Factors affecting somatic embryogenesis and transformation in modern plant breeding

Deo, Pradeep C.; Tyagi, Anand P.; Taylor, Mary Anne; Harding, Robert M.; Becker, Douglas K.

doi:10.1071/sp10002

Cited by 63 publications

(48 citation statements)

References 116 publications

(146 reference statements)

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“…Em estudos de organogênese, a taxa de formação e o desenvolvimento de embriões somáticos ou zigóticos são fortemente influenciados pela composição química do meio. O balanço entre açúcares, aminoácidos e reguladores de crescimento, por exemplo, tem mostrado efeitos ora indutores, ora inibidores da formação de novos embriões (Deo et al, 2010;Kanwar et al, 2010;Karami & Saidi, 2010;Swamy et al, 2010). Assim, em sementes de Eugenia, o início da germinação de um embrião poderia modificar o balanço daqueles compostos, o que torna o meio desfavorável à diferenciação de novas plântulas.…”

Section: Introductionunclassified

Potencial de inibição da regeneração de raízes e plântulas em sementes germinantes de Eugenia pyriformis

Amador

Barbedo

2011

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o potencial de inibição da formação de raízes e plântulas, em sementes germinantes de uvaieira (Eugenia pyriformis), fragmentadas e fissuradas. As sementes foram separadas por tamanho em dois grupos. Cada grupo foi separado em dois subgrupos, um dos quais foi submetido a teste de germinação. As sementes de cada subgrupo foram submetidas a dois tipos de incisão (total ou parcial) e, em seguida, foram avaliadas quanto à produção de raízes e plântulas. Em sementes com incisão parcial, que apresentavam apenas uma plântula desenvolvida, completou-se a incisão até que as metades fossem separadas. A metade com a raiz foi descartada, e sua complementar foi colocada para germinar, para avaliação da produção de raízes e plântulas nessas frações sem raízes. Em todos os experimentos, utilizou-se o delineamento inteiramente casualizado, em arranjo fatorial 2x2 (tamanho das sementes x germinação visível) e 2x4 (tamanho das sementes x tipo de incisão). As sementes fracionadas de uvaieira apresentam potencial para regeneração de raízes e plântulas, e podem produzir mais de uma muda por semente. A germinação inicia processos de inibição da regeneração de novas raízes e plântulas na semente, e a incisão dos cotilédones pode bloquear essa inibição.Termos para indexação: fracionamento, inibidores, produção de mudas, regenerabilidade, uvaieira. Potential for regeneration inhibition of roots and seedlings in germinating Eugenia pyriformis seedsAbstract -The objective of this work was to evaluate the potential for inhibiting the formation of new roots and seedlings from germinating seeds of "uvaieira" (Eugenia pyriformis), fragmented and fissured. Seeds were separated by size into two groups. Each group was separated into two subgroups, one of which was subjected to germination test. Seeds of each subgroup were subjected to two types of incision (total or partial) and, then, evaluated as for the production of roots and seedlings. Seeds with partial incision, which had only one developed plantlet, had the incision completed until the halves were separated. The half fragment containing the root was discarded, and its complementary part was placed to germinate, in order to evaluate the production of roots and seedlings in these fractions without the roots. The design in all the experiments was completely randomized, in a 2x2 factorial arrangement (length x seed visible germination) or 2x4 (seed size x incision type). Fragmented seeds of "uvaieira" shows potential for root and seedling regeneration and can produce more than one seedling per seed. Germination starts inhibition processes of formation of new roots and plantlets, and the incision of cotyledons can block this inhibition.

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Section: Introductionunclassified

Potencial de inibição da regeneração de raízes e plântulas em sementes germinantes de Eugenia pyriformis

Amador

Barbedo

2011

Pesq. agropec. bras.

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“…Generally, exogenously applied plant growth regulators are considered as major factor for callus induction. But, endogenous level of different phytohormones in explants tissues might be a factor that influenced the required concentration of exogenously applied plant growth regulators (Deo et al 2010). Generally, auxins specially 2,4-D is reported for induction of embryogenic reponses but role of cytokinins was also reported.…”

Section: Discussionmentioning

confidence: 99%

Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers

Singh

Kashyap

Kumari

et al. 2016

Physiol Mol Biol Plants

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Somatic embryogenic system was developed in Sapindus mukorossi Gaertn. using rachis as explants from a mature tree. Explants showed callus initiation on Murashige and Skoog medium supplemented with TDZ (1-Phenyl-3-(1, 2, 3-thiadiazol-5-yl) urea), zeatin or 6-benzylaminopurine. Induction of somatic embryogenesis was achieved on both MS basal medium and MS medium supplemented with 8.88 lM 6-benzylaminopurine. Hundred percent embryogenesis was observed on MS medium supplemented with 8.88 lM 6-benzylaminopurine with maximum intensity of embryogenesis (51.92 ± 0.40 a). Maximum maturation of somatic embryos (92.86 ± 0.34 a) was observed on induction medium supplemented with 0.0378 lM abscisic and treated for 21 days. Germination of somatic embryos was maximum (77.33 ± 0.58 a) on MS medium supplemented with 8.88 lM 6-benzylaminopurine. In vitro raised plantlets were hardened, acclimatized and transferred to the field. Survival frequency of plantlets was 80 % in field conditions. The genetic fidelity of in vitro regenerated plants was also evaluated and compared with mother plant using random amplified polymorphic DNA and inter simple sequence repeat. Both markers showed similarity in molecular profile of mother plant and in vitro regenerated plants.

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“…Pertumbuhan biomassa sagu pada kultur SPS paling tinggi sehingga dapat menghasilkan jumlah embrio somatik tertinggi. Peningkatan biomassa kultur berpotensi besar untuk meningkatkan jumlah embrio somatik yang dihasilkan (Deo et al, 2010;Zhao et al, 2015). Gambar 2.…”

Section: Bobot Segar Kulturunclassified

“…Hal ini disebabkan bahan tanam yang dikulturkan berupa kalus embriogenik atau massa proembriogenik (pro embryogenic mass) yang apabila dikulturkan pada media dan kondisi yang sesuai akan segera tumbuh dan berkembang berdiferensiasi membentuk embrio somatik. Waktu induksi embrio somatik tanaman berpengaruh pada jumlah perolehan embrio somatik pada periode kultur in vitro tanaman (Deo et al, 2010;Mohajer et al, 2012). Hal ini karena semakin cepat terbentuk embrio somatik, maka semakin cepat embrio somatik mengalamai proliferasi.…”

Section: Kecepatan Induksi Atau Waktu Kalus Berdiferensiasi Membentukunclassified

Pengaruh TDZ terhadap induksi embrio somatik sagu (Metroxylon sagu Rottb.) pada tiga metode kultur berbeda (Effect of TDZ on the somatic embryo induction of sago palm (Metroxylon sagu Rottb.) in three different culture methods)

Riyadi¹,

Efendi²,

Purwoko³

et al. 2018

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Factors affecting somatic embryogenesis and transformation in modern plant breeding

Cited by 63 publications

References 116 publications

Potencial de inibição da regeneração de raízes e plântulas em sementes germinantes de Eugenia pyriformis

Potencial de inibição da regeneração de raízes e plântulas em sementes germinantes de Eugenia pyriformis

Regeneration of soapnut tree through somatic embryogenesis and assessment of genetic fidelity through ISSR and RAPD markers

Pengaruh TDZ terhadap induksi embrio somatik sagu (Metroxylon sagu Rottb.) pada tiga metode kultur berbeda (Effect of TDZ on the somatic embryo induction of sago palm (Metroxylon sagu Rottb.) in three different culture methods)

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