Factors affecting serotonin uptake into human platelets

Codd, Ellen E.; McAllister, Thomas W.; Walker, Richard F.

doi:10.1007/bf00174506

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…Gender effects are small with females having approximately 10% higher whole blood 5-HT levels than males (Cook et al 1988). AfricanAmerican subjects have been reported to have higher whole-blood 5-HT levels in preliminary studies (Cuccaro et al 1993;Cook et al 1995).Seasonal variations have been reported for some parameters related to 5-HT metabolism and function in humans, including both platelet 5-HT uptake (Arora et al 1984;Codd et al 1988;Egrise et al 1986;Marazziti et al 1990), content (Brewerton et al 1993Jakovljevic et al 1997 pramine binding (Egrise et al 1986;DeMet et al 1991). However, the results on circannual changes in platelet 5-HT content have been inconsistent (Brewerton et al 1993;Jakovljevic et al 1997; Wirz-Justice and Puhringer 1978;Mann et al 1992).…”

mentioning

confidence: 99%

Serotonin Transporter and Seasonal Variation in Blood Serotonin in Families with Obsessive-Compulsive Disorder

Hanna

Himle

Curtis

et al. 1998

Neuropsychopharmacology

142

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The serotonin transporter (HTT) is a candidate gene for obsessive-compulsive disorder (OCD) that has been associated with anxiety-related traits. The long (1) and short (s) variants of theThe serotonin transporter (HTT = SLC6A4) is a plausible candidate gene for obsessive-compulsive disorder (OCD) and several other psychiatric disorders (Altemus et al. 1996;Lesch et al. 1995;Ogilvie et al. 1996). It has been associated with anxiety-related traits in two predominantly male samples (Gelernter et al. 1995;Ramamoorthy et al. 1993). A complementary deoxyribose nucleic acid (cDNA) encoding the 5-HTT in the human midbrain raphe complex has been isolated, and its identity with the human platelet serotonin (5-HT) transporter site has been demonstrated (Lesch et al. 1993a,b).HTT gene transcription is regulated by a deletion/ insertion polymorphism in the promoter (Heils et al. 1995). The long (I) and short (s) variants of this polymorphic region have different transcriptional efficiencies in transfection assays and lymphoblastoid cell lines Lesch et al. 1996). The short allele reduces the transcriptional efficiency of the HTT gene promoter, resulting in decreased 5-HTT binding and 5-HT uptake in lymphoblasts . Studies of HIT gene expression in lymphoblast cell lines from 10 subjects with different genotypes suggested that the polymorphism has more of a dominant-recessive than a codominant-additive effect, with the short variant ap-0893-133X/98/$19.00 PII S0893-133X(97)00097-3 NEUROPSYCHOPHARMACOLOGY 1998-VOL. 18, NO. 2 pearing dominant to the long variant. However, the dominant mode of action of the short allele has been questioned (Goldman 1996).Because of the small sample size in that study and the importance of the HTT, we examined the relationship between the HTT promoter variant and whole blood 5-HT concentration in 70 individuals from 20 families ascertained through children and adolescents between 10 and 17 years of age with a DSM-III-R diagnosis of OCD. Sampling through early-onset cases can be a useful strategy in identifying a more clearly genetic or homogeneous form of a disorder (Lander and Schork 1994). Family studies have demonstrated that OCD is often familial and that the morbid risk of the disorder is higher among the relatives of early-onset probands than among the relatives of late-onset probands (Bellodi et al. 1992;Pauls et al. 1995).The selective response of OCD to potent 5-HT transporter inhibitors (serotonin reuptake inhibitors, SRis) has suggested that a serotonergic abnormality may be involved in OCD (Goodman et al. 1990;Leonard et al. 1989). The 5-HTT is an initial site of action for those drugs and is crucial to the termination of serotonergic neurotransmission (Amara and Kuhar 1993). Two treatment studies of childhood OCD using clomipramine, a potent 5-HT transporter inhibitor, have demonstrated a significant correlation between clinical improvement and a marked reduction in either platelet 5-HT or whole blood 5-HT content, reflecting the effect of the drug on the platelet uptake site (F...

