Factors Affecting the in vitro Multiplication of the Endemic Zingiber Curcuma haritha Mangaly and Sabu

Bejoy, M.; Dan, Mathew; Anish, N.P.

doi:10.3923/ajps.2006.847.853

Cited by 21 publications

(4 citation statements)

References 18 publications

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“…The explants cultured on medium with BAP-auxin combinations initiated callus at varying degree and it was prominent in NAA supplemented medium. Comparatively, a favorable effect of IAA over other auxins in the present study is in consonance with the report in A. calcarata [13] and Curcuma haritha [29]. The inefficiency of NAA and IBA along with BAP in the present system was similar to the recent observations in Kaempferia galanga [30].…”

Section: Resultssupporting

confidence: 95%

“…Spontaneous root induction was also noticed in more than 50% shoots grown in liquid medium (Figure 1(g)). The shoots without roots were separated from the clumps and induced roots using optimized rooting medium as in other zingibers [18,29]. The enhanced growth of shoots in liquid medium might be due to the easy and rapid intake of nutrients [31] and partial immersion of the propagules.…”

Section: Resultsmentioning

confidence: 99%

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Improved Clonal Propagation of Alpinia calcarata Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Sudha¹,

George²,

Rameshkumar³

et al. 2012

AJPS

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An efficient and improved clonal propagation of Alpinia calcarata, a commercially important medicinal plant was established on Murashige and Skoog medium. The axillary shoot proliferation was achieved with maximum 5.2 ± 0.7 shoots in 92.8% of rhizome explants in medium with 2.0 mg/L 6-benzylamiopurine (BAP) and 0.2 mg/L indole-3-acetic acid (IAA). Axillary shoot buds (60%) upon subculture for 8 weeks in the same medium produced multiple shoot initials (12.1 ± 0.4) mediated with meristemoids (4.0 ± 0.5) and callus. A gradual reduction in the concentration of BAP or elimination of IAA was required for rapid induction of normal plants devoid of callus from propagules during subsequent subculture. Single clump of 3-4 multiple shoot initials during second subculture on medium with 1.0 mg/L BAP and 0.1 mg/L IAA yielded an average of 21 shoots which was best among different propagules tried. The shoot multiplication rate was further enhanced to 32 shoots when the similar propagules passed to third subculture on medium with 1.0 mg/L BAP alone. Clumps of multiple shoot initials upon subculture on medium with 1.0 mg/L BAP alone exhibited 10 fold multiplication rates. Use of liquid medium in culture bottles with polypropylene caps supported fast growth of the shoots and spontaneous root formation on 50% of the shoots. Shoots transferred to half-strength MS liquid medium with 0.2 mg/L of IAA and IBA was optimum for maximum roots (8.14 ± 1.34) in 100% shoots. The rooted plants were hardened in mist chamber showed 95% survival and well established in the field. The acclimatized plants showed rhizome formation after 4-6 weeks of growth under shade house. Volatile chemicals profile of the leaves, rhizome and root of the in vitro and conventionally propagated plants analyzed by gas chromatography-mass spectrometry were qualitatively and quantitatively similar. The analysis of growth characteristics of 36 month old in vitro and conventionally propagated plants showed a 50% increment of rhizome fresh biomass with prolific root and leaf growth in the former than the latter ones. The protocol described herein will have practical applications for the large scale production of uniform efficient plants for commercial cultivation of A. calcarata

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Section: Resultssupporting

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Improved Clonal Propagation of Alpinia calcarata Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Sudha¹,

George²,

Rameshkumar³

et al. 2012

AJPS

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“…The effect of cytokinins on shoot multiplication is presented in Table 1. The notable efficiency of BAP in inducing effective multiplication aligned with findings from other micropropagation studies carried out on Curcuma species like C. haritha (Bejoy et al 2006), C. mangga (Raihana et al 2011), C. caesia (Bharalee et al 2005), C. zedoaria (Loc et al 2005), C. vamana (Bejoy et al 2012), etc.…”

