: 83%

The luteal blood flow, area and pixel intensity of corpus luteum, levels of progesterone in pregnant and nonpregnant mares in the period of 16 days after ovulation

Šichtař¹,

Rajmon²,

Hošková³

et al. 2013

Czech J. Anim. Sci.

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ABSTRACT:The objective of the present study was to examine if luteal blood flow (LBF) monitoring could be used as an additional prognostic tool for early pregnancy diagnosis, and we particularly focused on the differences in LBF between pregnant and nonpregnant mares. Furthermore, other possible developmental differences of corpus luteum (CL) between pregnant and nonpregnant mares were evaluated. The CL (n = 119) of 27 mares were monitored once daily in B-and Power-Doppler Mode on days 1, 2, 9, 12, and 16 after ovulation (day 0 = ovulation). The data were evaluated using the MIXED Linear Model with repeated measures, and parameters were estimated by the REML method. The course of LBF, area of CL, and pixel intensity differed in nonpregnant mares on a day-to-day basis in contrast to more stable values in pregnant mares. Further, the profiles of the courses were identical until day 9, but since day 12 the differences between pregnant and nonpregnant mares started to be prominent. The LBF, pixel intensity, and level of progesterone (P4) were similar in all mares until day 16, when smaller LBF, lower pixel intensity, and lower levels of P4 were found in nonpregnant mares (P = 0.04, P = 0.02, P < 0.05, respectively). In pregnant and nonpregnant mares the LBF was weakly (r = 0.29 in both) and pixel intensity strongly (r = 0.48 and 0.59, respectively) correlated to the levels of P4. LBF was strongly correlated to the area of CL in pregnant as well as nonpregnant mares (r = 0.72 and 0.64, respectively). In accordance with the results presented in our study we can state that LBF monitoring is not a suitable tool for early pregnancy diagnosis or prognosis as the differences between pregnant and nonpregnant mares are notable -similarly to other indicators of CL status -just after the onset of luteolysis (day 16) when embryo itself is detectable.

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“…Pierson e Ginther (1985) citam que o tamanho e a ecogenicidade luteal podem refletir na produção de P4. Entretanto, Townson et al (1989), Ferreira (2000, Nagy et al (2004) e Castro (2005 verificaram que o tamanho do CL parece não ter efeito significativo sobre a concentração plasmática de P4. No presente estudo não foi realizada tal avaliação.…”

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“…No grupo das receptoras subférteis três éguas apresentaram baixas concentrações de P4 baixa (0,1; 1,20 e 1,01ng/ mL) sendo que as duas últimas estavam no segundo dia pós-ovulação. Normalmente nesta fase o CL ainda apresenta baixa síntese de P4 (Aupperle et al 2000, Arruda et al 2001, Nagy et al 2004. As demais éguas apresentaram concentrações acima de 2,0ng/mL, o que segundo Ball & Daels (1997) têm sido sugeridas como suficientes para a manutenção da gestação.Embora a P4 elevada na fase estrogênica e baixa na fase progesterônica possam determinar respectivamente falhas na ovulação ou ambiente uterino inadequado para a manutenção de gestação (Castro 2005), sendo causas de infertilidade em éguas, neste experimento tais fatos não foram observados.…”

Section: Discus Discus Discus Discus Discussão São São São Sãounclassified

Estudo comparativo de éguas repetidoras ou não de cio através da avaliação histológica do endométrio e das concentrações plasmáticas de progesterona

Eigenheer-Moreira

Fernandes²,

Queiroz³

et al. 2007

Pesq. Vet. Bras.

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Comparative study of repeat breeds and healthy mares through endometrial Comparative study of repeat breeds and healthy mares through endometrial Comparative study of repeat breeds and healthy mares through endometrial Comparative study of repeat breeds and healthy mares through endometrial Comparative study of repeat breeds and healthy mares through endometrial histology and plasmatic progesterone concentrations histology and plasmatic progesterone concentrations histology and plasmatic progesterone concentrations histology and plasmatic progesterone concentrations histology and plasmatic progesterone concentrations.] Estudo comparativo de éguas repetidoras ou não de cio através da avaliação histológica do endométrio e das concentrações plasmáticas de progesterona. Pesquisa Veterinária Brasileira 27 (12) The study aimed to compare endometrial histology and plasmatic progesterone (P4) concentration of repeat breeds and healthy mares. The hypothesis was that there is a correlation between infertility and endometrial histology and P4 concentration in both groups. A total of 36 Campolina and Mangalarga Marchador mares in reproductive age (3-23 years) were used, 11 of them were healthy mares (Control group, 7 embryo recipient and 4 embryo donors), and 25 repeat breeders (10 embryo recipient and 15 embryo donors), classified as based on their reproductive history. Endometrial and blood samples were collected for respectively histological and plasma progesterone concentration evaluation. The endometrial samples obtained after biopsy were fixed in Bouin's fluid, processed, included in paraffin, and stained with HematoxylinEosin (HE) for histopathological examination. Plasmatic progesterone concentrations were evaluated by enzyme immunoessay (ELISA). There was no correlation between progesterone concentration and fertility. But there was a positive correlation between age and fertility, as older mares had major tendency of subfertility than younger ones. There was also a correlation between biopsy categories and fertility, as more histological alterations were found, higher were the chances for the mares to be subfertile. However not all mares classified as Category I and II maintained pregnancy until parturition. Other factors could influence pregnancy maintenance. In the same way, not all mares in Category III were infertile. The endometrial biopsy was shown to be an easy and cheap diagnostic technique with minimal discomfort to the animals and, together with other data, to be a very important component in the investigation of mare fertility.

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Factors affecting plasma progesterone concentration and the retrospective determination of time of ovulation in cyclic mares

Cited by 22 publications

References 17 publications

Effect of a povidone-iodine intrauterine infusion on progesterone levels and endometrial steroid receptor expression in mares

Effect of a povidone-iodine intrauterine infusion on progesterone levels and endometrial steroid receptor expression in mares

The luteal blood flow, area and pixel intensity of corpus luteum, levels of progesterone in pregnant and nonpregnant mares in the period of 16 days after ovulation

Estudo comparativo de éguas repetidoras ou não de cio através da avaliação histológica do endométrio e das concentrações plasmáticas de progesterona

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