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confidence: 99%

Serotonin Transporter and Seasonal Variation in Blood Serotonin in Families with Obsessive-Compulsive Disorder

Hanna

Himle

Curtis

et al. 1998

Neuropsychopharmacology

142

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“…Furthermore, there was a significant difference in age between atopic (35+11 yr) and nonatopic patients (47+ 12 yr). This could explain a higher uptake inhibition for older patients as has previously been described [13]. Finally, it is noteworthy that in the Malmgren et al [7] study there was an aggravation of this inhibition when PRP was diluted in PPP o^ the same subject which, among other serum factors, contained IgE.…”

Section: Discussionmentioning

confidence: 54%

“…It is unlikely that the MIBG uptake we measured after incubation without IgE was dependent on factors known to alter serotonin uptake for the following reasons; the experiments were performed al the same time of day to remove the effect of time on serotonin uptake [13]. The normal subjects we studied belonged to the same age group and were also free of psychiatric illness, both factors altering serotonin uptake [13,14]. Consequently, our results before incubation with IgE are comparable to those described in previous studies [9.10].…”

Section: Discussionmentioning

confidence: 99%

The inhibitory effect of immunoglobulin E on monoamine uptake in human platelets

Lara¹,

Vuillemin

Rondoyanni

et al. 1991

Clin Experimental Allergy

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The uptake of the monoamine radiolabelled tracer 125I-meta-iodo-benzyl-guanidine (MIBG) was studied in vitro in platelets from 15 healthy volunteers and five allergic patients whose serum IgE concentration was 37 +/- 33 and 650 +/- 200 IU/ml, respectively. The MIBG uptake was determined by means of a gamma counter after incubation in a buffer. Binding of IgE to platelets significantly reduced MIBG uptake both in healthy subjects and in allergic patients. This reduction was significantly related to the action of IgE as it did not occur after binding of IgG. Moreover, MIBG uptake plotted versus increasing concentration of IgE (ranging from 10 to 3000 IU/ml) was fitted by a classical sigmoidal curve which was not significantly different between healthy subjects and allergic patients. Lastly, the effect of IgE on MIBG uptake was due to the binding of the sole IgE as it was not modified by subsequent addition of anti-IgE. It is concluded that the binding of the sole IgE to its specific receptor on platelets alters the transport of monoamines.

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“…Numerous studies have reported on variations in both central and peripheral SERT binding densities (B max ) as an indication of serotonin (5-hydroxytryptamine, 5-HT) activity in humans. These studies have been made according to season (Egrise et al, 1983;Arora et al, 1984;Whitaker et al, 1984;DeMet et al, 1989;Pine et al, 1995;Spigset et al, 1998), age (Codd et al, 1988;Marazziti et al, 1989), exposure to various pharmacologic challenges (Walker and Humphries, 1984;Chen et al, 1991;Callaway et al, 1994;Mash et al, 2000;Patkar et al, 2003), time of day (Wirz-Justice et al, 1977) and also genetics (Codd et al, 1988;Sher et al, 1999;Sher, 2001).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variations in [3H]citalopram platelet binding between healthy controls and violent offenders in Finland

Callaway

Storvik

Halonen

et al. 2005

Hum. Psychopharmacol. Clin. Exp.

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Monthly binding densities (B(max)) of [3H]citalopram to the platelet serotonin transporter (SERT) was measured longitudinally over 1 year in a control group of 18 healthy Finnish male volunteers. Single platelet samples were also analysed from 33 men who were incarcerated for violent crimes during the same calendar year. A statistically significant seasonal variation in SERT B(max) was observed in both data sets, and bi-monthly floating averages for SERT B(max) were calculated and then fit to an annual sinusoidal curve for both groups. The B(max) for platelet [3H]citalopram binding showed a statistically significant (p = 0.001) seasonal variance between a winter (January-February) maximum of 1590 fmol/mg protein and a summer (July-August) minimum of 1216 fmol/mg protein for the control group, with an R2 of 70% for the annual sinusoidal curve fit. A statistically significant (p = 0.007) seasonal variance was also observed between a winter (January-February) maximum of 1980 fmol/mg protein and an autumnal (August-September) minimum of 1234 fmol/mg protein for the violent offenders, again with an R2 of 70% for the annual sinusoidal curve fit. This observation lends additional support to the idea that violent human behavior and impulsivity may be directly linked to values of SERT B(max), which can be affected by various psychoactive drugs and also varies with the natural change of seasons.

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Factors affecting serotonin uptake into human platelets

Cited by 7 publications

References 15 publications

Serotonin Transporter and Seasonal Variation in Blood Serotonin in Families with Obsessive-Compulsive Disorder

Serotonin Transporter and Seasonal Variation in Blood Serotonin in Families with Obsessive-Compulsive Disorder

The inhibitory effect of immunoglobulin E on monoamine uptake in human platelets

Seasonal variations in [3H]citalopram platelet binding between healthy controls and violent offenders in Finland

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