Section: Initiation Of Culture and Multiplicationsupporting

confidence: 82%

6 – BENZYLAMINOPURINE INDUCES HIGH-FREQUENCY MULTIPLICATION IN VULNERABLE Curcuma pseudomontana J. Graham.: A POTENTIAL EX VIVO CONSERVATION TOOL

Vaze,

Pramod Hurkadale,

Hegde

2024

BIOTROPIA

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A rapid high-frequency multiplication protocol is designed for Curcuma pseudomontana J. Graham, belonging to the family Zingiberaceae, an endemic species to the Western Ghats of India. The IUCN Red List of Threatened Taxa mentions this species as vulnerable due to multiple underlying causes. The Plant is extensively used in traditional and tribal medicine. The species has suffered habitat loss due to uncontrolled use for tribal medicine leading to a 30% loss in the last decade. This study is planned with a specific objective to conserve the species, and this is the first-ever report of micropropagation of Curcuma pseudomontana J. Graham using in vitro multiplication. An efficient rapid protocol for Micropropagation is developed using rhizome bud explants. The explants are transferred from MS basal medium onto the MS medium fortified with BAP, KN, TDZ at a varying concentration range. The maximum shoot induction is observed in MS medium enriched with BAP 2mg L-1resulting in 9.66 ±2.08 number of shoots per explant with a shoot length of 6.40 ±0.36cm. The root induction response is studied by aseptically transferring the shoots onto MS medium fortified with NAA, IBA, and IAA at varying concentration. Maximum root length and root number is recorded in MS supplemented with 1- Naphthalene Acetic Acid (NAA) at 0.5 mg L-1. However, a 100 % root induction frequency is observed in all the samples under study. The rooted plantlets are removed from the culture flasks and transferred into hardening media containing 1:1:1 ratio of Sand: Soil: Cocopeat. The hardened plants are healthy and disease-free and showed a 92% survival after acclimatization.

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“…Increasing concentration of agar to 10 g/l reduces Curcuma haritha L. shoot number and length. This may be due to the reduced uptake of mineral nutrients and organic matters, due to higher rigidity of the medium [38]. Therefore, the level of agar in the medium should be such that it minimizes the water loss and allows the good diffusion of nutrient elements.…”

Section: Effects Of Agar Concentrationmentioning

confidence: 99%

Medium effects on micropropagation and genetic stability of Citrullus lanatus oleaginous type

Gnamien¹,

Bi²,

Kouadio³

et al. 2013

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To regenerate adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were tested. Shoot induction proved to depend on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar allowed the production of numerous shoots without a callus phase. After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots with an average of 12.26 shoots per explant and a mean shoot length of 17.13 mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal parts ranged from 54% to 96%. Rooted plantlets were acclimatized and transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of the in-vitro-regenerated plantlets by means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets and mother plants.

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Factors Affecting the in vitro Multiplication of the Endemic Zingiber Curcuma haritha Mangaly and Sabu

Cited by 21 publications

References 18 publications

Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

6 – BENZYLAMINOPURINE INDUCES HIGH-FREQUENCY MULTIPLICATION IN VULNERABLE Curcuma pseudomontana J. Graham.: A POTENTIAL EX VIVO CONSERVATION TOOL

Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type

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Factors Affecting the in vitro Multiplication of the Endemic Zingiber Curcuma haritha Mangaly and Sabu

Cited by 21 publications

References 18 publications

Improved Clonal Propagation of &lt;i&gt;Alpinia calcarata&lt;/i&gt; Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Improved Clonal Propagation of &lt;i&gt;Alpinia calcarata&lt;/i&gt; Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

6 – BENZYLAMINOPURINE INDUCES HIGH-FREQUENCY MULTIPLICATION IN VULNERABLE Curcuma pseudomontana J. Graham.: A POTENTIAL EX VIVO CONSERVATION TOOL

Medium effects on micropropagation and genetic stability of &lt;i&gt;Citrullus lanatus&lt;/i&gt; oleaginous type

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Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Improved Clonal Propagation of <i>Alpinia calcarata</i> Rosc., a Commercially Important Medicinal Plant and Evaluation of Chemical Fidelity through Comparison of Volatile Compounds

Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type